About: Broad-Spectrum Detection of H5 Subtype Influenza A Viruses with a New Fluorescent Immunochromatography System

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Broad-Spectrum Detection of H5 Subtype Influenza A Viruses with a New Fluorescent Immunochromatography System
Creator	Yamamoto, N Munakata, T Nomura, N Sakurai, A Takayama, K
Source	Medline; PMC
abstract	Immunochromatography (IC) is an antigen-detection assay that plays an important role in the rapid diagnosis of influenza virus because the protocol is short time and easy to use. Despite the usability of IC, the sensitivity is approximately 10(3) pfu per reaction. In addition, antigen-antibody interaction-based method cannot be used for the detection of influenza viruses with major antigenic change. In this study, we established the use of fluorescent immunochromatography (FLIC) to detect a broad spectrum of H5 subtype influenza A viruses. This method has improved sensitivity 10–100 fold higher than traditional IC because of the use of fluorescent conjugated beads. Our Type-E FLIC kit detected all of the H5 subtype influenza viruses that were examined, as well as recombinant hemagglutinin (HA) proteins (rHAs) belonging to the Eurasian H5 subtype viruses and the Type-N diagnosed North American H5 subtype influenza A viruses. Thus, this kit has the improved potential to detect H5 subtype influenza viruses of different clades with both Type-E and Type-N FLIC kits. Compared with PCR-based diagnosis, FLIC has a strong advantage in usability, because the sample preparation required for FLIC is only mix-and-drop without any additional steps such as RNA extraction. Our results can provide new strategies against the spread and transmission of HPAI H5N1 viruses in birds and mammals including humans.
has issue date	2013-11-06(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0076753
bibo:pmid	24223117
has license	cc-by
sha1sum (hex)	bc381e908d987cbecf5fb3df2640a7c878886405
schema:url	https://doi.org/10.1371/journal.pone.0076753
resource representing a document's title	Broad-Spectrum Detection of H5 Subtype Influenza A Viruses with a New Fluorescent Immunochromatography System
has PubMed Central identifier	PMC3819354
has PubMed identifier	24223117
schema:publication	PLoS One
resource representing a document's body	covid:bc381e908d987cbecf5fb3df2640a7c878886405#body_text
is schema:about of	named entity 'beads' named entity 'Despite' named entity 'SPECTRUM' covid:arg/bc381e908d987cbecf5fb3df2640a7c878886405 named entity 'INFLUENZA VIRUSES' named entity 'SENSITIVITY' named entity 'RAPID DIAGNOSIS' named entity 'REQUIRED' named entity 'SPREAD' named entity 'CONJUGATED' named entity 'MAJOR' named entity 'HPAI' named entity 'SHORT' named entity 'IMPROVED' named entity 'BIRDS' named entity 'KIT' named entity 'IMPORTANT' named entity 'METHOD' named entity 'SAMPLE PREPARATION' named entity 'INCLUDING' named entity 'ADDITIONAL' named entity 'TIME' named entity 'USE' named entity 'FOLD' named entity 'VIRUSES' named entity 'NEW' named entity 'DIAGNOSED' named entity 'DROP' named entity 'USED' named entity 'ADDITION' named entity 'MAMMALS' named entity 'ASSAY' named entity 'USE OF' named entity 'COMPARED' named entity 'DIAGNOSIS' named entity 'RECOMBINANT' named entity 'BROAD SPECTRUM' named entity 'H5N1' named entity 'PLAYS' named entity 'TRANSMISSION' named entity 'DIFFERENT' named entity 'POTENTIAL' named entity 'HEMAGGLUTININ' named entity 'HIGHER' named entity 'BELONGING' named entity 'RNA EXTRACTION' named entity 'STEPS' named entity 'TO DETECT' named entity 'PROTEINS' named entity 'IMMUNOCHROMATOGRAPHY' named entity 'APPROXIMATELY' named entity 'ANTIGEN' named entity 'TRADITIONAL' named entity 'VIRUSES' named entity 'DETECTED' named entity 'CHANGE' named entity 'SYSTEM' named entity 'OUR' named entity 'BROAD' named entity 'INFLUENZA A' named entity 'IMMUNOCHROMATOGRAPHY' named entity 'FLUORESCENT' named entity 'SUBTYPE' named entity 'DETECTION' named entity 'HUMANS' named entity '100' named entity 'SUBTYPE' named entity 'ADVANTAGE' named entity 'ESTABLISHED' named entity 'RESULTS' named entity 'REACTION' named entity 'EASY' named entity 'PFU' named entity 'INFLUENZA A' named entity 'STRONG' named entity 'STUDY' named entity 'PCR'

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