About: A Robust, Safe and Scalable Magnetic Nanoparticle Workflow for RNA Extraction of Pathogens from Clinical and Environmental Samples

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About: A Robust, Safe and Scalable Magnetic Nanoparticle Workflow for RNA Extraction of Pathogens from Clinical and Environmental Samples Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A Robust, Safe and Scalable Magnetic Nanoparticle Workflow for RNA Extraction of Pathogens from Clinical and Environmental Samples
Creator	Arabia, Saudi Hashem, Anwar Pain, Arnab Li, Mo Almontashiri, Naif Alofi, Fadwa Alsomali, Afrah Hala, Sharif Hamdan, Samir Khogeer, Asim Rachmadi, Andri Salunke, Rahul Affairs, Guard Hong, Peiying Mfarrej, Sara Ramos-Mandujano, Gerardo Xu, Jinna
Source	MedRxiv
abstract	Diagnosis and surveillance of emerging pathogens such as SARS-CoV-2 depend on nucleic acid isolation from clinical and environmental samples. Under normal circumstances, samples would be processed using commercial proprietary reagents in Biosafety 2 (BSL-2) or higher facilities. A pandemic at the scale of COVID-19 has caused a global shortage of proprietary reagents and BSL-2 laboratories to safely perform testing. Therefore, alternative solutions are urgently needed to address these challenges. We developed an open-source method called Magnetic- nanoparticle-Aided Viral RNA Isolation of Contagious Samples (MAVRICS) that is built upon reagents that are either readily available or can be synthesized in any molecular biology laboratory with basic equipment. Unlike conventional methods, MAVRICS works directly in samples inactivated in acid guanidinium thiocyanate-phenol-chloroform (e.g., TRIzol), thus allowing infectious samples to be handled safely without biocontainment facilities. Using 36 COVID-19 patient samples, 2 wastewater samples and 1 human pathogens control sample, we showed that MAVRICS rivals commercial kits in validated diagnostic tests of SARS-CoV-2, influenza viruses, and respiratory syncytial virus. MAVRICS is scalable and thus could become an enabling technology for widespread community testing and wastewater monitoring in the current and future pandemics.
has issue date	2020-06-29(xsd:dateTime)
bibo:doi	10.1101/2020.06.28.20141945
has license	medrxiv
sha1sum (hex)	b791812a7dd3f0bd53f7aabb9e8c8d2fc58ef158
schema:url	https://doi.org/10.1101/2020.06.28.20141945
resource representing a document's title	A Robust, Safe and Scalable Magnetic Nanoparticle Workflow for RNA Extraction of Pathogens from Clinical and Environmental Samples
resource representing a document's body	covid:b791812a7dd3f0bd53f7aabb9e8c8d2fc58ef158#body_text
is schema:about of	named entity 'USING' named entity 'FACILITIES' named entity 'CLINICAL' named entity 'LABORATORIES' named entity 'BIOLOGY LABORATORY' named entity 'MONITORING' named entity 'HIGHER' named entity 'COVID-19' named entity 'BIOSAFETY' named entity 'ENABLING TECHNOLOGY' named entity 'TESTING' named entity 'CHLOROFORM' covid:arg/b791812a7dd3f0bd53f7aabb9e8c8d2fc58ef158 named entity 'diagnostic tests' named entity 'biocontainment' named entity 'chloroform' named entity 'RNA' named entity 'SARS-CoV-2' named entity 'BSL-2' named entity 'control sample' named entity 'carboxyl' named entity 'medRxiv' named entity 'supernatant' named entity 'murine' named entity 'RNA isolation' named entity 'preprint' named entity 'influenza viruses' named entity 'preprint' named entity 'June 29' named entity 'lyse' named entity 'RNA' named entity 'primer' named entity 'TRIzol' named entity 'preprint' named entity 'medRxiv' named entity 'library preparation' named entity 'centrifugation' named entity 'RNA' named entity 'reverse transcription polymerase chain reaction' named entity 'reverse transcription' named entity 'rRT-PCR' named entity 'rRT-PCR' named entity 'high-throughput sequencing' named entity 'pathogens' named entity 'preprint' named entity 'Nucleic acid' named entity 'nucleic acid' named entity 'supply chain' named entity 'lysed' named entity 'virus' named entity 'RNA' named entity 'SARS-CoV-2' named entity 'CHCl3' named entity 'RNA binding' named entity 'real-time PCR' named entity 'pathogens' named entity 'medRxiv' named entity 'rRT-PCR' named entity 'preprint' named entity '3, 4' named entity 'preprint' named entity 'preprint' named entity 'viral RNA' named entity 'electronegative' named entity 'RNA isolation' named entity 'saliva samples' named entity 'RNA extraction' named entity 'preprint' named entity 'SPRI'

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