About: Phosphatidic Acid Produced by Phospholipase D Promotes RNA Replication of a Plant RNA Virus

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About: Phosphatidic Acid Produced by Phospholipase D Promotes RNA Replication of a Plant RNA Virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Phosphatidic Acid Produced by Phospholipase D Promotes RNA Replication of a Plant RNA Virus
Creator	Hyodo, Kiwamu Kaido, Masanori Manabe, Yuki Mise, Kazuyuki Okuno, Tetsuro Sugawara, Tatsuya Taniguchi, Hisaaki Taniguchi, Takako
Source	Medline; PMC
abstract	Eukaryotic positive-strand RNA [(+)RNA] viruses are intracellular obligate parasites replicate using the membrane-bound replicase complexes that contain multiple viral and host components. To replicate, (+)RNA viruses exploit host resources and modify host metabolism and membrane organization. Phospholipase D (PLD) is a phosphatidylcholine- and phosphatidylethanolamine-hydrolyzing enzyme that catalyzes the production of phosphatidic acid (PA), a lipid second messenger that modulates diverse intracellular signaling in various organisms. PA is normally present in small amounts (less than 1% of total phospholipids), but rapidly and transiently accumulates in lipid bilayers in response to different environmental cues such as biotic and abiotic stresses in plants. However, the precise functions of PLD and PA remain unknown. Here, we report the roles of PLD and PA in genomic RNA replication of a plant (+)RNA virus, Red clover necrotic mosaic virus (RCNMV). We found that RCNMV RNA replication complexes formed in Nicotiana benthamiana contained PLDα and PLDβ. Gene-silencing and pharmacological inhibition approaches showed that PLDs and PLDs-derived PA are required for viral RNA replication. Consistent with this, exogenous application of PA enhanced viral RNA replication in plant cells and plant-derived cell-free extracts. We also found that a viral auxiliary replication protein bound to PA in vitro, and that the amount of PA increased in RCNMV-infected plant leaves. Together, our findings suggest that RCNMV hijacks host PA-producing enzymes to replicate.
has issue date	2015-05-28(xsd:dateTime)
bibo:doi	10.1371/journal.ppat.1004909
bibo:pmid	26020241
has license	cc-by
sha1sum (hex)	bbe42ce008a9dd9e25c03c36113d763dba358cbb
schema:url	https://doi.org/10.1371/journal.ppat.1004909
resource representing a document's title	Phosphatidic Acid Produced by Phospholipase D Promotes RNA Replication of a Plant RNA Virus
has PubMed Central identifier	PMC4447390
has PubMed identifier	26020241
schema:publication	PLoS Pathog
resource representing a document's body	covid:bbe42ce008a9dd9e25c03c36113d763dba358cbb#body_text
is schema:about of	named entity 'RNA' named entity 'replicate' named entity 'modify' named entity 'multiple' named entity 'replication' named entity 'enzyme' named entity 'RNA' named entity 'RNA Virus' named entity 'RNA' named entity 'COMPONENTS' covid:arg/bbe42ce008a9dd9e25c03c36113d763dba358cbb named entity 'AMOUNTS' named entity 'OBLIGATE' named entity 'UNKNOWN' named entity 'GENOMIC' named entity 'VARIOUS' named entity 'PROTEIN BOUND' named entity 'DIVERSE' named entity 'INCREASED' named entity 'RESPONSE TO' named entity 'AUXILIARY' named entity 'SMALL' named entity 'VIRUSES' named entity 'INTRACELLULAR' named entity 'PRODUCTION' named entity 'RNA VIRUS' named entity 'GENE' named entity 'BOUND' named entity 'SILENCING' named entity 'ROLES' named entity 'VIRUS' named entity 'OUR' named entity 'NICOTIANA BENTHAMIANA' named entity 'PHOSPHOLIPASE D' named entity 'PHOSPHATIDIC ACID' named entity 'PLANT RNA' named entity 'POSITIVE' named entity 'HOST' named entity 'ENVIRONMENTAL' named entity 'PLANTS' named entity 'COMPLEXES' named entity 'ENHANCED' named entity 'INFECTED' named entity 'CONSISTENT WITH' named entity 'ORGANISMS' named entity 'CONTAIN' named entity 'REPLICATE' named entity 'REQUIRED' named entity 'EXPLOIT' named entity 'LIPID BILAYERS' named entity 'PARASITES' named entity 'FUNCTIONS' named entity 'REPLICATION PROTEIN' named entity 'USING' named entity 'APPLICATION' named entity 'FORMED' named entity 'SECOND MESSENGER' named entity 'REPLICASE' named entity 'DERIVED' named entity 'STRAND' named entity 'APPROACHES' named entity 'HERE' named entity 'PHOSPHATIDIC ACID' named entity 'PHOSPHOLIPASE D' named entity 'RNA REPLICATION' named entity 'FINDINGS' named entity 'STRESSES' named entity 'A LIPID' named entity 'PHARMACOLOGICAL' named entity 'PLANT-' named entity 'ENZYMES' named entity 'RAPIDLY' named entity 'MODIFY' named entity 'RESOURCES'

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