About: Design of recombinant protein-based SARS-CoV entry inhibitors targeting the heptad-repeat regions of the spike protein S2 domain

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About: Design of recombinant protein-based SARS-CoV entry inhibitors targeting the heptad-repeat regions of the spike protein S2 domain Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Design of recombinant protein-based SARS-CoV entry inhibitors targeting the heptad-repeat regions of the spike protein S2 domain
Creator	Tien, Po Gao, George Zhu, Jieqing Ni, Ling Yan, Meng Zhang, Junjie
Source	Elsevier; Medline; PMC
abstract	Abstract Entry of SARS-CoV into a target cell is initiated by binding of the S1 domain of spike protein to a receptor, followed by conformational changes of the spike protein S2 domain, resulting in the formation of a six-helix bundle by the heptad-repeat (HR1 and HR2) regions. Our previous studies have demonstrated that peptides derived from HR2 region could inhibit SARS-CoV entry. However, synthesis of these peptides is at high cost. In this study, we designed two recombinant proteins, one containing two HR1 and one HR2 peptides (denoted HR121), and the other consisting of two HR2 and one HR1 peptides (designated HR212). These two proteins could be easily purified with the low cost of production, exhibiting high stability and potent inhibitory activity on entry of the HIV/SARS pseudoviruses with IC50 values of 4.13 and 0.95μM, respectively. These features suggest that HR121 and HR212 can serve as potent inhibitors of SARS-CoV entry.
has issue date	2005-04-29(xsd:dateTime)
bibo:doi	10.1016/j.bbrc.2005.02.117
bibo:pmid	15781229
has license	els-covid
sha1sum (hex)	b4a7540cc6819370dd10f1363facc7fcde3bad45
schema:url	https://doi.org/10.1016/j.bbrc.2005.02.117
resource representing a document's title	Design of recombinant protein-based SARS-CoV entry inhibitors targeting the heptad-repeat regions of the spike protein S2 domain
has PubMed Central identifier	PMC7092889
has PubMed identifier	15781229
schema:publication	Biochemical and Biophysical Research Communications
resource representing a document's body	covid:b4a7540cc6819370dd10f1363facc7fcde3bad45#body_text
is schema:about of	named entity 'target cell' named entity 'denoted' named entity 'peptides' covid:arg/b4a7540cc6819370dd10f1363facc7fcde3bad45 named entity 'HR2' named entity 'These' named entity 'study' named entity 'domain' named entity 'HR2' named entity 'SARS-CoV' named entity 'cost' named entity 'SARS-CoV' named entity 'inhibitory' named entity 'features' named entity 'stability' named entity 'HR2' named entity 'SARS' named entity 'SARS-CoV' named entity 'HR2' named entity 'spike protein' named entity 'luciferase' named entity 'supernatants' named entity 'membrane fusion' named entity 'helix bundle' named entity 'helix core' named entity 'interact' named entity 'Menangle virus' named entity 'membrane fusion' named entity 'HIV-1' named entity 'thermostable' named entity 'transmembrane' named entity 'HIV' named entity 'SDS-PAGE' named entity 'pull-down assay' named entity 'retrovirus' named entity 'DMSO' named entity '37°C' named entity 'gp41' named entity 'fusion proteins' named entity 'HR2' named entity '1.02' named entity 'dimer' named entity 'amino acids' named entity 'N35' named entity 'HR2' named entity 'carboxy terminus' named entity 'peptides' named entity 'peptides' named entity 'fusion proteins' named entity 'virus' named entity 'molecular weight' named entity 'ultrafiltration' named entity 'glutathione' named entity 'trimer' named entity 'elution' named entity 'HR2' named entity 'conformation' named entity 'fusion proteins' named entity 'HR2' named entity 'inhibitory activity' named entity 'trimer' named entity 'molecular weights' named entity 'eluted' named entity 'glutathione' named entity 'codon' named entity 'HR2' named entity 'protein' named entity 'physiological' named entity 'N44' named entity 'fusion core' named entity 'amino acids' named entity 'supernatants' named entity 'protein expression' named entity 'pET' named entity 'HR2' named entity 'fusion protein'

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