About: Predominance of G3B and G14 equine group A rotaviruses of a single VP4 serotype in Japan

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About: Predominance of G3B and G14 equine group A rotaviruses of a single VP4 serotype in Japan Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Predominance of G3B and G14 equine group A rotaviruses of a single VP4 serotype in Japan
Creator	Tsunemitsu, H Kawashima, K Imai, K Takagi, M Nishimori, T Higuchi, T Horino, R Imagawa, H Shouji, T Togo, M
Source	Medline; PMC
abstract	A total of 65 equine group A rotaviruses (GAR) isolated from diarrheal foals at 48 farms in Hokkaido, Japan, between 1996 (29 isolates) and 1997 (36 isolates) were characterized for their VP7 and VP4 serotypes by PCR, nucleotide sequencing, and virus neutralization (VN) tests. By PCR VP7 typing, all isolates were classified as G3 or G14, and the predominant serotype in each year was G3 (86%) in 1996 and G14 (53%) in 1997. VN tests with these 20 isolates randomly selected confirmed the specificity of PCR on the bases of complete agreement of the results in these methods (9 G3 and 11 G14), and revealed that all 9 G3 isolates were subtype G3B. There were five differing amino acid residues in three VP7 antigenic regions between subtypes G3A and G3B. Antiserum to a baculovirus recombinant that expressed P[12] VP4 neutralized all isolates and P[12] reference strains. These results suggest that genotype P[12] GAR belong to a single VP4 serotype, and that one VP4 and two VP7 serotypes (G3B and G14) of GAR were predominant in the equine population in Japan.
has issue date	2001-01-01(xsd:dateTime)
bibo:doi	10.1007/s007050170044
bibo:pmid	11722016
has license	no-cc
sha1sum (hex)	bb4ad16a3948603d33b96dc5fde9aec0c1a83adb
schema:url	https://doi.org/10.1007/s007050170044
resource representing a document's title	Predominance of G3B and G14 equine group A rotaviruses of a single VP4 serotype in Japan
has PubMed Central identifier	PMC7087255
has PubMed identifier	11722016
schema:publication	Arch Virol
resource representing a document's body	covid:bb4ad16a3948603d33b96dc5fde9aec0c1a83adb#body_text
is schema:about of	named entity 'serotypes' named entity 'isolates' named entity 'G14' named entity 'isolates' named entity 'single' named entity 'serotype' named entity 'ISOLATES' named entity '1997' named entity 'tests' named entity 'GAR' named entity 'PCR' named entity 'Japan' named entity 'antigenic' named entity 'GAR' named entity '1996' named entity 'isolates' named entity 'VP4' named entity 'antigenic' named entity 'Japan' named entity 'VP7' named entity 'recombinant' named entity 'serotypes' named entity 'serotype' named entity 'PCR' named entity 'Japan' named entity 'Japan' named entity 'NotI' named entity 'viruses' named entity 'serotype' named entity 'VP7' named entity 'Thoroughbred' named entity 'VP7' named entity 'restriction fragment length polymorphism' named entity 'BamHI' named entity 'antibody' named entity 'serotypes' named entity 'Australia' named entity 'epitopes' named entity 'RT-PCR' named entity 'Australia' named entity 'bovine' named entity 'PCR' named entity 'viruses' named entity 'VP7' named entity 'serotypes' named entity 'porcine' named entity 'vector' named entity 'Thoroughbred' named entity 'cloned' named entity 'Tris-HCl' named entity 'PCR' named entity 'PCR' named entity 'recombinant' named entity 'Freund's adjuvant' named entity 'PCR' named entity 'VP7' named entity 'VP7' named entity 'VP7' named entity 'reassortants' named entity 'fluorescent' named entity 'dimethyl sulfoxide' named entity 'genotypes' named entity 'PCR' named entity 'serotypes' named entity 'serotypes' named entity 'feces' named entity 'hybridization' named entity 'serotype' named entity 'virions' named entity 'latex agglutination test' named entity 'baculovirus' named entity 'amino acids'

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