About: RNA interference inhibits hepatitis E virus mRNA accumulation and protein synthesis in vitro

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	RNA interference inhibits hepatitis E virus mRNA accumulation and protein synthesis in vitro
Creator	Yang, Zhibiao Zhang, Wen Cui, Li Hua, Xiuguo Yang, Shixing Yuan, Congli Huang, Fen Zhou, Junfang Zhu, Jianguo
Source	Elsevier; Medline; PMC
abstract	Hepatitis E virus (HEV) is a zoonotic pathogen to which several species, including human beings, pigs and rodents, are reported to be susceptible. To date, vaccines developed against HEV still need to be improved and a structural gene (ORF2), which encodes a capsid protein with high sequence conservation found across HEV genotypes, is a potential candidate. To exploit the possibility of using RNA interference (RNAi) as a strategy against HEV infection, four small interference RNA (siRNA) duplex targeting ORF2 gene were constructed. A challenge against HEV infection by RNAi was performed in A549 cells. Real-Time quantitative polymerase chain reaction (Real-Time qPCR) and Western blot assay demonstrated that four HEV specific siRNAs (si-ORF2-1, si-ORF2-2, si-ORF2-3 and si-ORF2-4) were capable of protecting cells against HEV infection with very high specificity and efficiency. The results suggest that RNAi is a potent anti-HEV infection prophylaxis strategy.
has issue date	2010-05-19(xsd:dateTime)
bibo:doi	10.1016/j.vetmic.2009.10.023
bibo:pmid	19963327
has license	no-cc
sha1sum (hex)	ba63294c4ff5f5a55e21e564d7af96c7ec6ed9a7
schema:url	https://doi.org/10.1016/j.vetmic.2009.10.023
resource representing a document's title	RNA interference inhibits hepatitis E virus mRNA accumulation and protein synthesis in vitro
has PubMed Central identifier	PMC7117326
has PubMed identifier	19963327
schema:publication	Vet Microbiol
resource representing a document's body	covid:ba63294c4ff5f5a55e21e564d7af96c7ec6ed9a7#body_text
is schema:about of	named entity 'ACCUMULATION' named entity 'MRNA' named entity 'HEPATITIS E VIRUS' named entity 'RNA INTERFERENCE' named entity 'PROTEIN SYNTHESIS' named entity 'IN VITRO' named entity 'hepatitis E virus' named entity 'protein synthesis' named entity 'A549' named entity 'EGFP' named entity 'RNA' named entity 'host cells' named entity 'genotype' named entity 'siRNAs' named entity 'expression vectors' named entity 'ORF2' named entity 'mRNA' named entity 'RNA interference' named entity 'primer' named entity 'RNA' named entity 'zoonotic' named entity 'HEV' named entity 'DMEM' named entity 'siRNAs' named entity 'ORF2' named entity 'transfected' named entity 'sequence conservation' named entity 'siRNA' named entity 'Qiagen' named entity 'HEV' named entity 'antiviral therapy' named entity 'RT-PCR' named entity 'gene' named entity 'A549' named entity 'HEV' named entity 'transcription' named entity 'siRNAs' named entity 'epidemiological' named entity 'gene' named entity 'serum' named entity 'siRNAs' named entity 'virus' named entity 'HEV' named entity 'human lung' named entity 'toxicity' named entity 'Lipofectamine' named entity 'primer' named entity 'loading control' named entity 'A549' named entity 'oncogenes' named entity 'reverse transcription' named entity 'siRNA' named entity 'HEV' named entity 'HEV' named entity 'Western blot' named entity 'A549' named entity 'primer' named entity 'vector' named entity 'siRNAs' named entity 'HEV' named entity 'ORF2' named entity 'siRNAs' named entity '1:500' named entity 'BamHI' named entity 'virus' named entity 'HEV' named entity '10 nM' named entity 'siRNAs' named entity 'transfected' named entity 'siRNAs' named entity 'HEV' named entity 'pathogen' named entity 'ORF2'

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