About: Detection of SARS-CoV-2 RNA by multiplex RT-qPCR

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About: Detection of SARS-CoV-2 RNA by multiplex RT-qPCR Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Detection of SARS-CoV-2 RNA by multiplex RT-qPCR
Creator	Iwasaki, Akiko Brackney, Doug Grubaugh, Nathan Omer, Saad Ko, Albert Ott, Isabel Petrone, Mary Vogels, Chantal Wyllie, Anne Team, Research Casanovas-Massana, Arnau Cruz, Charles Earnest, Rebecca Farhadian, Shelli Israelow, Benjamin Lapidus, Sarah Lu, Peiwen Moore, Adam Tokuyama, Maria Venkataraman, Arvind Muenker, M Impact, Yale Kudo, Eriko
source	BioRxiv
abstract	The current RT-qPCR assay recommended for SARS-CoV-2 testing in the United States requires analysis of three genomic targets per sample: two viral and one host. To simplify testing and reduce the volume of required reagents, we developed a multiplex RT-qPCR assay to detect SARS-CoV-2 in a single reaction. We used existing N1, N2, and RP primer and probe sets by the CDC, but substituted fluorophores to allow multiplexing of the assay. The cycle threshold (Ct) values of our multiplex RT-qPCR were comparable to those obtained by the singleplex assay adapted for research purposes. Low copies (>500 copies / reaction) of SARS-CoV-2 RNA were consistently detected by the multiplex RT-qPCR. Our novel multiplex RT-qPCR improves upon current singleplex diagnostics by saving reagents, costs, time and labor.
has issue date	2020-06-17(xsd:dateTime)
bibo:doi	10.1101/2020.06.16.155887
has license	biorxiv
sha1sum (hex)	b25cfe8502860ae98d7f3b6d2c8c6ed12fffa820
schema:url	https://doi.org/10.1101/2020.06.16.155887
resource representing a document's title	Detection of SARS-CoV-2 RNA by multiplex RT-qPCR
schema:publication	bioRxiv
resource representing a document's body	covid:b25cfe8502860ae98d7f3b6d2c8c6ed12fffa820#body_text
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