About: Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection

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About: Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection
Creator	Carney, Sharon Bonine, Lucas Goldemberg, Daniel Siqueira, Marilda Bragança, Ronaldo Checon, Rita Júnior, Martins Spano, Liliana
Source	Medline; PMC
abstract	Viruses are the major contributors to the morbidity and mortality of upper and lower acute respiratory infections (ARIs) for all age groups. The aim of this study was to determine the frequencies for a large range of respiratory viruses using a sensitive molecular detection technique in specimens from outpatients of all ages with ARIs. Nasopharyngeal aspirates were obtained from 162 individuals between August 2007-August 2009. Twenty-three pathogenic respiratory agents, 18 respiratory viruses and five bacteria were investigated using multiplex real-time reverse transcriptase polymerase chain reaction (RT-PCR) and indirect immunofluorescence assay (IIF). Through IIF, 33 (20.4%) specimens with respiratory virus were recognised, with influenza virus representing over half of the positive samples. Through a multiplex real-time RT-PCR assay, 88 (54.3%) positive samples were detected; the most prevalent respiratory viral pathogens were influenza, human rhinovirus and respiratory syncytial virus (RSV). Six cases of viral co-detection were observed, mainly involving RSV. The use of multiplex real-time RT-PCR increased the viral detection by 33.9% and revealed a larger number of respiratory viruses implicated in ARI cases, including the most recently described respiratory viruses [human bocavirus, human metapneumovirus, influenza A (H1N1) pdm09 virus, human coronavirus (HCoV) NL63 and HCoV HKU1].
has issue date	2014-09-01(xsd:dateTime)
bibo:doi	10.1590/0074-0276140046
bibo:pmid	25317699
has license	cc-by-nc
sha1sum (hex)	b439deefbf5429abf9b27dc5bed6bee148c3dfa5
schema:url	https://doi.org/10.1590/0074-0276140046
resource representing a document's title	Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection
has PubMed Central identifier	PMC4238762
has PubMed identifier	25317699
schema:publication	Mem Inst Oswaldo Cruz
resource representing a document's body	covid:b439deefbf5429abf9b27dc5bed6bee148c3dfa5#body_text
is schema:about of	named entity 'NASOPHARYNGEAL' named entity 'RANGE' named entity 'INFLUENZA A' named entity 'OBSERVED' named entity 'TECHNIQUE' named entity 'PATHOGENIC' named entity 'RESPIRATORY VIRUS' named entity 'HUMAN RHINOVIRUS' named entity 'REAL-TIME RT-PCR' named entity 'VIRAL DETECTION' named entity 'RESPIRATORY SYNCYTIAL VIRUS' named entity 'NL63' named entity 'PREVALENT' named entity 'MULTIPLEX' named entity 'RT-PCR ASSAY' named entity 'SAMPLES' named entity 'PCR' named entity 'ACUTE RESPIRATORY INFECTIONS' named entity 'MAJOR' named entity 'STUDY' named entity 'AGE GROUPS' named entity 'ARI' named entity 'FREQUENCIES' named entity 'RESPIRATORY VIRUSES' named entity 'REAL-TIME' named entity 'OUTPATIENTS' named entity '33.9' named entity 'RSV' named entity 'LARGE' named entity 'REVEALED' named entity 'DETERMINE' named entity 'NUMBER OF' named entity 'TWENTY-THREE' named entity 'OBTAINED' named entity 'RECENTLY' named entity 'ALL AGES' named entity 'REAL-TIME POLYMERASE CHAIN REACTION' named entity 'DETECTION' named entity 'ACUTE RESPIRATORY INFECTION' named entity 'IMMUNOFLUORESCENCE ASSAY' named entity 'INDIRECT IMMUNOFLUORESCENCE' named entity 'CASES' named entity 'RESPIRATORY VIRUSES' named entity 'INCREASED' named entity 'OUTPATIENTS' named entity 'RESPIRATORY' named entity 'INFLUENZA' named entity 'INVOLVING' named entity 'ASPIRATES' named entity 'LOWER' named entity 'SENSITIVE' named entity 'HUMAN METAPNEUMOVIRUS' named entity 'MORBIDITY AND MORTALITY' named entity 'AIM' named entity 'INCLUDING' named entity 'REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION' named entity 'HUMAN CORONAVIRUS' named entity 'UPPER' named entity 'INVESTIGATED' named entity 'VIRAL' named entity 'LARGER' named entity 'HALF' named entity 'DESCRIBED' named entity 'BOCAVIRUS' named entity 'INDIVIDUALS' named entity 'VIRUS' named entity 'AUGUST' named entity 'MOLECULAR' named entity '162' named entity 'SPECIMENS' named entity 'USE OF' named entity 'DETECTION' named entity 'DETECTED' named entity 'USING'

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