About: Optimization of fixation methods for observation of bacterial cell morphology and surface ultrastructures by atomic force microscopy

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About: Optimization of fixation methods for observation of bacterial cell morphology and surface ultrastructures by atomic force microscopy Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Optimization of fixation methods for observation of bacterial cell morphology and surface ultrastructures by atomic force microscopy
Creator	Zhang, Tong Chao, Yuanqing
Source	PMC
abstract	Fixation ability of five common fixation solutions, including 2.5% glutaraldehyde, 10% formalin, 4% paraformaldehyde, methanol/acetone (1:1), and ethanol/acetic acid (3:1) were evaluated by using atomic force microscopy in the present study. Three model bacteria, i.e., Escherichia coli, Pseudomonas putida, and Bacillus subtilis were applied to observe the above fixation methods for the morphology preservation of bacterial cells and surface ultrastructures. All the fixation methods could effectively preserve cell morphology. However, for preserving bacterial surface ultrastructures, the methods applying aldehyde fixations performed much better than those using alcohols, since the alcohols could detach the surface filaments (i.e., flagella and pili) significantly. Based on the quantitative and qualitative assessments, the 2.5% glutaraldehyde was proposed as a promising fixation solution both for observing morphology of both bacterial cell and surface ultrastructures, while the methonal/acetone mixture was the worst fixation solution which may obtain unreliable results. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00253-011-3551-5) contains supplementary material, which is available to authorized users.
has issue date	2011-09-01(xsd:dateTime)
bibo:doi	10.1007/s00253-011-3551-5
bibo:pmid	21881891
has license	no-cc
sha1sum (hex)	af23b8413f412e60198f3ed17aed80d528508fef
schema:url	https://doi.org/10.1007/s00253-011-3551-5
resource representing a document's title	Optimization of fixation methods for observation of bacterial cell morphology and surface ultrastructures by atomic force microscopy
has PubMed Central identifier	PMC3181414
has PubMed identifier	21881891
schema:publication	Appl Microbiol Biotechnol
resource representing a document's body	covid:af23b8413f412e60198f3ed17aed80d528508fef#body_text
is schema:about of	named entity 'fixation' named entity 'glutaraldehyde' named entity 'ethanol' named entity 'flagella' named entity 'atomic force microscopy' named entity 'cell' named entity 'ASSESSMENTS' named entity '10%' named entity 'METHANOL' named entity 'mixture' named entity 'morphology' named entity 'Pseudomonas' named entity 'quantitative' named entity 'All' named entity 'assessments' named entity 'surface' named entity 'bacterial' named entity 'observing' named entity 'aldehyde' named entity 'atomic force microscopy' named entity 'bacteria' named entity 'pili' named entity 'methanol' named entity 'formalin' named entity 'bacteria' named entity 'dehydration' named entity 'tapping mode' named entity 'solubility' named entity 'AFM' named entity 'adhesion' named entity 'Pelling' named entity 'flagella' named entity 'formalin solution' named entity 'acetone' named entity 'Petri dishes' named entity 'cantilever' named entity 'morphology' named entity 'sonication' named entity 'methanol' named entity 'morphology' named entity 'spring constant' named entity 'membrane lipids' named entity 'depolymerized' named entity 'flagella' named entity 'oscillation' named entity 'bacteria' named entity 'cantilever' named entity 'acetone' named entity 'flagella' named entity 'AFM' named entity 'tapping mode' named entity '5.5' named entity 'acetic acid' named entity 'cell morphology' named entity 'morphology' named entity '12 nm' named entity 'ethanol' named entity 'macromolecules' named entity 'methanol' named entity 'flagella' named entity 'contact mode' named entity 'cell surface' named entity 'flagella' named entity 'B. subtilis' named entity 'flagella' named entity 'ATCC' named entity 'AFM' named entity 'bacterial flagella' named entity 'EPS' named entity 'EPS' named entity 'ethanol' named entity 'salt crystals' named entity 'paraformaldehyde' named entity 'methanol' named entity 'formalin' named entity 'PBS' named entity 'flagella' named entity 'E. coli' named entity 'cantilever' named entity 'membrane lipids'

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