About: The expression of membrane protein augments the specific responses induced by SARS-CoV nucleocapsid DNA immunization

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About: The expression of membrane protein augments the specific responses induced by SARS-CoV nucleocapsid DNA immunization Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The expression of membrane protein augments the specific responses induced by SARS-CoV nucleocapsid DNA immunization
Creator	Qin, Chuan Yang, Ying Liang, Guo-Dong Li, Yin-Chuan Peng, Jing-Pian Shi, Shu-Qun Sun, Quan-Hong Wang, Jin-Ling Xu, Li
Source	Elsevier; Medline; PMC
abstract	Abstract Nucleocapsid protein plays a critical role in SARS-CoV pathogenesis, and high-level anti-nucleocapsid antibodies are detected in the patients infected by severe acute respiratory syndrome-associated coronavirus (SARS-CoV). Several studies have shown that there exists an interaction between nucleocapsid (N) and membrane (M) protein. In this paper, we investigate whether the expression of membrane protein can affect the immune responses induced by nucleocapsid DNA immunization. Two recombinant plasmids containing M and N coding sequence were constructed. Moreover, in order to get the antigen for ELISA and in vitro stimulation assay, N protein were expressed and purified from E. coli bacteria. Injection of 20μg of the mixture of pVAX1-M and pVAX1-N into the Balb/c mice could elicit the humoral and cellular responses. The ELISA analysis using the N antigen or inactivated SARS-CoV particles as capture antigen showed that co-injection of SARS-M could enhance N-induced antibody production, especially IgG2a subclass. After lymphocytes were stimulated with 10μg/ml purified N antigen, The CD4+ and CD8+ T cells of N and M plus N group were increased compared with those of control groups, and the M protein could augment the activation of lymphocytes induced by N DNA vaccine. Cytokine ELISA analysis revealed that co-injection of M could enhance the levels of IFN-γ, IL-2 release induced by N antigen. Further experiments in field mouse also support the claim that membrane protein can augment the N-specific immune responses. Virus challenge test was conducted in BSL3 bio safety laboratory with Brandt's vole SARS-CoV model, and the results indicated that co-immunization of M and N antigens could reduce the mortality and pathological changes in lung from the virus infection.
has issue date	2006-04-30(xsd:dateTime)
bibo:doi	10.1016/j.molimm.2005.11.005
bibo:pmid	16423399
has license	els-covid
sha1sum (hex)	bb4df91230a99d430331add3f689d2af441b83a1
schema:url	https://doi.org/10.1016/j.molimm.2005.11.005
resource representing a document's title	The expression of membrane protein augments the specific responses induced by SARS-CoV nucleocapsid DNA immunization
has PubMed Central identifier	PMC7112551
has PubMed identifier	16423399
schema:publication	Molecular Immunology
resource representing a document's body	covid:bb4df91230a99d430331add3f689d2af441b83a1#body_text
is schema:about of	named entity 'Two' named entity 'acute' named entity 'antigen' named entity 'release' named entity 'laboratory' named entity 'lymphocytes' named entity 'IL-2' named entity 'control groups' named entity 'experiments' named entity 'stimulation' named entity 'cellular' named entity 'bacteria' named entity 'nucleocapsid' named entity 'EXPRESSION' named entity 'DNA IMMUNIZATION' named entity 'INCREASED' named entity 'ORDER' named entity 'INFECTED' named entity 'MEMBRANE PROTEIN' named entity 'ANTIBODY PRODUCTION' named entity 'ANTIGEN' named entity 'LABORATORY' named entity 'REVEALED' named entity 'CYTOKINE ' named entity 'ANTIBODIES' named entity 'PLASMIDS' named entity 'COMPARED' named entity 'ANTIGENS' named entity 'INVESTIGATE' named entity 'SUBCLASS' named entity 'SAFETY' named entity 'PATHOLOGICAL' named entity 'CONSTRUCTED' named entity 'MODEL' named entity 'DETECTED' named entity 'FIELD MOUSE' named entity 'CONTROL GROUPS' named entity 'CODING SEQUENCE' covid:arg/bb4df91230a99d430331add3f689d2af441b83a1 named entity 'INDICATED' named entity 'MEMBRANE' named entity 'LEVELS' named entity 'EXPRESSION' named entity 'PROTEIN' named entity 'MIXTURE' named entity 'VOLE' named entity 'NUCLEOCAPSID PROTEIN' named entity 'PAPER' named entity 'VIRUS INFECTION' named entity 'PURIFIED' named entity 'CONTAINING' named entity 'AFFECT' named entity 'MEMBRANE PROTEIN' named entity 'CD4' named entity 'RESPONSES' named entity 'NUCLEOCAPSID' named entity 'SARS-COV' named entity 'RESPONSES' named entity 'IMMUNIZATION' named entity 'RECOMBINANT' named entity 'PROTEIN ' named entity 'PROTEIN CAN' named entity 'LYMPHOCYTES' named entity 'ASSOCIATED' named entity 'SARS' named entity 'CORONAVIRUS' named entity 'INJECTION' named entity 'IGG2A' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'M PROTEIN' named entity 'ENHANCE' named entity 'DNA VACCINE' named entity 'VIRUS'

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