About: Binding properties of a highly potent and selective iodinated aminopeptidase N inhibitor appropriate for radioautography

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About: Binding properties of a highly potent and selective iodinated aminopeptidase N inhibitor appropriate for radioautography Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Binding properties of a highly potent and selective iodinated aminopeptidase N inhibitor appropriate for radioautography
Creator	Bischo¡, Laurent Chen, Huixiong Da Nascimento, Sophie Fournië-Zaluski, Marie-Claude La\| «-Kuen, Renë Luciani, Nathalie Noble, Florence Roques, Bernard
Source	Elsevier; Medline; PMC
abstract	Aminopeptidase N (APN) is a zinc metallopeptidase involved in the inactivation of biologically active peptides. The knowledge of its precise distribution is crucial to investigate its physiological role. This requires the use of appropriate probes such as the recently developed highly potent and selective radiolabeled APN inhibitor 2(S)-benzyl-3-[hydroxy(1′(R)-aminoethyl)phosphinyl]propanoyl-L-3-[(125)I]iodotyrosine ([(125)I]RB 129). Its binding properties were investigated using rat brain homogenates (K(d)=3.4 nM) or APN expressed in COS-7 cells (K(d)=0.9 nM). The specific binding was 95% at [K(d)], and preliminary autoradiography in intestine is promising. The decreased affinity of [(125)I]RB 129 (=10(−6) M) for the E(350)D APN mutant, supports the critical role of E(350) in the amino-exopeptidase action of APN.
has issue date	2000-02-04(xsd:dateTime)
bibo:doi	10.1016/s0014-5793(99)01645-2
bibo:pmid	10664461
has license	no-cc
sha1sum (hex)	bea57182ab9a791a687d38d84bd6665b95c8ad7d
schema:url	https://doi.org/10.1016/s0014-5793%2899%2901645-2
resource representing a document's title	Binding properties of a highly potent and selective iodinated aminopeptidase N inhibitor appropriate for radioautography
has PubMed Central identifier	PMC7141570
has PubMed identifier	10664461
schema:publication	FEBS Lett
resource representing a document's body	covid:bea57182ab9a791a687d38d84bd6665b95c8ad7d#body_text
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