About: Gene cloning, sequencing, expression and biological activity of giant panda (Ailuropoda melanoleuca) interferon-α

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About: Gene cloning, sequencing, expression and biological activity of giant panda (Ailuropoda melanoleuca) interferon-α Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Gene cloning, sequencing, expression and biological activity of giant panda (Ailuropoda melanoleuca) interferon-α
Creator	Song, Hong-Mei Tan, Xue-Mei Tang, Yang Yang, Yun-Fei Zhang, Yi-Zheng
topic	covid:bf80f0ee336a378cca9d2e08d63e7a80ca85bd52#this
Source	Elsevier; Medline; PMC
abstract	Abstract The giant panda (Ailuropoda melanoleuca) is an endangered species and indigenous to China. In mammals, multiple subtypes of interferon-α (IFN-α) exist, most of which possess antiviral activity. Little is known about giant panda IFN-α genes and the role they may play in giant panda immunological responses to viruses. We have cloned genes encoding 12 giant panda IFN-α (AmIFN-α or AmIFNA) subtypes that share from 90 to 99% amino acid sequence identity. AmIFN-α12 has one additional amino acid at position 57, which is not present in other subtypes. Sequence identity of the AmIFN-α proteins encoded by the 12 genes compared to human IFN-α2 is approximately 58%. Unlike most of the human subtypes, each of the 12 giant panda IFN sequences has an N-glycosylation recognition site. Expression of all 12 AmIFN-α subtypes in 293 cells was confirmed by SDS-PAGE and Western blotting analysis. The antiviral activity and antiproliferative activity of each AmIFN-α subtype produced in transiently transfected 293 cell cultures were tested in vitro. All AmIFN-α subtypes were found to be stable at pH 2 or 65°C and to exhibit antiviral activity. Some IFN subtypes (AmIFN-α8 and AmIFN-α4) showed higher biological activity levels than others, whereas AmIFN-α11 exhibited lower activity. AmIFN-α had various antiproliferative activities to different target cells. To B16 cells, AmIFN-α3, AmIFN-α4, AmIFN-α8 had the highest activities, while to K562 cells, AmIFN-α3, AmIFN-α7, AmIFN-α10 had the highest activities. The various IFN-α subtypes displayed a good correlation between their antiviral and antiproliferative potencies.
has issue date	2007-04-30(xsd:dateTime)
bibo:doi	10.1016/j.molimm.2006.12.017
bibo:pmid	17346794
has license	els-covid
sha1sum (hex)	bf80f0ee336a378cca9d2e08d63e7a80ca85bd52
schema:url	https://doi.org/10.1016/j.molimm.2006.12.017
resource representing a document's title	Gene cloning, sequencing, expression and biological activity of giant panda (Ailuropoda melanoleuca) interferon-α
has PubMed Central identifier	PMC7112545
has PubMed identifier	17346794
schema:publication	Molecular Immunology
resource representing a document's body	covid:bf80f0ee336a378cca9d2e08d63e7a80ca85bd52#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/bf80f0ee336a378cca9d2e08d63e7a80ca85bd52
is schema:about of	named entity 'MELANOLEUCA' named entity 'additional' named entity 'Western blotting' named entity 'activity' named entity 'China' named entity 'subtypes' named entity 'INTERFERON' named entity 'HIGHER' named entity 'A32' named entity 'THEIR' named entity 'LITTLE' named entity 'POSITION' named entity 'GOOD' named entity 'ANTIVIRAL ACTIVITY' named entity 'AMINO ACID' named entity 'MELANOLEUCA' named entity 'FOUND' named entity 'COMPARED' named entity 'MOST OF' named entity 'CLONED GENES' named entity 'KNOWN' named entity 'DIFFERENT' named entity 'ACTIVITIES' named entity 'APPROXIMATELY' named entity 'ANTIVIRAL' named entity 'DISPLAYED' named entity 'ENDANGERED SPECIES' named entity 'SITE' named entity 'GENES' named entity 'SHARE' named entity 'IMMUNOLOGICAL' named entity 'STABLE' named entity 'MAMMALS' named entity 'EXIST' named entity 'SDS-PAGE' named entity '80%' named entity '293' named entity 'A38' named entity 'TARGET CELLS' named entity 'ENCODED' named entity 'SUBTYPE' named entity 'A34' named entity 'IN VITRO' named entity 'VARIOUS' named entity 'ACTIVITY LEVELS' named entity 'OF EACH' named entity 'ENCODING' named entity 'A33' named entity 'CELL CULTURES' named entity '90%' named entity 'POSSESS' named entity 'INTERFERON' named entity 'ACTIVITY' named entity 'RESPONSES' named entity 'CONFIRMED BY' named entity 'N-GLYCOSYLATION' named entity 'EXPRESSION' named entity 'ABOUT' named entity 'SUBTYPES' named entity 'AMINO ACID SEQUENCE' covid:arg/bf80f0ee336a378cca9d2e08d63e7a80ca85bd52 named entity '293 CELL' named entity 'CORRELATION' named entity 'HAVE' named entity 'BIOLOGICAL ACTIVITY' named entity 'TESTED' named entity 'EXHIBIT' named entity 'GIANT PANDA' named entity 'GENE CLONING' named entity 'EXPRESSION' named entity 'SEQUENCING' named entity 'ADDITIONAL' named entity 'PLAY' named entity 'PROTEINS' named entity 'A37'

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