About: Porcine Epidemic Diarrhea Virus (PEDV) ORF3 Interactome Reveals Inhibition of Virus Replication by Cellular VPS36 Protein

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Porcine Epidemic Diarrhea Virus (PEDV) ORF3 Interactome Reveals Inhibition of Virus Replication by Cellular VPS36 Protein Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Porcine Epidemic Diarrhea Virus (PEDV) ORF3 Interactome Reveals Inhibition of Virus Replication by Cellular VPS36 Protein
Creator	Jongkaewwattana, Anan Kaewborisuth, Challika Roytrakul, Sittiruk Yingchutrakul, Yodying
source	Medline; PMC
abstract	The accessory protein ORF3 of porcine epidemic diarrhea virus (PEDV) has been proposed to play a key role in virus replication. However, our understanding of its function regarding virus and host interaction is still limited. In this study, we employed immunoprecipitation and mass spectrometry to screen for cellular interacting partners of ORF3. Gene ontology analysis of the host interactome highlighted the involvement of ORF3 in endosomal and immune signaling pathways. Among the identified ORF3-interacting proteins, the vacuolar protein-sorting-associated protein 36 (VPS36) was assessed for its role in PEDV replication. VPS36 was found to interact with ORF3 regardless of its GLUE domain. As a result of VPS36–ORF3 interaction, PEDV replication was substantially suppressed in cells overexpressing VPS36. Interestingly, the ORF3 protein expression was diminished in VPS36-overexpressing cells, an effect that could not be restored by treatment of lysosomal inhibitors. In addition, disruption of endogenously-expressed VPS36 by siRNA could partially augment PEDV replication. Taken together, our study provides mechanistic insights into the contribution of ORF3 in PEDV replication.
has issue date	2019-04-24(xsd:dateTime)
bibo:doi	10.3390/v11040382
bibo:pmid	31022991
has license	cc-by
sha1sum (hex)	b2126ad45f5a952f27851ec2ef42245a3d8f09c4
schema:url	https://doi.org/10.3390/v11040382
resource representing a document's title	Porcine Epidemic Diarrhea Virus (PEDV) ORF3 Interactome Reveals Inhibition of Virus Replication by Cellular VPS36 Protein
has PubMed Central identifier	PMC6521123
has PubMed identifier	31022991
schema:publication	Viruses
resource representing a document's body	covid:b2126ad45f5a952f27851ec2ef42245a3d8f09c4#body_text
is schema:about of	named entity 'restored' named entity 'study' named entity 'interacting' covid:arg/b2126ad45f5a952f27851ec2ef42245a3d8f09c4 named entity 'interacting' named entity 'augment' named entity 'vacuolar' named entity 'virus' named entity 'Cellular' named entity 'Gene ontology' named entity 'protein' named entity 'signaling pathways' named entity 'PEDV' named entity 'siRNA' named entity 'PEDV' named entity 'Porcine Epidemic Diarrhea Virus' named entity 'Interactome' named entity 'amino acids' named entity 'VPS36' named entity 'IKBKB' named entity 'ubiquitination' named entity 'amino acids' named entity 'ubiquitin' named entity 'protein' named entity 'structural proteins' named entity 'coronaviruses' named entity 'PEDV' named entity 'lysis buffer' named entity 'lysosome' named entity 'protein interaction' named entity 'viral RNA' named entity 'physical interaction' named entity 'immunoprecipitation' named entity 'microplate reader' named entity 'SDS-PAGE' named entity 'incubation' named entity 'endoplasmic reticulum' named entity 'N-terminus' named entity 'transfected' named entity 'ATCC' named entity 'ubiquitin-proteasome pathway' named entity 'transfected' named entity 'VPS36' named entity 'host cells' named entity 'plasmid' named entity 'LC-MS/MS' named entity 'agarose' named entity 'protein' named entity 'structural proteins' named entity 'ubiquitination' named entity 'pull-down' named entity 'tween 20' named entity 'lysosome' named entity 'pathogens' named entity 'mzXML' named entity 'DAPI' named entity 'protein degradation' named entity 'Real-Time PCR' named entity 'aminopeptidase' named entity 'protein' named entity 'VPS36' named entity 'LC-MS/MS' named entity 'cell surface' named entity 'sulfhydryl' named entity 'Cell lysates' named entity 'PEDV' named entity 'myc' named entity 'VPS36' named entity 'silver staining' named entity 'PEDV' named entity 'VPS36' named entity 'VPS36' named entity 'PEDV' named entity 'lysates' named entity 'acetone' named entity 'bead' named entity 'PANTHER' named entity 'antibodies'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software