About: The role of transmembrane protein 27 (TMEM27) in islet physiology and its potential use as a beta cell mass biomarker

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About: The role of transmembrane protein 27 (TMEM27) in islet physiology and its potential use as a beta cell mass biomarker Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The role of transmembrane protein 27 (TMEM27) in islet physiology and its potential use as a beta cell mass biomarker
Creator	Barbera, A Altirriba, J Barbera, : Casas, S Gasa, : Gasa, R Gomis, : Gomis, R Gutierrez-Dalmau, A Ramírez-Bajo, M Ros, S Ruiz De Villa, M
Source	PMC
abstract	AIMS/HYPOTHESIS: Transmembrane protein 27 (TMEM27) is a membrane protein cleaved and shed by pancreatic beta cells that has been proposed as a beta cell mass biomarker. Despite reports of its possible role in insulin exocytosis and cell proliferation, its function in beta cells remains controversial. We aimed to characterise the function of TMEM27 in islets and its potential use as a beta cell mass biomarker. METHODS: To determine TMEM27 function, we studied TMEM27 gene expression and localisation in human healthy and diabetic islets, the correlation of its expression with cell cycle and insulin secretion genes in human islets, its expression in tungstate-treated rats, and the effects of its overproduction on insulin secretion and proliferation in a beta cell line and islets. To elucidate its utility as a beta cell mass biomarker, we studied TMEM27 cleavage in a beta cell line, islets and primary proximal tubular cells. RESULTS: TMEM27 mRNA levels in islets are lower in diabetic donors than in controls. Its gene expression correlates with that of insulin and SNAPIN in human islets. TMEM27 expression is downregulated in islets of tungstate-treated rats, which exhibit decreased insulin secretion and increased proliferation. TMEM27 overproduction in a beta cell line and islets significantly enhanced glucose-induced insulin secretion, with modest or no effects on proliferation. Finally, TMEM27 is cleaved and shed by renal proximal tubular cells and pancreatic islets. CONCLUSIONS/INTERPRETATION: Our data support a role for TMEM27 in glucose-induced insulin secretion but not in cell proliferation. The finding that its cleavage is not specific to beta cells challenges the current support for its use as a potential beta cell mass biomarker. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00125-010-1728-6) contains supplementary material, which is available to authorised users.
has issue date	2010-04-13(xsd:dateTime)
bibo:doi	10.1007/s00125-010-1728-6
bibo:pmid	20386877
has license	no-cc
sha1sum (hex)	be9bdbb4987a83ad38fb0b65018528055e13eab7
schema:url	https://doi.org/10.1007/s00125-010-1728-6
resource representing a document's title	The role of transmembrane protein 27 (TMEM27) in islet physiology and its potential use as a beta cell mass biomarker
has PubMed Central identifier	PMC7096040
has PubMed identifier	20386877
schema:publication	Diabetologia
resource representing a document's body	covid:be9bdbb4987a83ad38fb0b65018528055e13eab7#body_text
is schema:about of	named entity 'mass' named entity 'human' named entity 'correlation' named entity 'beta cell' named entity 'correlates' named entity 'Despite' named entity 'donors' named entity 'beta cell' named entity 'PROXIMAL' named entity 'proliferation' named entity 'human' named entity 'function' named entity 'cells' named entity 'islets' named entity 'expression' named entity 'pancreatic islets' named entity 'insulin secretion' named entity 'proximal tubular' named entity 'pancreatic islets' named entity 'beta cell' named entity 'tungstate' named entity 'downregulated' named entity 'gene expression' named entity 'tungstate' named entity 'beta cell' named entity 'beta cell' named entity 'biomarker' named entity 'insulin secretion' named entity 'cleavage' named entity 'kDa' named entity 'tungstate' named entity 'cleavage' named entity 'biopsies' named entity 'mmol/l' named entity 'Billerica' named entity 'actin' named entity 'beta cell' named entity 'standard curve' named entity 'kidneys' named entity 'protein' named entity 'phenotype' named entity 'glucose homeostasis' named entity 'plasmid' named entity 'pancreatic islets' named entity 'insulin resistance' named entity 'rat' named entity 'pancreatic polypeptide' named entity 'cell type' named entity 'culture medium' named entity 'glucagon' named entity 'kDa' named entity 'posttranslational modifications' named entity 'glycosidase' named entity 'correlation' named entity 'cDNA' named entity 'Longitudinal data' named entity 'physiology' named entity 'myc' named entity 'supernatant' named entity 'pancreatic beta cell' named entity 'gene expression' named entity 'biomarker' named entity 'pancreatic beta cell' named entity 'beta cell' named entity 'protein' named entity 'mmol/l' named entity 'insulin secretion' named entity 'gain-of-function' named entity 'Barcelona' named entity 'antibodies'

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