About: Exploration of the metal coordination region of concanavalin A for its interaction with human norovirus

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About: Exploration of the metal coordination region of concanavalin A for its interaction with human norovirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Exploration of the metal coordination region of concanavalin A for its interaction with human norovirus
Creator	Choi, Jong-Soon Kwon, Joseph Kim, Duwoon Lee, Hee-Min Oh, Kyung-Seo Vaidya, Bipin Kim, Dongkyun Kim, Min Ki, Ah Kim, Sung Lee, Jae Protzman, Rachael
Source	Elsevier; Medline; PMC
abstract	Abstract Rapid methods for the detection and clinical treatment of human norovirus (HuNoV) are needed to control foodborne disease outbreaks, but reliable techniques that are fast and sensitive enough to detect small amounts of HuNoV in food and aquatic environments are not yet available. We explore the interactions between HuNoV and concanavalin A (Con A), which could facilitate the development of a sensitive detection tool for HuNoV. Biophysical studies including hydrogen/deuterium exchange (HDX) mass spectrometry and surface plasmon resonance (SPR) revealed that when the metal coordinated region of Con A, which spans Asp16 to His24, is converted to nine alanine residues (mCon AMCR), the affinity for HuNoV (GII.4) diminishes, demonstrating that this Ca2+ and Mn2+ coordinated region is responsible for the observed virus-protein interaction. The mutated carbohydrate binding region of Con A (mCon ACBR) does not affect binding affinity significantly, indicating that MCR of Con A is a major region of interaction to HuNoV (GII.4). The results further contribute to the development of a HuNoV concentration tool, Con A-immobilized polyacrylate beads (Con A-PAB), for rapid detection of genotypes from genogroups I and II (GI and GII). This method offers many advantages over currently available methods, including a short concentration time. HuNov (GI and GII) can be detected in just 15 min with 90% recovery through Con A-PAB application. In addition, this method can be used over a wide range of pH values (pH 3.0 – 10.0). Overall, this rapid and sensitive detection of HuNoV (GI and GII) will aid in the prevention of virus transmission pathways, and the method developed here may have applicability for other foodborne viral infections.
has issue date	2017-06-30(xsd:dateTime)
bibo:doi	10.1016/j.biomaterials.2017.03.006
bibo:pmid	28288347
has license	els-covid
sha1sum (hex)	b27bd7226c65b62ca0a72fa49ab96c7d877234c5
schema:url	https://doi.org/10.1016/j.biomaterials.2017.03.006
resource representing a document's title	Exploration of the metal coordination region of concanavalin A for its interaction with human norovirus
has PubMed Central identifier	PMC7112440
has PubMed identifier	28288347
schema:publication	Biomaterials
resource representing a document's body	covid:b27bd7226c65b62ca0a72fa49ab96c7d877234c5#body_text
is schema:about of	named entity 'wide range' named entity 'region' named entity 'clinical treatment' named entity 'interaction' named entity 'GII' named entity 'genotypes' named entity 'sensitive' named entity 'surface plasmon' named entity 'detection' named entity 'CONVERTED' named entity 'EXPLORATION' named entity 'STUDIES' named entity 'WIDE' named entity 'REVEALED' named entity 'EXCHANGE' named entity 'METAL' named entity 'PATHWAYS' named entity 'CONTRIBUTE ' named entity 'MASS SPECTROMETRY' named entity 'RESPONSIBLE' named entity 'OVERALL' named entity 'FAST' named entity 'TIME' named entity 'RELIABLE' named entity 'CLINICAL TREATMENT' named entity 'COORDINATED' named entity 'DETECTION' named entity 'JUST' named entity 'TO DETECT' named entity 'MAY HAVE' named entity 'OBSERVED' named entity 'GENOTYPES' named entity 'E 10' named entity 'FOOD' named entity 'SHORT' named entity 'AFFINITY' named entity 'INDICATING' named entity 'SURFACE PLASMON RESONANCE' named entity 'REGION' named entity 'RECOVERY' named entity 'DISEASE OUTBREAKS' named entity 'DETECTED' named entity 'BEADS' named entity 'DEVELOPMENT' named entity 'NEEDED' named entity 'INTERACTION' named entity 'AID' named entity '90%' named entity 'APPLICATION' named entity 'TO CONTROL' named entity 'AMOUNTS' named entity 'VIRAL INFECTIONS' named entity 'A MAJOR' named entity 'MUTATED' named entity 'CBR' named entity 'POLYACRYLATE' named entity 'BIOPHYSICAL' named entity 'HYDROGEN' named entity 'MIN' named entity 'USED' named entity 'CONCANAVALIN A' named entity 'ITS' named entity 'COORDINATION' named entity 'HUMAN NOROVIRUS' named entity 'METAL' named entity 'IS A' named entity 'SENSITIVE' named entity 'RESULTS' named entity 'PREVENTION OF' named entity 'INTERACTION'

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