No significant differences in mean VH : CD ratio and clinical symptoms ( diarrhea , vomiting , dehydration , and anorexia ) were observed between the PEDV / PGAR - infected and PEDV - infected groups of piglets at 1 , 2 and 3 DPI ; however , at 2 and 3 DPI , PGAR was detected in all fecal samples by RT - PCR and virus isolation .