About: Rapid colorimetric detection of COVID-19 coronavirus using a reverse tran-scriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic plat-form: iLACO

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Rapid colorimetric detection of COVID-19 coronavirus using a reverse tran-scriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic plat-form: iLACO Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Rapid colorimetric detection of COVID-19 coronavirus using a reverse tran-scriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic plat-form: iLACO
Creator	Li, Xin Liu, Na Yu, Lin Chen, Wei-Hua Dong, Xue Han, Heng Hao, Xiaowen Li, Jiyao Li, Xuelong Liu, Jianmin Liu, Xiyang Pelechano, Vicent Wu, Shanshan Ye, Shenglong Yin, Xiushan Zhang, Wanzhong
Source	MedRxiv
abstract	The recent outbreak of a novel coronavirus SARS-CoV-2 (also known as 2019-nCoV) threatens global health, given serious cause for concern. SARS-CoV-2 is a human-to-human pathogen that caused fever, severe respiratory disease and pneumonia (known as COVID-19). By press time, more than 70,000 infected people had been confirmed worldwide. SARS-CoV-2 is very similar to the severe acute respiratory syndrome (SARS) coronavirus broke out 17 years ago. However, it has increased transmissibility as compared with the SARS-CoV, e.g. very often infected individuals without any symptoms could still transfer the virus to others. It is thus urgent to develop a rapid, accurate and onsite diagnosis methods in order to effectively identify these early infects, treat them on time and control the disease spreading. Here we developed an isothermal LAMP based method-iLACO (isothermal LAMP based method for COVID-19) to amplify a fragment of the ORF1ab gene using 6 primers. We assured the species-specificity of iLACO by comparing the sequences of 11 related viruses by BLAST (including 7 similar coronaviruses, 2 influenza viruses and 2 normal coronaviruses). The sensitivity is comparable to Taqman based qPCR detection method, detecting synthesized RNA equivalent to 10 copies of 2019-nCoV virus. Reaction time varied from 15-40 minutes, depending on the loading of virus in the collected samples. The accuracy, simplicity and versatility of the new developed method suggests that iLACO assays can be conveniently applied with for 2019-nCoV threat control, even in those cases where specialized molecular biology equipment is not available.
has issue date	2020-02-24(xsd:dateTime)
bibo:doi	10.1101/2020.02.20.20025874
has license	medrxiv
sha1sum (hex)	bd8f57ea4aadeda075d557f566a1909d68e658c4
schema:url	https://doi.org/10.1101/2020.02.20.20025874
resource representing a document's title	Rapid colorimetric detection of COVID-19 coronavirus using a reverse tran-scriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic plat-form: iLACO
resource representing a document's body	covid:bd8f57ea4aadeda075d557f566a1909d68e658c4#body_text
is schema:about of	named entity 'PLAT' named entity 'The' named entity '2019-nCoV' named entity 'SARS-CoV-2' named entity 'Taqman' named entity 'cases' named entity 'RNA' named entity 'develop' named entity '40 minutes' named entity 'based' named entity 'assays' named entity 'SIMILAR' named entity 'ACCURACY' named entity 'TRANSFER' named entity 'YEARS' named entity 'REVERSE' named entity 'BLAST' named entity 'COLLECTED' named entity 'INFLUENZA VIRUSES' named entity 'VERY OFTEN' named entity 'VIRUS' named entity 'RAPID' named entity 'MOLECULAR BIOLOGY' named entity 'THREAT' named entity 'EARLY' named entity 'CORONAVIRUS' named entity 'NORMAL' named entity 'CONCERN' covid:arg/bd8f57ea4aadeda075d557f566a1909d68e658c4 named entity 'GLOBAL HEALTH' named entity 'SEVERE' named entity '70%' named entity 'CASES' named entity 'SPECIALIZED' named entity 'OUTBREAK' named entity 'EQUIPMENT' named entity 'ANY SYMPTOMS' named entity 'SPECIFICITY' named entity 'COMPARED' named entity 'RECENT' named entity 'PRIMERS' named entity 'TRAN' named entity 'DIAGNOSTIC' named entity 'TIME' named entity 'BASED' named entity 'DIAGNOSIS' named entity 'EQUIVALENT' named entity 'LOADING' named entity 'HERE' named entity 'LAMP' named entity 'FORM' named entity 'COVID-19' named entity 'USING' named entity 'CORONAVIRUS' named entity 'SARS-COV' named entity 'AMPLIFY' named entity 'WHERE' named entity 'DETECTING' named entity 'GIVEN' named entity 'RESPIRATORY DISEASE' named entity 'CONFIRMED' named entity 'CAUSE' named entity 'PNEUMONIA' named entity 'FRAGMENT OF' named entity 'VIRUSES' named entity 'DETECTION METHOD' named entity 'GENE' named entity 'SARS-COV-2' named entity 'QPCR' named entity 'URGENT'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software