About: Abstract Proteins fused to the elastin-like polypeptide (ELP) tag can be selectively separated from crude cell extract without chromatography. To avoid the interference of the ELP tag on properties of the target protein, it is necessary to remove the ELP tag from target protein by protease digestion. Therefore, an additional chromatographic purification step is required to remove the proteases, and this is time- and labor-consuming. Here we demonstrate the utility of the ELP-tagged proteases for cleavage of ELP fusion proteins, allowing one-step removal of the cleaved ELP tag and ELP-tagged proteases without chromatography.   Goto Sponge  NotDistinct  Permalink

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  • Abstract Proteins fused to the elastin-like polypeptide (ELP) tag can be selectively separated from crude cell extract without chromatography. To avoid the interference of the ELP tag on properties of the target protein, it is necessary to remove the ELP tag from target protein by protease digestion. Therefore, an additional chromatographic purification step is required to remove the proteases, and this is time- and labor-consuming. Here we demonstrate the utility of the ELP-tagged proteases for cleavage of ELP fusion proteins, allowing one-step removal of the cleaved ELP tag and ELP-tagged proteases without chromatography.
Subject
  • Biomaterials
  • Atlantic Records artists
  • English progressive rock groups
  • Island Records artists
  • Musical groups disestablished in 1979
  • Musical groups disestablished in 1998
  • Musical groups established in 1970
  • Musical groups reestablished in 1991
  • Musical groups reestablished in 2010
  • Symphonic rock groups
  • English art rock groups
  • British musical trios
  • Rock music supergroups
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