About: Development and evaluation of an antigen-capture ELISA for detection of the UL24 antigen of the duck enteritis virus, based on a polyclonal antibody against the UL24 expression protein

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About: Development and evaluation of an antigen-capture ELISA for detection of the UL24 antigen of the duck enteritis virus, based on a polyclonal antibody against the UL24 expression protein Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development and evaluation of an antigen-capture ELISA for detection of the UL24 antigen of the duck enteritis virus, based on a polyclonal antibody against the UL24 expression protein
Creator	Liu, Fei Cheng, Anchun Wang, Mingshu Guo, Yufei Jia, Renyong Chen, Xiaoyue Zhu, Dekang Luo, Qihui Qi, Xuefeng Ge, Han
Source	Elsevier; Medline; PMC
abstract	Abstract An antigen-capture enzyme-linked immunosorbent assay (AC-ELISA) method was developed for the efficient detection of the UL24 antigen of the duck enteritis virus (DEV) using polyclonal antibodies. Ducks and rabbits were immunized, respectively, with expressed UL24 recombinant protein. The IgG antibodies against UL24 from ducks and rabbits were purified and used as the capture antibodies. The specificity of the optimized AC-ELISA was evaluated by use of DEV, duck hepatitis virus (DHV), duck hepatitis B virus (DHBV), gosling plague virus (GPV), Riemerella anatipestifer (R.A.), Escherichia coli (E. coli), Pasteurella multocida (P.M.) and Salmonella Enteritidis (S.E.). Only DEV specimens yielded a specific and strong signal. The limit of the sensitivity of this method for the detection of DEV was 46ng/100μl. Compared with PCR and virus isolation, the rate of agreement for the detection of experimentally infected sera was 100%. A comparative test used on clinical specimens between the neutralization test and the AC-ELISA showed that the proportions of true positives and true negatives by the AC-ELISA were 0.90 and 0.67 respectively. These results indicated that the AC-ELISA approach is rapid, sensitive, and reliable for specific detection of DEV antigen.
has issue date	2009-10-31(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2009.05.011
bibo:pmid	19467266
has license	els-covid
sha1sum (hex)	bd5157e1e77a1f5e200a1c249e43f995ae44a715
schema:url	https://doi.org/10.1016/j.jviromet.2009.05.011
resource representing a document's title	Development and evaluation of an antigen-capture ELISA for detection of the UL24 antigen of the duck enteritis virus, based on a polyclonal antibody against the UL24 expression protein
has PubMed Central identifier	PMC7112936
has PubMed identifier	19467266
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:bd5157e1e77a1f5e200a1c249e43f995ae44a715#body_text
is schema:about of	named entity 'UL24' named entity 'specific' named entity 'ducks' named entity 'purified' named entity 'enzyme-linked immunosorbent assay' named entity 'antigen' named entity 'proportions' named entity 'test' named entity 'detection' named entity 'polyclonal antibody' named entity 'RIEMERELLA ANATIPESTIFER' named entity '28S' named entity 'SALMONELLA ENTERITIDIS' named entity 'DUCKS' named entity 'EVALUATED' named entity 'USED' named entity 'CLINICAL' named entity 'COMPARED' named entity 'RECOMBINANT PROTEIN' named entity 'ANTIGEN' named entity 'DEV' named entity 'VIRUS ISOLATION' named entity 'PURIFIED' named entity 'DUCK ENTERITIS VIRUS' named entity 'ELISA' named entity 'DUCK ENTERITIS VIRUS' named entity 'NEGATIVES' named entity 'CAPTURE' named entity 'LIMIT' named entity 'IGG' named entity 'POSITIVES' named entity 'SPECIFIC' named entity 'AGREEMENT' named entity 'VIRUS' named entity 'DUCK HEPATITIS VIRUS ' named entity '0.67' named entity 'RABBITS' named entity 'TRUE' named entity 'SPECIFICITY' named entity 'APPROACH' named entity 'RESULTS' named entity 'USING' named entity 'SERA' named entity 'INDICATED' named entity 'NEUTRALIZATION TEST' named entity 'ELISA' named entity 'PCR' named entity 'THESE' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' named entity 'RATE' named entity 'PASTEURELLA MULTOCIDA ' named entity 'PLAGUE' named entity 'ANTIBODIES' named entity 'IMMUNIZED' named entity 'EFFICIENT' named entity 'COMPARATIVE TEST' named entity '100%' named entity 'EXPRESSED' named entity 'DEVELOPMENT' named entity 'EVALUATION' named entity 'ANTIGEN' named entity 'BASED' named entity 'EXPRESSION PROTEIN' named entity 'RELIABLE' named entity 'DETECTION' named entity 'SPECIMENS' named entity 'UL24' named entity 'USE OF' named entity 'DUCK HEPATITIS B VIRUS' named entity 'RAPID' named entity 'POLYCLONAL ANTIBODIES' named entity 'STRONG'

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