About: Construction, expression and antiviral activity analysis of recombinant adenovirus expressing human IFITM3 in vitro

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Construction, expression and antiviral activity analysis of recombinant adenovirus expressing human IFITM3 in vitro
Creator	Chen, Jing Jin, Ningyi Tian, Mingyao Ren, Linzhu Bai, Jieying Cao, Tingting Du, Shouwen Fu, Tingting Hao, Penfeng Jiang, Yinyue Jiang, Yuhang Li, Chang Li, Tiyuan Song, Lina Wang, Maopeng Wang, Yuhang Wu, Shipin Xu, Wang
source	Elsevier; Medline; PMC
abstract	Abstract Interferon-inducible transmembrane protein 3 (IFITM3) inhibits the replication of multiple pathogenic viruses by blocking their entry. In this study, we constructed a shuttle plasmid, harboring human IFITM3. Thereafter, recombinant adenovirus rAd5-IFITM3 was obtained by co-transfection of the linearized viral backbone vector pAd5 and the shuttle plasmid. The results showed that human IFITM3 did not affect the assembly and morphogenesis of progeny adenovirus. Human IFITM3 can be expressed in both A549 and MDCK cells in a time dependent manner. Furthermore, cells infected with rAd5-IFITM3 at a multiplicity of infection (MOI) of 100 for 24 h were challenged with avian influenza virus (AIV) H5N1 at an MOI of 1 for 6, 12 and 24 h. Rates of H5N1 infection in rAd5-IFITM3 cells were significantly decreased at 24 h post-infection (hpi), in a time dependent manner, compared with that of wild type wtAd5-infected cells. The expressions of viral genes were significantly inhibited at transcriptional and translational levels at 6 and 12 hpi. These results suggest that IFITM3 can suppress H5N1 replication in the early stage of the infection, which may be used as a promise agent against H5N1 infection in vivo.
has issue date	2019-06-15(xsd:dateTime)
bibo:doi	10.1016/j.ijbiomac.2019.03.161
bibo:pmid	30914370
has license	els-covid
sha1sum (hex)	b0115f2908588665fcef5563df8a5eaaca99f5b8
schema:url	https://doi.org/10.1016/j.ijbiomac.2019.03.161
resource representing a document's title	Construction, expression and antiviral activity analysis of recombinant adenovirus expressing human IFITM3 in vitro
has PubMed Central identifier	PMC7112391
has PubMed identifier	30914370
schema:publication	International Journal of Biological Macromolecules
resource representing a document's body	covid:b0115f2908588665fcef5563df8a5eaaca99f5b8#body_text
is schema:about of	named entity 'human' named entity 'cells' named entity 'infection' named entity 'infected' named entity 'PLASMID' named entity 'IN VIVO' named entity 'USED' named entity 'CELLS' named entity 'EARLY STAGE' named entity 'MAY BE' named entity 'PROMISE' named entity 'EXPRESSED' named entity 'STUDY' named entity 'TRANSLATIONAL' named entity 'VIRAL' named entity 'TIME' named entity 'MULTIPLE' named entity 'DEPENDENT' named entity 'THESE' named entity 'VIRAL GENES' named entity 'PAD5' named entity 'TRANSMEMBRANE PROTEIN 3' named entity 'RATES' named entity 'EXPRESSION' named entity '100' named entity 'ACTIVITY ANALYSIS' named entity 'RECOMBINANT ADENOVIRUS' named entity 'INFECTION' named entity 'AFFECT' named entity 'INHIBITED' named entity 'VIRUSES' named entity 'BLOCKING' named entity 'RECOMBINANT ADENOVIRUS' named entity 'RAD5' named entity 'WILD TYPE' named entity 'VECTOR' named entity 'HPI' named entity 'BACKBONE' named entity 'AVIAN INFLUENZA VIRUS' named entity 'HUMAN' named entity 'ENTRY' named entity 'REPLICATION' named entity 'IFITM3' named entity 'HARBORING' named entity 'MDCK CELLS' named entity 'POST' named entity 'INDUCIBLE' named entity 'CONSTRUCTION' named entity 'ASSEMBLY' named entity 'H5N1' named entity 'MULTIPLICITY' named entity 'SUPPRESS' named entity 'DECREASED' named entity 'DID' named entity 'TRANSCRIPTIONAL' named entity 'LEVELS' named entity 'SHUTTLE' named entity 'OBTAINED BY' named entity 'MORPHOGENESIS' named entity 'TRANSFECTION' named entity 'CONSTRUCTED' named entity 'PROGENY' named entity 'A549' named entity 'INTERFERON'

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