About: Development of a novel DNA-launched dengue virus type 2 infectious clone assembled in a bacterial artificial chromosome

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About: Development of a novel DNA-launched dengue virus type 2 infectious clone assembled in a bacterial artificial chromosome Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of a novel DNA-launched dengue virus type 2 infectious clone assembled in a bacterial artificial chromosome
Creator	Enjuanes, Luis Almazán, Fernando Gallego-Gomez, Juan Usme-Ciro, Jose Lopera, Jaime
topic	covid:b4991ef774eb4e7811f8aae5ac768cb335206183#this
Source	Elsevier; Medline; PMC
abstract	Abstract Major progress in Dengue virus (DENV) biology has resulted from the use of infectious clones obtained through reverse genetics. The construction of these clones is commonly based on high- or low-copy number plasmids, yeast artificial chromosomes, yeast-Escherichia coli shuttle vectors, and bacterial artificial chromosomes (BACs). Prokaryotic promoters have consistently been used for the transcription of these clones. The goal of this study was to develop a novel DENV infectious clone in a BAC under the control of the cytomegalovirus immediate-early promoter and to generate a virus with the fusion envelope-green fluorescent protein in an attempt to track virus infection. The transfection of Vero cells with a plasmid encoding the DENV infectious clone facilitated the recovery of infectious particles that increased in titer after serial passages in C6/36 cells. The plaque size and syncytia phenotypes of the recombinant virus were similar to those of the parental virus. Despite the observation of autonomous replication and the detection of low levels of viral genome after two passages, the insertion of green fluorescent protein and Renilla luciferase reporter genes negatively impacted virus rescue. To the best of our knowledge, this is the first study using a DENV infectious clone under the control of the cytomegalovirus promoter to facilitate the recovery of recombinant viruses without the need for in vitro transcription. This novel molecular clone will be useful for establishing the molecular basis of replication, assembly, and pathogenesis, evaluating potential antiviral drugs, and the development of vaccine candidates for attenuated recombinant viruses.
has issue date	2014-02-13(xsd:dateTime)
bibo:doi	10.1016/j.virusres.2013.12.001
bibo:pmid	24342140
has license	els-covid
sha1sum (hex)	b4991ef774eb4e7811f8aae5ac768cb335206183
schema:url	https://doi.org/10.1016/j.virusres.2013.12.001
resource representing a document's title	Development of a novel DNA-launched dengue virus type 2 infectious clone assembled in a bacterial artificial chromosome
has PubMed Central identifier	PMC7114509
has PubMed identifier	24342140
schema:publication	Virus Research
resource representing a document's body	covid:b4991ef774eb4e7811f8aae5ac768cb335206183#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/b4991ef774eb4e7811f8aae5ac768cb335206183
is schema:about of	named entity 'infection' named entity 'evaluating' named entity 'replication' named entity 'bacterial artificial chromosomes' named entity 'clone' named entity 'DENV' named entity 'reverse genetics' named entity 'pathogenesis' named entity 'genes' named entity 'Despite' named entity 'bacterial artificial chromosome' named entity 'PLASMIDS' named entity 'MOLECULAR' named entity 'SIZE' named entity 'ANTIVIRAL DRUGS' named entity 'NOVEL' named entity 'HIGH' named entity 'USING' named entity 'ARTIFICIAL' named entity 'PLASMID' named entity 'VIRUS INFECTION' named entity 'PROMOTER' named entity 'INCREASED' named entity 'ENCODING' named entity 'DEVELOPMENT' named entity 'POTENTIAL' named entity 'RECOMBINANT' named entity 'AUTONOMOUS' named entity 'OBTAINED' named entity 'ATTEMPT' named entity 'PROGRESS' named entity 'VACCINE' named entity 'ASSEMBLY' named entity 'VIRAL GENOME' named entity 'BASED' named entity 'LEVELS' named entity 'HAVE' named entity 'EVALUATING' named entity 'OBSERVATION' named entity 'GENERATE' named entity 'STUDY' named entity 'PROTEIN ' named entity 'SYNCYTIA' named entity 'MOLECULAR BASIS' named entity 'IN VITRO TRANSCRIPTION' named entity 'REPLICATION' named entity 'IMPACTED' covid:arg/b4991ef774eb4e7811f8aae5ac768cb335206183 named entity 'RENILLA LUCIFERASE' named entity 'PROKARYOTIC' named entity 'SIMILAR' named entity 'VERO CELLS' named entity 'VIRUSES' named entity 'KNOWLEDGE' named entity 'CONTROL' named entity 'DENGUE VIRUS' named entity 'VIRUS' named entity 'RESCUE' named entity 'TRANSFECTION' named entity 'TRANSCRIPTION' named entity 'REPORTER GENES' named entity 'ESCHERICHIA COLI' named entity 'PROMOTERS' named entity 'MAJOR' named entity 'USE OF' named entity 'DEVELOPMENT' named entity 'DENGUE VIRUS TYPE 2' named entity 'DNA' named entity 'BACTERIAL ARTIFICIAL CHROMOSOME' named entity 'BIOLOGY' named entity 'TITER' named entity 'PATHOGENESIS' named entity 'PLAQUE' named entity 'NEED FOR' named entity 'BAC'

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