About: Room-temperature-storable PCR Mixes for SARS-CoV-2 Detection

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Room-temperature-storable PCR Mixes for SARS-CoV-2 Detection
Creator	Zhang, Jun Xia, Ningshao Wang, Jin Zhang, J Wang, Y Li, T Chen, Z Wang, J Xu, J Yang, K Zhang, D Zhang, S Ge, Shengxiang Li, Tingdong Su, X Chen, Zhongfu Su, Xiaosong Wang, Yingbin Yang, Kunyu Xu, Jiasu Zhang, Shiyin Zhang, Dongxu Zhong, Zecheng Zhong, Z Ge, S
Source	Elsevier; Medline; PMC
abstract	OBJECTIVES: A novel coronavirus (severe acute respiratory syndrome coronavirus 2, SARS-CoV-2) emerged in late 2019, causing an outbreak of pneumonia [coronavirus disease 2019 (COVID-19)] globally. Although the use of ready-made reaction mixes can enable more rapid PCR-based diagnosis of COVID-19, the need to transport and store these mixes at low temperatures presents challenges to already overburdened logistics networks. METHODS: Here, we present an optimized freeze-drying procedure that allows SARS-CoV-2 PCR mixes to be transported and stored at ambient temperatures, without loss of activity. Additive-supplemented PCR mixes were freeze-dried. The residual moisture of the freeze-dried PCR mixes was measured by Karl-Fischer titration. RESULTS: We found that freeze-dried PCR mixes with ∼1.2% residual moisture are optimal for storage, transport, and reconstitution. The sensitivity, specificity, and repeatability of the freeze-dried reagents were similar to those of freshly prepared, wet reagents. The freeze-dried mixes retained activity at room temperature (18∼25℃) for 28 days, and for 14 and 10 days when stored at 37℃ and 56℃, respectively. CONCLUSION: The uptake of this approach will ease logistical challenges faced by transport networks and make more cold storage space available at diagnosis and hospital laboratories.
has issue date	2020-06-24(xsd:dateTime)
bibo:doi	10.1016/j.clinbiochem.2020.06.013
bibo:pmid	32592724
has license	no-cc
sha1sum (hex)	b5a051e5722ac383ea56dab7fda889593a9b6403
schema:url	https://doi.org/10.1016/j.clinbiochem.2020.06.013
resource representing a document's title	Room-temperature-storable PCR Mixes for SARS-CoV-2 Detection
has PubMed Central identifier	PMC7313492
has PubMed identifier	32592724
schema:publication	Clin Biochem
resource representing a document's body	covid:b5a051e5722ac383ea56dab7fda889593a9b6403#body_text
is schema:about of	named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'PCR' named entity 'REACTION' named entity 'LOGISTICS' named entity 'CHALLENGES' named entity 'BASED' named entity 'TO TRANSPORT' named entity 'LATE' named entity 'NETWORKS' named entity 'causing' named entity 'mixes' named entity 'Although' named entity 'COVID-19' named entity 'PCR' named entity 'freeze-drying' named entity 'rRT-PCR' named entity '20 weeks' named entity 'lyophilization' named entity 'serial dilution' named entity 'low temperature' named entity 'PCR' named entity 'PCR' named entity 'freeze-dried' named entity 'lyophilization' named entity 'reverse transcriptase' named entity 'freeze-dried' named entity 'avian influenza virus' named entity 'RNA' named entity 'transport logistics' named entity 'amplification efficiency' named entity 'freeze-dried' named entity 'virus' named entity 'critical process parameters' named entity 'PCR' named entity 'PCR' named entity 'PCR' named entity 'titration' named entity 'freeze-dried' named entity 'public health' named entity 'fluorescence' named entity 'primers' named entity 'Sigma-Aldrich' named entity 'fluorescence' named entity 'PCR' named entity 'shelf-life' named entity 'lyophilized' named entity 'freeze-dried' named entity '28 days' named entity 'fluorescence' named entity 'primers' named entity 'lyophilize' named entity 'titrator' named entity 'freeze-dried' named entity 'Karl-Fischer titration' named entity 'diffusion' named entity 'fluorescence'

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