About: A novel reporter system for neutralizing and enhancing antibody assay against dengue virus

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About: A novel reporter system for neutralizing and enhancing antibody assay against dengue virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A novel reporter system for neutralizing and enhancing antibody assay against dengue virus
Creator	Zhao, Hui Deng, Yong-Qiang Qin, Cheng-Feng Zhang, Bo Shi, Pei-Yong Jiang, Tao Li, Xiao-Feng Jiang, Zhen-You Qin, E-De Song, Ke-Yu Zhang, Fu-Chun Zhu, Shun-Ya
Source	Medline; PMC
abstract	BACKGROUND: Dengue virus (DENV) still poses a global public health threat, and no vaccine or antiviral therapy is currently available. Antibody plays distinct roles in controlling DENV infections. Neutralizing antibody is protective against DENV infection, whereas sub-neutralizing concentration of antibody can increase DENV infection, termed antibody-dependent enhancement (ADE). Plaque-based assay represents the most widely accepted method measuring neutralizing or enhancing antibodies. RESULTS: In this study, a novel reporter virus-based system was developed for measuring neutralization and ADE activity. A stable Renilla luciferase reporter DENV (Luc-DENV) that can produce robust luciferase signals in BHK-21 and K562 cells were used to establish the assay and validated against traditional plaque-based assay. Luciferase value analysis using various known DENV-specific monoclonal antibodies showed good repeatability and a well linear correlation with conventional plaque-based assays. The newly developed assay was finally validated with clinical samples from infected animals and individuals. CONCLUSIONS: This reporter virus-based assay for neutralizing and enhancing antibody evaluation is rapid, lower cost, and high throughput, and will be helpful for laboratory detection and epidemiological investigation for DENV antibodies.
has issue date	2014-02-18(xsd:dateTime)
bibo:doi	10.1186/1471-2180-14-44
bibo:pmid	24548533
has license	cc-by
sha1sum (hex)	bb6a9f522a87a780723faca7cde002ece6dbfb48
schema:url	https://doi.org/10.1186/1471-2180-14-44
resource representing a document's title	A novel reporter system for neutralizing and enhancing antibody assay against dengue virus
has PubMed Central identifier	PMC3930823
has PubMed identifier	24548533
schema:publication	BMC Microbiol
resource representing a document's body	covid:bb6a9f522a87a780723faca7cde002ece6dbfb48#body_text
is schema:about of	named entity 'accepted' named entity 'ADE' named entity 'antiviral therapy' named entity 'ENHANCING' named entity 'NOVEL' named entity 'BACKGROUND' named entity 'NEUTRALIZING ANTIBODY' named entity 'PLAQUE' named entity 'ANTIBODY' named entity 'METHOD' named entity 'ACCEPTED' named entity 'ANTIVIRAL THERAPY' named entity 'PUBLIC HEALTH' named entity 'CURRENTLY' named entity 'VACCINE' named entity 'SYSTEM' named entity 'CONTROLLING' named entity 'AVAILABLE' named entity 'INCREASE' named entity 'ASSAY' named entity 'GLOBAL' named entity 'BASED' named entity 'DENGUE VIRUS' named entity 'ANTIBODY' named entity 'ASSAY' named entity 'REPORTER' named entity 'ENHANCING ANTIBODIES' named entity 'CONCENTRATION OF' named entity 'SUB-' named entity 'INFECTION' named entity 'MEASURING' named entity 'DENGUE VIRUS' named entity 'ROLES' named entity 'PLAYS' named entity 'INFECTIONS' named entity 'THREAT' named entity 'ANTIBODY-DEPENDENT ENHANCEMENT' named entity 'DISTINCT' covid:arg/bb6a9f522a87a780723faca7cde002ece6dbfb48 named entity 'DENV' named entity 'enhancing' named entity 'controlling' named entity 'antibody' named entity 'antibody' named entity 'ADE' named entity 'infection' named entity 'global public health' named entity 'assay' named entity 'antibody' named entity 'mAbs' named entity 'SPSS' named entity 'DENV' named entity 'ADE' named entity 'Invitrogen' named entity 'DENV' named entity 'penicillin' named entity 'antibody' named entity 'gene' named entity 'FcR' named entity 'dengue virus' named entity 'African green monkey' named entity 'BHK-21 cells' named entity 'antibodies' named entity '0.45' named entity 'cell lysates' named entity 'plaque assay' named entity 'virus' named entity 'ADE' named entity 'antiviral' named entity 'DENV'

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