About: Analysis of SARS-CoV E protein ion channel activity by tuning the protein and lipid charge

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Analysis of SARS-CoV E protein ion channel activity by tuning the protein and lipid charge
Creator	Enjuanes, Luis Alcaraz, Antonio Verdiá-Báguena, Carmina Aguilella, Vicente Dediego, Marta Nieto-Torres, Jose
Source	Elsevier; Medline; PMC
abstract	Abstract A partial characterization of the ion channels formed by the SARS coronavirus (CoV) envelope (E) protein was previously reported (C. Verdiá-Báguena et al., 2012 [12]). Here, we provide new significant insights on the involvement of lipids in the structure and function of the CoV E protein channel on the basis of three series of experiments. First, reversal potential measurements over a wide range of pH allow the dissection of the contributions to channel selectivity coming from ionizable residues of the protein transmembrane domain and also from the negatively charged groups of diphytanoyl phosphatidylserine (DPhPS) lipid. The corresponding effective pKas are consistent with the model pKas of the acidic residue candidates for titration. Second, the change of channel conductance with salt concentration reveals two distinct regimes (Donnan-controlled electrodiffusion and bulk-like electrodiffusion) fully compatible with the outcomes of selectivity experiments. Third, by measuring channel conductance in mixtures of neutral diphytanoyl phosphatidylcholine (DPhPC) lipids and negatively charged DPhPS lipids in low and high salt concentrations we conclude that the protein–lipid conformation in the channel is likely the same in charged and neutral lipids. Overall, the whole set of experiments supports the proteolipidic structure of SARS-CoV E channels and explains the large difference in channel conductance observed between neutral and charged membranes.
has issue date	2013-09-30(xsd:dateTime)
bibo:doi	10.1016/j.bbamem.2013.05.008
bibo:pmid	23688394
has license	els-covid
sha1sum (hex)	b45941ca51d3a1efa379dcb5b0c56b23ed0729a6
schema:url	https://doi.org/10.1016/j.bbamem.2013.05.008
resource representing a document's title	Analysis of SARS-CoV E protein ion channel activity by tuning the protein and lipid charge
has PubMed Central identifier	PMC3715572
has PubMed identifier	23688394
schema:publication	Biochimica et Biophysica Acta (BBA) - Biomembranes
resource representing a document's body	covid:b45941ca51d3a1efa379dcb5b0c56b23ed0729a6#body_text
is schema:about of	named entity 'structure' named entity 'neutral' named entity 'electrodiffusion' named entity 'channel conductance' named entity 'Bulk' named entity 'large' named entity 'Analysis' named entity 'protein' named entity 'lipid' named entity 'PROTEIN ' named entity 'RANGE' named entity 'SOLUTION' named entity 'E PROTEIN' named entity 'LIKE' named entity 'GROUPS' named entity 'WIDE' named entity 'FORMED' named entity 'DISSECTION' named entity 'STRUCTURE' named entity 'A1-B' named entity 'PARTIAL' named entity 'CONSISTENT WITH' named entity 'LIPID CONFORMATION' named entity 'TO CHANNEL' named entity 'CONDUCTIVITY' named entity 'CONDUCTANCE' named entity 'SARS CORONAVIRUS' named entity 'LIPIDS' named entity 'HIGH' named entity 'PROTEIN' named entity 'MIXTURES' named entity 'DISTINCT' named entity 'C O' named entity 'FUNCTION' named entity 'DIFFERENCE' named entity 'INSIGHTS' named entity 'ANALYSIS' named entity 'PROTEIN ' named entity 'SARS-COV' named entity 'COMPATIBLE WITH' named entity 'HERE' named entity 'E PROTEIN' named entity 'ION CHANNEL ACTIVITY' named entity 'LIPID' named entity 'CHARGE' named entity 'CONCLUDE' named entity 'L H' named entity 'FULLY' named entity 'N A' named entity 'EFFECTIVE' named entity 'THE CHANGE' named entity 'MEMBRANES' named entity 'LOW' named entity 'ALLOW' named entity 'ION CHANNELS' named entity 'SARS-COV' named entity 'OUTCOMES' named entity 'CHARGED' named entity 'I E' named entity '28S' named entity 'IONIZABLE' named entity 'REVERSAL POTENTIAL' named entity 'INVOLVEMENT' named entity 'TITRATION' named entity 'L S' named entity 'SELECTIVITY' named entity 'BULK' named entity 'CHANNEL' named entity 'PROVIDE' named entity 'CHARACTERIZATION' named entity 'COMING' named entity 'ENVELOPE' named entity 'SIGNIFICANT' named entity 'SALT' named entity 'MODEL' named entity 'A T'

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