About: Middle East Respiratory Syndrome Coronavirus in Dromedaries in Ethiopia Is Antigenically Different From the Middle East Isolate EMC

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About: Middle East Respiratory Syndrome Coronavirus in Dromedaries in Ethiopia Is Antigenically Different From the Middle East Isolate EMC Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Middle East Respiratory Syndrome Coronavirus in Dromedaries in Ethiopia Is Antigenically Different From the Middle East Isolate EMC
Creator	Perlman, Stanley Matsuyama, Shutoku Nao, Naganori Shirato, Kazuya Iwata-Yoshikawa, Naoko Sentsui, Hiroshi Kamitani, Wataru Kawase, Miyuki Kawachi, Kengo Keskes Melaku, Simenew Tessema, Tesfaye Yassine, Hadi Yokota, Yasuko
Source	Medline; PMC
abstract	Middle East respiratory syndrome (MERS) is an emerging respiratory disease caused by the MERS coronavirus (MERS-CoV). MERS has been endemic to Saudi Arabia since 2012. The reservoir of MERS-CoV is the dromedary camel, suggesting that MERS is primarily a zoonotic disease. MERS-CoV is common in dromedaries throughout the Middle East, North Africa, and East Africa as evidenced by neutralizing antibodies against MERS-CoV; however, human cases have remained limited to the Middle East. To better understand the cause of this difference, the virological properties of African camel MERS-CoV were analyzed based on the spike (S) protein in Ethiopia. Nasal swabs were collected from 258 young dromedaries (≤ 2 years old) in the Afar region of Ethiopia, of which 39 were positive for MERS-CoV, as confirmed by genetic tests. All positive tests were exclusive to the Amibara woreda region. Using next-generation sequencing, two full-length genomes of Amibara isolates were successfully decoded; both isolates belonged to the C2 clade based on phylogenetic analysis of full-length and S protein sequences. Recombinant EMC isolates of MERS-CoV, in which the S protein is replaced with those of Amibara isolates, were then generated to test the roles of these proteins in viral properties. Amibara S recombinants replicated more slowly in cultured cells than in EMC S recombinants. In neutralizing assays, Amibara S recombinants were neutralized by lower concentrations of sera from both Ethiopian dromedaries and EMC isolate (wild-type)-immunized mouse sera, relative to the EMC S recombinants, indicating that viruses coated in the Amibara S protein were easier to neutralize than the EMC S protein. Neutralization experiments performed using S1/S2 chimeric recombinants of the EMC and Amibara S proteins showed that the neutralization profile was dependent on the S1 region of the S protein. These results suggest that the slower viral replication and the ease of neutralization seen in the Ethiopian MERS-CoV are due to strain-specific differences in the S protein and may account for the absence of human MERS-CoV cases in Ethiopia.
has issue date	2019-06-19(xsd:dateTime)
bibo:doi	10.3389/fmicb.2019.01326
bibo:pmid	31275264
has license	cc-by
sha1sum (hex)	b7f090aebd3ca0c64361662b87a6134c0afacb1a
schema:url	https://doi.org/10.3389/fmicb.2019.01326
resource representing a document's title	Middle East Respiratory Syndrome Coronavirus in Dromedaries in Ethiopia Is Antigenically Different From the Middle East Isolate EMC
has PubMed Central identifier	PMC6593072
has PubMed identifier	31275264
schema:publication	Front Microbiol
resource representing a document's body	covid:b7f090aebd3ca0c64361662b87a6134c0afacb1a#body_text
is schema:about of	named entity 'DROMEDARY CAMEL' named entity 'BASED' named entity 'MERS CORONAVIRUS' named entity 'CAUSED BY' named entity 'SLOWER' named entity 'LIMITED' named entity 'TESTS' named entity 'SUGGEST' named entity 'GENERATED' named entity 'POSITIVE FOR' named entity 'MERS-COV' named entity 'SPIKE' named entity 'ZOONOTIC DISEASE' named entity 'HUMAN' named entity 'ETHIOPIA' named entity 'S PROTEIN' named entity 'CONCENTRATIONS' named entity 'TYPE' named entity 'THESE' named entity 'CASES' named entity 'INDICATING' named entity 'COLLECTED' named entity 'NORTH AFRICA' named entity 'VIRAL REPLICATION' named entity 'SPECIFIC' named entity 'GENETIC TESTS' named entity 'SLOWLY' named entity 'MIDDLE EAST' named entity 'human' named entity 'genetic tests' named entity 'protein' named entity 'woreda' named entity 'cultured cells' named entity 'region' named entity 'MERS' named entity 'African' named entity 'Ethiopian' named entity 'zoonotic disease' named entity 'common' named entity 'Ethiopia' named entity 'DIFFERENT' named entity 'DROMEDARIES' named entity 'VIRAL' named entity 'YOUNG' named entity 'CAUSE' named entity 'CONFIRMED BY' named entity 'RESERVOIR' named entity 'MIDDLE EAST RESPIRATORY SYNDROME ' named entity 'PROTEINS' named entity 'EXPERIMENTS' named entity 'SAUDI ARABIA' named entity 'RECOMBINANTS' named entity 'YEARS' named entity 'RESULTS' named entity 'PROTEIN SEQUENCES' named entity 'CAMEL' named entity '28S' named entity 'EASE' named entity 'OLD' named entity 'PERFORMED' named entity 'DUE TO' named entity 'MOUSE' named entity 'HAVE' named entity 'AMIBARA WOREDA' named entity 'CHIMERIC' named entity 'COATED' named entity 'VIROLOGICAL' named entity 'ACCOUNT' named entity 'TO TEST'

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