About: Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5′ poly(A) leader

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About: Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5′ poly(A) leader Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5′ poly(A) leader
Creator	Schwer, Beate Stunnenberg, Hendrik Vista, Paolo Vos, Jan
Source	Elsevier; Medline; PMC
abstract	Abstract We have demonstrated by primer elongation and cap analysis that mature vaccinia virus late transcripts are discontinuously synthesized. We have shown that RNA transcripts from a translocated 11K and from the authentic 11K and 4b late promoters are extended by approximately 35 nucleotides beyond the “start site” determined by S1 mapping using vaccinia genomic DNA as a probe. Sequencing of the RNA and of the first strand cDNA reveal that a homopolymeric poly(A) sequence is linked to the 5′ terminus of the RNA transcripts. S1 mapping of RNA transcripts with a DNA probe containing an A-stretch, replacing promoter sequences upstream of position −1, confirms the existence of a poly(A) leader of approximately 35 A-residues.
has issue date	1987-07-17(xsd:dateTime)
bibo:doi	10.1016/0092-8674(87)90212-1
bibo:pmid	3594569
has license	els-covid
sha1sum (hex)	bb790e8366da63c4f5e2d64fa7bbd5673b93063c
schema:url	https://doi.org/10.1016/0092-8674%2887%2990212-1
resource representing a document's title	Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5′ poly(A) leader
has PubMed Central identifier	PMC7133236
has PubMed identifier	3594569
schema:publication	Cell
resource representing a document's body	covid:bb790e8366da63c4f5e2d64fa7bbd5673b93063c#body_text
is schema:about of	named entity 'Leader' named entity 'RESULTS' named entity 'POLY' named entity 'DISCONTINUOUS' named entity 'TRANSCRIPTION' named entity '28A' named entity 'LATE' named entity 'VACCINIA VIRUS' named entity 'MESSENGERS' named entity 'RNA PROCESSING' named entity 'negative control' named entity 'vaccinia' named entity 'transcription initiation' named entity '5' end' named entity 'RNA' named entity 'homopolymeric' named entity 'reverse transcriptase' named entity 'RNA leader' named entity 'Okayama' named entity 'cDNAs' named entity 'm7G' named entity 'cloned' named entity '5' end' named entity 'mRNA' named entity 'primer extension' named entity 'vaccinia' named entity 'RNAase' named entity '5' end' named entity 'primer extension' named entity '5' end' named entity 'RNAase' named entity 'molecule' named entity 'enzyme' named entity 'DNA probe' named entity 'leader RNA' named entity 'cell culture' named entity 'cDNA' named entity 'virus' named entity 'protein' named entity 'cDNA' named entity 'regulation of gene expression' named entity 'RNAase' named entity 'Sweden' named entity 'motif' named entity 'RNA' named entity 'start codon' named entity 'homologous recombination' named entity 'dideoxynucleotides' named entity 'RNA' named entity 'nuclease' named entity 'influenza virus' named entity 'RNA transcripts' named entity 'polyacrylamide gel' named entity 'precipitate' named entity 'polyclonal' named entity 'cDNA' named entity 'polypeptide' named entity 'nucleotides' named entity 'nibbling' named entity 'wild-type' named entity 'genomic sequence' named entity 'gene' named entity 'translocation' named entity 'vaccinia' named entity 'RNA' named entity 'functional analysis' named entity 'vaccinia virus' named entity 'hybrids' named entity 'digestion' named entity 'RNAase' named entity 'mRNA' named entity 'oligonucleotide' named entity 'nucleotides' named entity '3' ends' named entity 'vaccinia' named entity 'gene' named entity 'leader RNA' named entity 'nucleotides'

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