About: Seroepidemiology of Breda virus in cattle using ELISA

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Seroepidemiology of Breda virus in cattle using ELISA
Creator	Horzinek, M Koopmans, M Van Den Boom, U Woode, G
Source	PMC
abstract	Two direct blocking enzyme linked immunosorbent assays (ELISA) for the detection of antibodies to Breda virus in sera of cattle were compared. An ELISA with consecutive addition of antigen and test serum to an antibody-coated plate gave higher positive: negative absorbence ratios than an ELISA in which antigen and test serum were added simultaneously. Sera collected from breeding and fattening herds in The Netherlands (n = 1313) and the F.R.G. (n = 716) were tested, and antibodies to Breda virus were demonstrated in 94% of adult cattle. Ninety percent of newborn calves had high levels of maternal antibodies, which waned until the age of 3 months. Active seroconversion occurred between 7 and 24 months in most animals.
has issue date	2002-11-13(xsd:dateTime)
bibo:doi	10.1016/0378-1135(89)90069-2
bibo:pmid	2718353
has license	no-cc
sha1sum (hex)	b086d1e195b99405f30cd5fa1cede945a18befcf
schema:url	https://doi.org/10.1016/0378-1135%2889%2990069-2
resource representing a document's title	Seroepidemiology of Breda virus in cattle using ELISA
has PubMed Central identifier	PMC7117312
has PubMed identifier	2718353
schema:publication	Vet Microbiol
resource representing a document's body	covid:b086d1e195b99405f30cd5fa1cede945a18befcf#body_text
is schema:about of	named entity 'SEROEPIDEMIOLOGY' named entity 'higher' named entity 'HIGHER' named entity 'RATIOS' named entity 'ADDED' named entity 'ELISA' named entity 'BLOCKING' named entity 'DEN' named entity 'GAVE' named entity 'USING' named entity 'SEROEPIDEMIOLOGY' named entity 'BREDA VIRUS' named entity 'COMPARED' named entity 'USING' named entity 'ANTIBODY-COATED PLATE' named entity 'VAN' named entity 'ENZYME' named entity 'NEGATIVE' named entity 'ANTIGEN' named entity 'POSITIVE' named entity 'SERUM' named entity 'CONSECUTIVE' named entity 'DETECTION' named entity 'ADDITION' named entity 'CATTLE' named entity 'ELISA' named entity 'BREDA VIRUS' named entity 'ANTIBODIES' named entity 'TEST' named entity 'SERA' named entity 'SIMULTANEOUSLY' named entity 'VET' named entity 'BOOM' named entity 'CATTLE' named entity 'DIRECT' named entity 'IMMUNOSORBENT' covid:arg/b086d1e195b99405f30cd5fa1cede945a18befcf named entity 'antigen' named entity 'Breda' named entity 'antigen' named entity 'virus' named entity 'serum' named entity 'Cattle' named entity 'addition' named entity 'virus' named entity 'Serological' named entity 'bovine' named entity 'milk production' named entity 'epidemiological' named entity 'serum' named entity 'antibodies' named entity 'colostrum' named entity 'antigen' named entity 'positive and negative' named entity 'EDTA' named entity 'cattle' named entity 'correlation coefficient' named entity 'colostrum' named entity 'Hessen' named entity 'antibody' named entity 'immunoglobulin' named entity 'pathogenicity' named entity 'Germany' named entity 'diarrhea' named entity 'cattle' named entity 'dysentery' named entity 'bovine coronavirus' named entity 'colostrum' named entity 'infection' named entity 'newborn calves' named entity 'sucrose' named entity 'serum' named entity 'hemagglutinating' named entity '1:400' named entity 'phosphate buffered saline' named entity 'colostrum' named entity 'Arnsberg' named entity 'Serotype'

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