About: A novel assay for detecting canine parvovirus using a quartz crystal microbalance biosensor

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About: A novel assay for detecting canine parvovirus using a quartz crystal microbalance biosensor Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A novel assay for detecting canine parvovirus using a quartz crystal microbalance biosensor
Creator	An, Dong-Jun Lim, Seong-In Cho, In-Soo Choi, Sarah Kim, Yong Park, Eun-Hye
Source	Elsevier; Medline; PMC
abstract	Rapid and accurate diagnosis is crucial to reduce both the shedding and clinical signs of canine parvovirus (CPV). The quartz crystal microbalance (QCM) is a new tool for measuring frequency changes associated with antigen–antibody interactions. In this study, the QCM biosensor and ProLinker™ B were used to rapidly diagnosis CPV infection. ProLinker™ B enables antibodies to be attached to a gold-coated quartz surface in a regular pattern and in the correct orientation for antigen binding. Receiver operating characteristics (ROC) curves were used to set a cut-off value using reference CPVs (two groups: one CPV-positive and one CPV-negative). The ROC curves overlapped and the point of intersection was used as the cut-off value. A QCM biosensor with a cut-off value of −205 Hz showed 95.4% (104/109) sensitivity and 98.0% (149/152) specificity when used to test 261 field fecal samples compared to PCR. In conclusion, the QCM biosensor described herein is eminently suitable for the rapid diagnosis of CPV infection with high sensitivity and specificity. Therefore, it is a promising analytical tool that will be useful for clinical diagnosis, which requires rapid and reliable analyses.
has issue date	2015-03-23(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2015.03.015
bibo:pmid	25813597
has license	no-cc
sha1sum (hex)	b4028cf2aed4166e54dd30db86f37eddb14caa3a
schema:url	https://doi.org/10.1016/j.jviromet.2015.03.015
resource representing a document's title	A novel assay for detecting canine parvovirus using a quartz crystal microbalance biosensor
has PubMed Central identifier	PMC7119597
has PubMed identifier	25813597
schema:publication	J Virol Methods
resource representing a document's body	covid:b4028cf2aed4166e54dd30db86f37eddb14caa3a#body_text
is schema:about of	named entity 'regular' named entity 'analyses' named entity 'biosensor' named entity 'field' named entity 'Therefore' named entity 'diagnosis' named entity 'QCM' named entity 'diagnosis' named entity 'frequency' named entity 'rapid' named entity 'ASSAY' named entity 'OVERLAPPED' named entity 'RAPID DIAGNOSIS' named entity 'FIELD' named entity 'ANTIGEN BINDING' named entity 'INFECTION' named entity 'CORRECT' named entity 'SENSITIVITY' named entity 'GOLD' named entity 'SPECIFICITY' named entity 'ASSOCIATED WITH' named entity 'REGULAR' named entity 'GROUPS' named entity 'ORIENTATION' named entity 'SURFACE' named entity 'QUARTZ' named entity 'USED' named entity 'NEGATIVE' named entity 'INTERSECTION' named entity 'REFERENCE' named entity 'SAMPLES' named entity 'TO REDUCE' named entity 'FECAL' named entity 'IS A' named entity 'CANINE PARVOVIRUS' named entity 'RAPIDLY' named entity 'RELIABLE' named entity 'RECEIVER' named entity 'PCR' covid:arg/b4028cf2aed4166e54dd30db86f37eddb14caa3a named entity 'ATTACHED TO' named entity 'CLINICAL DIAGNOSIS' named entity 'USING' named entity 'ENABLES' named entity 'CONCLUSION' named entity 'CUT-OFF VALUE' named entity 'POSITIVE' named entity 'ROC' named entity 'ANALYTICAL' named entity '28149' named entity 'BIOSENSOR' named entity 'FREQUENCY' named entity 'OPERATING' named entity 'CHARACTERISTICS' named entity 'ANTIGEN' named entity 'DESCRIBED' named entity 'CPV' named entity 'BIOSENSOR' named entity 'DETECTING' named entity 'QUARTZ CRYSTAL MICROBALANCE' named entity 'STUDY' named entity 'POINT' named entity 'RAPID' named entity 'USING' named entity 'MEASURING' named entity 'SET' named entity 'USEFUL' named entity 'SHEDDING' named entity 'COATED' named entity 'THE CUT' named entity 'TO TEST' named entity 'SUITABLE' named entity 'ANTIBODY INTERACTIONS' named entity 'COMPARED' named entity 'QUARTZ CRYSTAL MICROBALANCE' named entity 'CHANGES'

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