About: Influence of Mabs on PrP(Sc) Formation Using In Vitro and Cell-Free Systems

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About: Influence of Mabs on PrP(Sc) Formation Using In Vitro and Cell-Free Systems Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Influence of Mabs on PrP(Sc) Formation Using In Vitro and Cell-Free Systems
Creator	Chang, B Petersen, R Rubenstein, R Wisniewski, T
Source	PMC
abstract	PrP(Sc) is believed to serve as a template for the conversion of PrP(C) to the abnormal isoform. This process requires contact between the two proteins and implies that there may be critical contact sites that are important for conversion. We hypothesized that antibodies binding to either PrP(c)or PrP(Sc) would hinder or prevent the formation of the PrP(C)–PrP(Sc) complex and thus slow down or prevent the conversion process. Two systems were used to analyze the effect of different antibodies on PrP(Sc) formation: (i) neuroblastoma cells persistently infected with the 22L mouse-adapted scrapie stain, and (ii) protein misfolding cyclic amplification (PMCA), which uses PrP(Sc) as a template or seed, and a series of incubations and sonications, to convert PrP(C) to PrP(Sc). The two systems yielded similar results, in most cases, and demonstrate that PrP-specific monoclonal antibodies (Mabs) vary in their ability to inhibit the PrP(C)–PrP(Sc) conversion process. Based on the numerous and varied Mabs analyzed, the inhibitory effect does not appear to be epitope specific, related to PrP(C) conformation, or to cell membrane localization, but is influenced by the targeted PrP region (amino vs carboxy).
has issue date	2012-07-27(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0041626
bibo:pmid	22848548
has license	cc-by
sha1sum (hex)	bf0c3f90978f7e4794dfe1dd2d90ddb592fc6549
schema:url	https://doi.org/10.1371/journal.pone.0041626
resource representing a document's title	Influence of Mabs on PrP(Sc) Formation Using In Vitro and Cell-Free Systems
has PubMed Central identifier	PMC3407222
has PubMed identifier	22848548
schema:publication	PLoS One
resource representing a document's body	covid:bf0c3f90978f7e4794dfe1dd2d90ddb592fc6549#body_text
is schema:about of	named entity 'vary' named entity 'PrP' named entity 'inhibitory' named entity 'process' named entity 'prevent' named entity 'PrP' named entity 'FREE' named entity 'PRP' named entity 'INFLUENCE' named entity 'SYSTEMS' covid:arg/bf0c3f90978f7e4794dfe1dd2d90ddb592fc6549 named entity 'ADAPTED' named entity 'CONFORMATION' named entity 'IMPORTANT' named entity 'SLOW' named entity 'TO SERVE AS' named entity 'INFECTED' named entity 'TO INHIBIT' named entity 'RELATED' named entity 'SYSTEMS' named entity 'USING' named entity 'USED' named entity 'BUT' named entity 'MOUSE' named entity 'FORMATION' named entity 'CELL' named entity '28I' named entity 'LOCALIZATION' named entity 'COMPLEX' named entity 'SPECIFIC' named entity 'NUMEROUS' named entity 'CELL MEMBRANE' named entity 'TO CONVERT' named entity 'PRP' named entity 'BINDING' named entity 'DIFFERENT' named entity 'STAIN' named entity 'BASED' named entity 'ABNORMAL' named entity 'PROCESS' named entity 'ANALYZED' named entity 'EPITOPE' named entity 'SEED' named entity 'DOES NOT' named entity 'PERSISTENTLY' named entity 'CARBOXY' named entity 'SCRAPIE' named entity 'PROTEINS' named entity 'APPEAR' named entity 'AMPLIFICATION' named entity 'MAY BE' named entity 'PROTEIN MISFOLDING' named entity 'TEMPLATE' named entity 'CONTACT' named entity 'ANTIBODIES' named entity 'CONVERSION' named entity 'ISOFORM' named entity 'CYCLIC' named entity 'EITHER' named entity 'INHIBITORY EFFECT' named entity 'SIMILAR' named entity 'THEIR' named entity 'CRITICAL' named entity 'MONOCLONAL ANTIBODIES' named entity 'CASES' named entity 'NEUROBLASTOMA CELLS' named entity 'SITES' named entity 'SERIES' named entity 'USES' named entity 'FORMATION' named entity 'TARGETED' named entity 'SONICATIONS' named entity 'REGION' named entity 'IN VITRO' named entity 'EFFECT' named entity 'PREVENT' named entity 'RESULTS' named entity 'antibodies'

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