About: A comparative assessment of four serological methods used in the detection and measurement of anti-parasite antibodies in the serum of the amphibian, bufo viridis

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About: A comparative assessment of four serological methods used in the detection and measurement of anti-parasite antibodies in the serum of the amphibian, bufo viridis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A comparative assessment of four serological methods used in the detection and measurement of anti-parasite antibodies in the serum of the amphibian, bufo viridis
Creator	Al-Yaman, Fadwa Ingram, George
Source	Elsevier; PMC
abstract	Abstract Antibodies against Crithidia fasciculata choanomastigotes were detected in green toad (Bufo viridis) sera by direct agglutination, indirect haemagglutination (IHA), complement-fixation test (CFT) and enzyme-linked immunosorbent assay (ELISA), Correlation coefficients (r) were calculated for comparisons between each of the techniques and regression formulae derived in order to convert antibody levels as determined by one immunological method to that of another. The highest mean titre obtained by ELISA was approximately 1.5–3.5 times greater than those obtained by the other techniques whilst CFT gave the lowest values. IHA and ELISA titres were affected by different preparations of the crithidial antigen extracts. Highly significant r values were determined for control sera when IHA was compared to ELISA (r > 0.79), and to both CFT and ELISA with immune animals (r > 0.96). ELISA would seem most applicable for screening other lower vertebrates for anti-parasite antibodies especially in areas of human disease prevalence.
has issue date	1988-04-30(xsd:dateTime)
bibo:doi	10.1016/0020-7519(88)90147-6
has license	els-covid
sha1sum (hex)	bcea326da7ffbd034aa531671d14f0b907e561cc
schema:url	https://doi.org/10.1016/0020-7519%2888%2990147-6
resource representing a document's title	A comparative assessment of four serological methods used in the detection and measurement of anti-parasite antibodies in the serum of the amphibian, bufo viridis
has PubMed Central identifier	PMC7130362
schema:publication	International Journal for Parasitology
resource representing a document's body	covid:bcea326da7ffbd034aa531671d14f0b907e561cc#body_text
is schema:about of	named entity 'IHA' named entity 'AMPHIBIAN' named entity 'DIRECT AGGLUTINATION' named entity 'ANTIBODIES' named entity 'DETECTED' named entity 'JOURNAL ' named entity 'SEROLOGICAL' named entity 'HAEMAGGLUTINATION' named entity 'CORRELATION' named entity 'USED' named entity 'ASSESSMENT' named entity 'TECHNIQUES' named entity 'PARASITOLOGY' named entity 'METHODS' named entity 'PARASITE' named entity 'ANTIBODIES' named entity 'USED' named entity 'SERUM' named entity 'BUFO' named entity 'MEASUREMENT' named entity 'MEASUREMENT' named entity 'SERUM' named entity 'REGRESSION' named entity 'METHODS' named entity 'SEROLOGICAL' named entity 'DETECTION' named entity 'TEST' named entity 'INTERNATIONAL' named entity 'ASSESSMENT' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' named entity 'COEFFICIENTS' named entity 'INDIRECT' named entity 'BUFO VIRIDIS' named entity 'COMPLEMENT-FIXATION' named entity 'DETECTION' named entity 'GREEN TOAD' named entity 'PARASITE' named entity 'SERA' named entity 'CALCULATED' named entity 'AMPHIBIAN' covid:arg/bcea326da7ffbd034aa531671d14f0b907e561cc named entity 'agglutination' named entity 'Parasitology' named entity 'Bufo viridis' named entity 'amphibian' named entity 'enzyme-linked immunosorbent assay' named entity 'CFT' named entity 'complement-fixation test' named entity 'agglutination' named entity 'haemagglutination' named entity 'AMPHIBIAN' named entity 'SEROLOGICAL' named entity 'SERUM' named entity 'BUFO' named entity 'DETECTION AND MEASUREMENT' named entity 'serum' named entity 'urease' named entity 'PBS' named entity 'experimental models' named entity 'immune sera' named entity 'parasite' named entity 'agglutination' named entity 'T. brucei' named entity 'body fluids' named entity 'infection' named entity 'agglutination' named entity 'immune sera' named entity 'antibody' named entity 'aetiological' named entity 'health status' named entity 'zoonoses' named entity 'parasite' named entity 'centrifuged' named entity 'living cells' named entity 'parasite' named entity 'similar manner' named entity 'antigen' named entity 'British Council' named entity '7.53' named entity 'antibodies' named entity 'immune sera' named entity 'protein'

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