About: Functional analysis of the receptor binding domain of SARS coronavirus S1 region and its monoclonal antibody

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About: Functional analysis of the receptor binding domain of SARS coronavirus S1 region and its monoclonal antibody Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Functional analysis of the receptor binding domain of SARS coronavirus S1 region and its monoclonal antibody
Creator	Suzuki, Yasuhiko Kim, Hyun Keigo Shibayama, Hong Kim, Uck Nobutaka, • Yeongjin, • Youn, Wakamiya
Source	PMC
abstract	Severe acute respiratory syndrome (SARS) is caused by the SARS coronavirus (CoV). The spike protein of SARS-CoV consists of S1 and S2 domains, which are responsible for virus binding and fusion, respectively. The receptor-binding domain (RBD) positioned in S1 can specifically bind to angiotensin-converting enzyme 2 (ACE2) on target cells, and ACE2 regulates the balance between vasoconstrictors and vasodilators within the heart and kidneys. Here, a recombinant fusion protein containing 193-amino acid RBD (residues 318–510) and glutathione S-transferase were prepared for binding to target cells. Additionally, monoclonal RBD antibodies were prepared to confirm RBD binding to target cells through ACE2. We first confirmed that ACE2 was expressed in various mouse cells such as heart, lungs, spleen, liver, intestine, and kidneys using a commercial ACE2 polyclonal antibody. We also confirmed that the mouse fibroblast (NIH3T3) and human embryonic kidney cell lines (HEK293) expressed ACE2. We finally demonstrated that recombinant RBD bound to ACE2 on these cells using a cellular enzyme-linked immunosorbent assay and immunoassay. These results can be applied for future research to treat ACE2-related diseases and SARS.
has issue date	2014-04-16(xsd:dateTime)
bibo:doi	10.1007/s13258-014-0186-9
has license	no-cc
sha1sum (hex)	b23503d8506882ea3e3e90c80f86e1ebaac1ffa6
schema:url	https://doi.org/10.1007/s13258-014-0186-9
resource representing a document's title	Functional analysis of the receptor binding domain of SARS coronavirus S1 region and its monoclonal antibody
has PubMed Central identifier	PMC7097624
schema:publication	Genes Genomics
resource representing a document's body	covid:b23503d8506882ea3e3e90c80f86e1ebaac1ffa6#body_text
is schema:about of	named entity 'DOMAIN' named entity 'RECEPTOR BINDING' named entity 'CELLULAR' named entity 'BOUND' named entity 'APPLIED' named entity 'PREPARED' named entity 'USING' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'THESE' named entity 'PROTEIN ' named entity 'INTESTINE' named entity 'RECOMBINANT' named entity 'CELLS' named entity 'CONFIRMED' named entity 'VASODILATORS' named entity 'ACE2' named entity 'LUNGS' named entity 'CAUSED BY' named entity 'RESPONSIBLE' named entity 'IMMUNOASSAY' named entity 'FUSION' named entity 'HEART' named entity 'TREAT' named entity 'REGION' named entity 'VIRUS BINDING' named entity 'MOUSE' named entity 'SPIKE PROTEIN' named entity 'EXPRESSED' named entity 'MONOCLONAL ANTIBODY' named entity 'SARS CORONAVIRUS' named entity 'ANGIOTENSIN-CONVERTING ENZYME 2' named entity 'POLYCLONAL ANTIBODY' named entity 'CELL LINES' named entity 'MOUSE FIBROBLAST' named entity 'RESEARCH' named entity 'LIVER' named entity 'VASOCONSTRICTORS' named entity 'MONOCLONAL' named entity 'BINDING' named entity 'FUSION PROTEIN' named entity 'DOMAIN' named entity 'ANTIBODIES' named entity 'RESULTS' named entity 'VARIOUS' named entity 'ASSAY' named entity 'GLUTATHIONE S-TRANSFERASE' named entity 'CONTAINING' named entity 'DISEASES' named entity 'FUTURE' named entity 'SPLEEN' named entity 'KIDNEYS' named entity '510' named entity 'HUMAN EMBRYONIC KIDNEY CELL' named entity 'HERE' named entity 'TARGET CELLS' named entity 'FUNCTIONAL ANALYSIS' named entity 'ITS' named entity 'COMMERCIAL' named entity 'RBD' named entity 'BIND' named entity 'DEMONSTRATED' named entity 'BALANCE' named entity 'SARS' named entity 'CONFIRM' named entity 'SARS CORONAVIRUS' named entity 'AMINO ACID' named entity 'IMMUNOSORBENT' named entity 'RECEPTOR' named entity 'RBD' named entity 'amino acid' named entity 'immunosorbent assay' named entity 'vasodilators' named entity 'immunoassay' named entity 'glutathione S-transferase' named entity 'RBD' named entity 'binding domain' named entity 'Functional analysis'

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