About: Inhibition of porcine circovirus type 2 replication in mice by RNA interference

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Inhibition of porcine circovirus type 2 replication in mice by RNA interference
Creator	Kwang, Jimmy Chen, Isabelle Chua, Huikheng Du, Qingyun Liu, Jue
Source	Elsevier; Medline; PMC
abstract	Abstract Porcine circovirus type 2 (PCV2) is the primary causative agent of an emerging swine disease, postweaning multisystemic wasting syndrome (PMWS) for which no antiviral treatment is available. To exploit the possibility of using RNA interference (RNAi) as a therapeutic approach against the disease, plasmid-borne short hairpin RNAs (shRNAs) were generated to target the PCV2 genome. Transfection of these shRNAs into cultured PK15 cells caused a significant reduction in viral RNA production that was accompanied by inhibiting viral DNA replication and protein synthesis in infected cells. The effect was further tested in vivo in a mouse model that has been developed for PCV2 infection. Mice injected with shRNA before PCV2 infection showed substantially decreased microscopic lesions in inguinal lymph nodes compared to controls. In situ hybridization and immunohistochemical analyses showed that shRNA caused a significant inhibition in the level of viral DNA and protein synthesis detected in the lymph nodes of the treated mice relative to the controls. Taken together, these results indicate that shRNAs are capable of inhibiting PCV2 infection in vitro as well as in vivo and thus may constitute an effective therapeutic strategy for PCV2 infection.
has issue date	2006-04-10(xsd:dateTime)
bibo:doi	10.1016/j.virol.2005.12.006
bibo:pmid	16427679
has license	els-covid
sha1sum (hex)	be477ac1161b4b6eeaccb183ed41573d1d09efac
schema:url	https://doi.org/10.1016/j.virol.2005.12.006
resource representing a document's title	Inhibition of porcine circovirus type 2 replication in mice by RNA interference
has PubMed Central identifier	PMC7126151
has PubMed identifier	16427679
schema:publication	Virology
resource representing a document's body	covid:be477ac1161b4b6eeaccb183ed41573d1d09efac#body_text
is schema:about of	named entity 'REPLICATION' named entity 'cultured' named entity 'microscopic' named entity 'shRNA' named entity 'viral' named entity 'viral' named entity 'effective' named entity 'vivo' named entity 'INHIBITION' named entity 'SUBSTANTIALLY DECREASED' named entity 'TREATED' named entity 'TAKEN' named entity 'LESIONS' named entity 'MOUSE MODEL' named entity 'PK15' named entity 'INFECTED' named entity 'CAUSED' named entity 'VIRAL RNA' named entity 'RELATIVE TO' named entity 'LYMPH NODES' named entity 'EXPLOIT' named entity 'COMPARED' named entity 'INHIBITING' named entity 'INGUINAL LYMPH NODES' named entity 'EFFECTIVE' named entity 'CULTURED' named entity 'CAPABLE' named entity 'SHRNA' named entity 'MICE' named entity 'IN SITU HYBRIDIZATION' named entity 'DETECTED' named entity 'PROTEIN SYNTHESIS' named entity 'IN VITRO' covid:arg/be477ac1161b4b6eeaccb183ed41573d1d09efac named entity 'MICE' named entity 'PRODUCTION' named entity 'RESULTS' named entity 'RNAS' named entity 'PORCINE CIRCOVIRUS TYPE 2' named entity 'INHIBITION' named entity 'AVAILABLE' named entity 'CELLS' named entity 'CAUSATIVE AGENT' named entity 'SIGNIFICANT' named entity 'IMMUNOHISTOCHEMICAL ANALYSES' named entity 'RNA INTERFERENCE' named entity 'TESTED' named entity 'USING' named entity 'POSSIBILITY OF' named entity 'TARGET' named entity 'APPROACH' named entity 'PORCINE CIRCOVIRUS TYPE 2' named entity 'PLASMID' named entity 'A MOUSE' named entity 'STRATEGY' named entity 'REDUCTION' named entity 'TRANSFECTION' named entity 'VIRAL DNA' named entity 'PRIMARY' named entity 'ANTIVIRAL TREATMENT' named entity 'THERAPEUTIC' named entity 'EFFECT' named entity 'INFECTION' named entity 'GENOME' named entity 'LEVEL' named entity 'RNA INTERFERENCE' named entity 'BORNE' named entity 'POSTWEANING MULTISYSTEMIC WASTING SYNDROME' named entity 'IN VIVO' named entity 'THESE' named entity 'SHORT' named entity 'GENERATED' named entity 'DISEASE' named entity 'SWINE DISEASE' named entity 'MICROSCOPIC' named entity 'VIRAL DNA REPLICATION' named entity 'treated'

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