About: A reverse transcription loop-mediated isothermal amplification method for rapid detection of bovine viral diarrhea virus

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About: A reverse transcription loop-mediated isothermal amplification method for rapid detection of bovine viral diarrhea virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A reverse transcription loop-mediated isothermal amplification method for rapid detection of bovine viral diarrhea virus
Creator	Wang, Xiuqing Fan, Qing Deng, Xianwen Liu, Jiabo Pang, Yaoshan Peng, Yi Xie, Liji Xie, Zhiqin Xie, Zhixun
Source	Elsevier; Medline; PMC
abstract	A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed and optimized to detect bovine viral diarrhea viral (BVDV) RNA. The RT-LAMP assay is highly sensitive and able to detect 4.67 × 10(0) copies of BVDV RNA. Additionally, the RT-LAMP method is capable of detecting both genotypes of BVDV. No cross-reaction with other bovine viruses was observed. The ability of RT-LAMP to detect BVDV RNA from bovine fecal swabs was also evaluated. Of the 88 fecal swabs, 38 were found to be positive by RT-LAMP assay, whereas 39 were positive by real-time RT-PCR. Taken together, the BVDV specific RT-LAMP method is highly specific and sensitive and can be used as a rapid and direct diagnostic assay for testing clinical samples.
has issue date	2012-08-27(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2012.08.007
bibo:pmid	22947692
has license	no-cc
sha1sum (hex)	b2cf96174de10c8c3f143ee7ca4ac8b92778c693
schema:url	https://doi.org/10.1016/j.jviromet.2012.08.007
resource representing a document's title	A reverse transcription loop-mediated isothermal amplification method for rapid detection of bovine viral diarrhea virus
has PubMed Central identifier	PMC7112856
has PubMed identifier	22947692
schema:publication	J Virol Methods
resource representing a document's body	covid:b2cf96174de10c8c3f143ee7ca4ac8b92778c693#body_text
is schema:about of	named entity 'detect' named entity 'REVERSE TRANSCRIPTION' named entity 'DIAGNOSTIC ASSAY' named entity 'GENOTYPES' named entity 'HIGHLY' named entity 'POSITIVE' named entity 'REAL-TIME RT-PCR' named entity 'VIRAL' named entity 'HIGHLY SENSITIVE' named entity 'BVDV' named entity 'SAMPLES' named entity 'SPECIFIC' named entity 'DETECTING' named entity 'DETECTION' named entity 'BOVINE VIRAL DIARRHEA VIRUS' named entity 'METHOD' named entity 'RT-LAMP ASSAY' named entity 'ASSAY' named entity 'SWABS' named entity 'SENSITIVE' named entity 'RAPID' named entity 'BOVINE' named entity 'ABILITY' named entity 'COPIES' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'TESTING' named entity 'FOUND' named entity 'EVALUATED' named entity 'USED' named entity 'TAKEN' named entity 'FECAL' named entity 'LAMP' named entity 'REVERSE TRANSCRIPTION' named entity 'BOVINE VIRAL DIARRHEA' named entity 'CROSS-REACTION' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'OBSERVED' named entity 'CAPABLE' named entity 'TO DETECT' named entity 'CLINICAL' named entity 'RAPID' named entity 'VIRUSES' named entity 'RNA' named entity 'DIRECT' covid:arg/b2cf96174de10c8c3f143ee7ca4ac8b92778c693 named entity 'highly' named entity 'bovine' named entity 'ability' named entity 'sensitive' named entity 'highly' named entity 'fecal' named entity 'specific' named entity 'viruses' named entity 'assay' named entity 'assay' named entity 'assay' named entity 'BVDV' named entity 'assay' named entity 'BVDV' named entity 'RNA' named entity 'Primer' named entity 'BVDV' named entity 'BVDV' named entity 'Pestivirus' named entity 'bovine coronavirus' named entity 'cauliflower' named entity 'Beijing' named entity 'RT-PCR' named entity 'incubation' named entity 'fluorescent dye' named entity 'BVDV' named entity 'betaine' named entity 'DNA fragments'

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