About: A new Sephadex™-based method for removing microbicidal and cytotoxic residues when testing antiseptics against viruses: Experiments with a human coronavirus as a model

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About: A new Sephadex™-based method for removing microbicidal and cytotoxic residues when testing antiseptics against viruses: Experiments with a human coronavirus as a model Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A new Sephadex™-based method for removing microbicidal and cytotoxic residues when testing antiseptics against viruses: Experiments with a human coronavirus as a model
Creator	Finance, Chantal Fontanay, Stéphane Geller, Chloé Duval, Raphaël
Source	Elsevier; Medline; PMC
abstract	Abstract The relative lack of efficient methods for evaluating antiseptic antiviral activity, together with weaknesses in the existing European Standard (i.e. NF EN 14476+A1), underlines the need to seek a new method which could allow a more precise evaluation of the antiseptic antiviral activity of chemical agents. This protocol is based on an original gel-based filtration method, using “in-house” G-25 and G-10 Sephadex™ columns. This method allows the neutralization of both the activity and the cytotoxicity of a large range of molecules, according to their molecular size, in only 1min. The viral model used was the human coronavirus (HCoV) 229E chosen for (i) its increasing medical interest, (ii) its potential resistance and (iii) its representing enveloped viruses mentioned in the European Standard. First, the protocol was validated and it was demonstrated that it was fully operational for evaluating antiviral antiseptic potentiality and useful to screen potentially antiseptic molecules. Second, chlorhexidine (CHX) and hexamidine (HXM) were assessed for their potential anti-HCoV 229E antiseptic activities. It was demonstrated clearly that (i) HXM had no activity on the HCoV 229E and (ii) CHX showed a moderate anti-HCoV 229E activity but insufficient to be antiseptic.
has issue date	2009-08-31(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2009.03.023
bibo:pmid	19490977
has license	els-covid
sha1sum (hex)	b66927ad32d3e265b777f241d353474af4416512
schema:url	https://doi.org/10.1016/j.jviromet.2009.03.023
resource representing a document's title	A new Sephadex™-based method for removing microbicidal and cytotoxic residues when testing antiseptics against viruses: Experiments with a human coronavirus as a model
has PubMed Central identifier	PMC7112946
has PubMed identifier	19490977
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:b66927ad32d3e265b777f241d353474af4416512#body_text
is schema:about of	named entity 'CYTOTOXIC' named entity 'precise' named entity 'microbicidal' named entity 'ANTISEPTIC' named entity 'ALLOW' named entity 'RELATIVE' named entity 'VIRUSES' named entity 'REMOVING' named entity 'EVALUATION' named entity 'NEW' named entity 'METHODS' named entity 'WEAKNESSES' named entity 'EFFICIENT' named entity 'EUROPEAN' named entity 'BASED' named entity 'TESTING' named entity 'MODEL' named entity 'ANTISEPTICS' named entity 'SEPHADEX' named entity 'NEED' named entity 'CHEMICAL AGENTS' named entity 'ANTIVIRAL ACTIVITY' named entity 'LACK' named entity 'EVALUATING' named entity 'PRECISE' named entity 'STANDARD' named entity '28I' named entity 'METHOD' named entity 'NEW' named entity 'EXPERIMENTS' named entity 'HUMAN CORONAVIRUS' named entity 'METHOD' covid:arg/b66927ad32d3e265b777f241d353474af4416512 named entity 'European Standard' named entity 'evaluating' named entity 'viruses' named entity 'coronavirus' named entity 'chemical agents' named entity 'Sephadex' named entity 'airborne transmission' named entity 'ophthalmologic' named entity 'spectrophotometry' named entity 'virucidal' named entity 'spectrophotometric' named entity 'centrifugation' named entity 'virus' named entity 'infectivity' named entity 'virus' named entity 'pathogenesis' named entity 'CHX' named entity 'Sephadex' named entity 'Sephadex' named entity 'cytotoxicity' named entity '229E' named entity 'sterilization' named entity 'acetic acid' named entity 'Sephadex' named entity 'viruses' named entity 'Sephadex' named entity 'viral infections' named entity 'Lille' named entity 'virus' named entity 'Giemsa' named entity 'Sephadex' named entity 'centrifugation' named entity 'mitochondrial' named entity 'G-10' named entity 'antiseptic' named entity 'Sephadex' named entity '229E' named entity 'molecular size' named entity 'Sephadex' named entity 'antiseptic' named entity 'viruses' named entity 'viral titers' named entity 'distilled water' named entity 'Sephadex' named entity 'viral titers'

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