About: Comparison of primer-probe sets among different master mixes for laboratory screening of Severe Acute Respiratory Syndrome

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About: Comparison of primer-probe sets among different master mixes for laboratory screening of Severe Acute Respiratory Syndrome - Coronavirus 2 (SARS-CoV-2) Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Comparison of primer-probe sets among different master mixes for laboratory screening of Severe Acute Respiratory Syndrome - Coronavirus 2 (SARS-CoV-2)
Creator	Thi, Nguyen Duc, Nguyen Hoang, Nguyen Linh, Hoang Thang, Cao Thao, Thanh Cuong, Hoang Trong, Phan Trung Hieu, Nguyen
Source	MedRxiv
abstract	Background: The shortage of chemical reagents for severe acute respiratory syndrome - coronavirus 2 (SARS-CoV-2) diagnosis and the surge of SARS-CoV-2 cases, especially in limited-resource settings. Therefore, the combination of an optimal assay kit is necessary. Methods: We compared the ability to screen SARS-CoV-2 among three primer-probe sets added into two different master mixes (Invitrogen SuperScript III One-Step RT-PCR, and LightCycler Multiplex RNA Virus Master). Results: The assay with TIB-Molbiol, IDT, and Phu Sa sets for LightCycler Multiplex RNA Virus Master or Invitrogen SuperScript III One-Step RT-PCR showed positive results from a single reaction of triplicate in the three days of 4.8 copies per reaction, with R-square and amplification efficiently (AE) were 0.97 and ranged from 107 to 108%, respectively. Conclusions: Our findings indicated TIB-Molbiol, IDT, and Phu Sa primer-probe sets could be beneficial for the laboratory screening of SARS-CoV-2 by Realtime RT-PCR assay of E gene. There is a need for considering the combination of these reagent sets as a new strategy to increase testing capacity for screening programs for COVID-19.
has issue date	2020-06-22(xsd:dateTime)
bibo:doi	10.1101/2020.06.20.20136242
has license	medrxiv
sha1sum (hex)	b7321850dbe04c3114e2185760fef2784777c6ef
schema:url	https://doi.org/10.1101/2020.06.20.20136242
resource representing a document's title	Comparison of primer-probe sets among different master mixes for laboratory screening of Severe Acute Respiratory Syndrome - Coronavirus 2 (SARS-CoV-2)
resource representing a document's body	covid:b7321850dbe04c3114e2185760fef2784777c6ef#body_text
is schema:about of	named entity 'short' named entity 'screening' named entity 'PERMISSION' named entity 'All rights reserved' named entity 'laboratory' named entity 'master mixes' named entity 'gene' named entity 'medRxiv' named entity 'RNA Virus' named entity 'coronavirus vaccine' named entity 'R-square' named entity 'peer review' named entity 'infection' named entity '13.0' named entity 'virus' named entity 'RNA Virus' named entity 'SARS-CoV-2' named entity 'standard deviation' named entity 'SARS-CoV-2' named entity 'gene' named entity 'viral load' named entity 'Skokie' named entity 'SARS-CoV-2' named entity 'RT-PCR' named entity 'medRxiv' named entity 'viral RNA' named entity 'SARS-CoV-2' named entity 'R-square' named entity 'peer review' named entity 'RT-PCR' named entity 'reagent' named entity 'COVID-19' named entity 'Institutional Review Board' named entity 'Viral' named entity 'peer review' named entity 'SARS-CoV-2' named entity 'RT-PCR' named entity 'RNA' named entity 'SARS-CoV-2' named entity 'prevention and control' named entity 'Qiagen' named entity 'Berlin' named entity 'master mixes' named entity 'chemical reagents' named entity 'master mixes' named entity 'ideal solution' named entity 'Vero E6' named entity 'master mixes' named entity 'SARS-CoV-2' named entity 'standard curve' named entity 'Germany' named entity 'peer review' named entity 'social distancing' named entity 'Hilden' named entity 'RNA' named entity 'RNA Virus' named entity 'screening programs' named entity 'infection' named entity 'reagent' named entity 'RT-PCR' named entity 'social distancing' named entity 'SARS-CoV-2' named entity 'RT-PCR' named entity 'RNA' named entity 'statistical analysis' named entity 'culture medium' named entity 'COVID-19' named entity 'peer review' named entity '2, 3' named entity 'Odense' named entity 'SARS-CoV-2' named entity 'COVID' named entity 'SARS-CoV-2' named entity 'COVID' named entity 'regression analysis' named entity 'virus' named entity 'SARS-CoV-2'

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