About: Porcine Hemagglutinating Encephalomyelitis Virus Activation of the Integrin α5β1-FAK-Cofilin Pathway Causes Cytoskeletal Rearrangement To Promote Its Invasion of N2a Cells

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Porcine Hemagglutinating Encephalomyelitis Virus Activation of the Integrin α5β1-FAK-Cofilin Pathway Causes Cytoskeletal Rearrangement To Promote Its Invasion of N2a Cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Porcine Hemagglutinating Encephalomyelitis Virus Activation of the Integrin α5β1-FAK-Cofilin Pathway Causes Cytoskeletal Rearrangement To Promote Its Invasion of N2a Cells
Creator	He, Hongbin Zhang, Jing Gao, Feng He, Wenqi Lan, Yungang Lu, Huijun Song, Deguang Zhao, Kui Li, Zi Lv, Xiaoling Guan, Jiyu Hu, Shiyu
Source	Medline; PMC
abstract	Porcine hemagglutinating encephalomyelitis virus (PHEV) is a highly neurotropic virus that causes diffuse neuronal infection with neurological damage and high mortality. Virus-induced cytoskeletal dynamics are thought to be closely related to this type of nerve damage. Currently, the regulation pattern of the actin cytoskeleton and its molecular mechanism remain unclear when PHEV enters the host cells. Here, we demonstrate that entry of PHEV into N2a cells induces a biphasic remodeling of the actin cytoskeleton and a dynamic change in cofilin activity. Viral entry is affected by the disruption of actin kinetics or alteration of cofilin activity. PHEV binds to integrin α5β1 and then initiates the integrin α5β1-FAK signaling pathway, leading to virus-induced early cofilin phosphorylation and F-actin polymerization. Additionally, Ras-related C3 botulinum toxin substrate 1 (Rac1), cell division cycle 42 (Cdc42), and downstream regulatory gene p21-activated protein kinases (PAKs) are recruited as downstream mediators of PHEV-induced dynamic changes of the cofilin activity pathway. In conclusion, we demonstrate that PHEV utilizes the integrin α5β1-FAK-Rac1/Cdc42-PAK-LIMK-cofilin pathway to cause an actin cytoskeletal rearrangement to promote its own invasion, providing theoretical support for the development of PHEV pathogenic mechanisms and new antiviral targets. IMPORTANCE PHEV, a member of the Coronaviridae family, is a typical neurotropic virus that primarily affects the nervous system of piglets to produce typical neurological symptoms. However, the mechanism of nerve damage caused by the virus has not been fully elucidated. Actin is an important component of the cytoskeleton of eukaryotic cells and serves as the first obstacle to the entry of pathogens into host cells. Additionally, the morphological structure and function of nerve cells depend on the dynamic regulation of the actin skeleton. Therefore, exploring the mechanism of neuronal injury induced by PHEV from the perspective of the actin cytoskeleton not only helps elucidate the pathogenesis of PHEV but also provides a theoretical basis for the search for new antiviral targets. This is the first report to define a mechanistic link between alterations in signaling from cytoskeleton pathways and the mechanism of PHEV invading nerve cells.
has issue date	2018-12-12(xsd:dateTime)
bibo:doi	10.1128/jvi.01736-18
bibo:pmid	30541856
has license	bronze-oa
sha1sum (hex)	bc5fa241fb3076e2cff1e121d6bd7566ae1c52eb
schema:url	https://doi.org/10.1128/jvi.01736-18
resource representing a document's title	Porcine Hemagglutinating Encephalomyelitis Virus Activation of the Integrin α5β1-FAK-Cofilin Pathway Causes Cytoskeletal Rearrangement To Promote Its Invasion of N2a Cells
has PubMed Central identifier	PMC6384086
has PubMed identifier	30541856
schema:publication	Journal of Virology
resource representing a document's body	covid:bc5fa241fb3076e2cff1e121d6bd7566ae1c52eb#body_text
is schema:about of	named entity 'damage' named entity 'cytoskeleton' named entity 'cytoskeleton' named entity 'remodeling' named entity 'integrin' named entity 'define' named entity 'piglets' named entity 'depend' named entity 'rearrangement' named entity 'alterations' named entity 'PHEV' named entity 'neuronal' named entity 'demonstrate' named entity 'link' named entity 'helps' named entity 'Encephalomyelitis' named entity 'Cdc42' named entity 'Porcine' named entity 'mortality' named entity 'mechanistic' named entity 'high' named entity 'Cdc42' named entity 'Actin' named entity 'pathogens' named entity 'actin' named entity 'entry' named entity 'nerve damage' named entity 'induces' named entity 'activity' named entity 'However' named entity 'type' named entity 'antiviral' named entity 'family' named entity 'actin' named entity 'mediators' named entity 'protein' named entity 'dynamic' named entity 'pathogenesis' named entity 'highly' named entity 'Invasion' named entity 'encephalomyelitis' named entity 'cofilin' named entity 'virus' named entity 'actin' named entity 'polymerization' named entity 'cytoskeletal' named entity 'nerve cells' named entity 'botulinum toxin' named entity 'PHEV' named entity 'virus' named entity 'PHEV' named entity 'virus' named entity 'Coronaviridae' named entity 'nerve damage' named entity 'cofilin' named entity 'morphological' named entity 'Porcine' named entity 'eukaryotic cells' named entity 'actin' named entity 'regulatory gene' named entity 'cytoskeleton' named entity 'infection' named entity 'Encephalomyelitis' named entity 'Hemagglutinating' named entity 'Cofilin' named entity 'Integrin' named entity 'primary antibodies' named entity 'cofilin' named entity 'PHEV' named entity 'cell surface'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software