About: A new approach for diagnosis of bovine coronavirus using a reverse transcription recombinase polymerase amplification assay

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About: A new approach for diagnosis of bovine coronavirus using a reverse transcription recombinase polymerase amplification assay Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A new approach for diagnosis of bovine coronavirus using a reverse transcription recombinase polymerase amplification assay
Creator	Amer, H Wahed, El Abd, A Almajhdi, F Hufert, F Shalaby, M Weidmann, M
Source	Elsevier; Medline; PMC
abstract	Abstract Bovine coronavirus (BCoV) is an economically significant cause of calf scours and winter dysentery of adult cattle, and may induce respiratory tract infections in cattle of all ages. Early diagnosis of BCoV helps to diminish its burden on the dairy and beef industry. Real-time RT-PCR assay for the detection of BCoV has been described, but it is relatively expensive, requires well-equipped laboratories and is not suitable for on-site screening. A novel assay, using reverse transcription recombinase polymerase amplification (RT-RPA), for the detection of BCoV is developed. The BCoV RT-RPA was rapid (10–20min) and has an analytical sensitivity of 19 molecules. No cross-reactivity with other viruses causing bovine gastrointestinal and/or respiratory infections was observed. The assay performance on clinical samples was validated by testing 16 fecal and 14 nasal swab specimens and compared to real-time RT-PCR. Both assays provided comparable results. The RT-RPA assay was significantly more rapid than the real-time RT-PCR assay. The BCoV RT-RPA constitutes a suitable accurate, sensitive and rapid alternative to the common measures used for BCoV diagnosis. In addition, the use of a portable fluorescence reading device extends its application potential to use in the field and point-of-care diagnosis.
has issue date	2013-11-30(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2013.06.027
bibo:pmid	23811231
has license	els-covid
sha1sum (hex)	b2342adca6443124098da5af97e1a9639da8be46
schema:url	https://doi.org/10.1016/j.jviromet.2013.06.027
resource representing a document's title	A new approach for diagnosis of bovine coronavirus using a reverse transcription recombinase polymerase amplification assay
has PubMed Central identifier	PMC7113639
has PubMed identifier	23811231
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:b2342adca6443124098da5af97e1a9639da8be46#body_text
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