About: Junín virus induces autophagy in human A549 cells

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Junín virus induces autophagy in human A549 cells
Creator	Romanowski, Víctor Ure, Agustín Arríasid, Paula Gó Mez, Ricardo Laura, Maria Perez Vidakovicsid, A
Source	PMC
abstract	Autophagy, a highly regulated degradative process that promotes cellular homeostasis, is increasingly recognised as a fundamental component of the cellular response against viral infection. In this study, we investigated the role of autophagy during Junín virus (JUNV) multiplication using human A549 cells. We found that JUNV infection induces an increment of the LC3-II/LC3-I ratio, an accumulation of punctate pattern in RFP-LC3-transfected cells and the colocalisation of viral nucleoprotein and LC3 protein, suggesting autophagosome formation. JUNV infection also induced the degradation of the autophagy receptor p62, suggesting that complete autophagic flux was triggered. In addition, we showed that inhibition of autophagy with bafilomycin A1 or 3-methyladenine significantly reduces viral multiplication. Moreover, viral yield was increased when autophagy was induced using rapamycin. Furthermore, JUNV infection induced the colocalisation of p62, ATG16, RAB5, RAB7A and LAMP1 with the autophagosomal LC3 protein. That suggests that phagosomes undergo the maturation process during viral infection. Finally, we demonstrated that siRNA experiments targeting essential autophagy genes (ATG5, ATG7 and Beclin 1) reduce viral protein synthesis and viral yield. Overall, our results indicate that JUNV activates host autophagy machinery enhancing its multiplication.
has issue date	2019-06-19(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0218730
bibo:pmid	31216340
has license	cc-by
sha1sum (hex)	b66c037ebfee8059dd47d2614cf5d55618d8260f
schema:url	https://doi.org/10.1371/journal.pone.0218730
resource representing a document's title	Junín virus induces autophagy in human A549 cells
has PubMed Central identifier	PMC6583977
has PubMed identifier	31216340
schema:publication	PLoS One
resource representing a document's body	covid:b66c037ebfee8059dd47d2614cf5d55618d8260f#body_text
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