About: Detection of Group A Beta Hemolytic Streptococci Species, emm, and Exotoxin Genes Isolated from Patients with Tonsillopharyngitis

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About: Detection of Group A Beta Hemolytic Streptococci Species, emm, and Exotoxin Genes Isolated from Patients with Tonsillopharyngitis Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection of Group A Beta Hemolytic Streptococci Species, emm, and Exotoxin Genes Isolated from Patients with Tonsillopharyngitis
Creator	Altun, Mehzat Binnur, · Yapıcı, Mericli
Source	PMC
abstract	Group A Beta Hemolytic Streptococci (GAS) is the most critical human pathogen that leads to tonsillopharyngitis. The aims of this study were to identify GAS isolates and to determine emm typing, the coverage rate of available vaccines, and the distribution of superantigen gene profiles. 15 GAS isolates were isolated from throat cultures of 200 patients with tonsillopharyngitis, who were admitted to Canakkale Health Application and Research Hospital between October 2017 and May 2018. Identification of the isolates was performed by conventional methods and 16S rRNA sequence analysis. emm typing and exotoxin profiling of the isolates were performed by polymerase chain reaction. 7.5% GAS was detected in 200 patients. All the GAS isolates were identified as S. pyogenes. emm typing can be carried out in 13 S. pyogenes isolates. emm89 (33.3%), emm44 (20%), emm6 (13.3%), emm84 (6.7%), emm1 (6.7%), and emm18.1 (6.7%) were found to be six emm types. The coverage rate of S. pyogenes strains for 26-valent vaccine was 61.5% and for the 30-valent vaccine 84.6%. The most common exotoxin was speB (86.7%), followed by speC (60%), speF (33.3%), ssa (26.7%), speA (20%), speM (20%), speJ (13.3%), speL (6.7%), and speI (6.7%). As a result of determining the emm types of S. pyogenes species in Canakkale, it was concluded that the potential of 30-valent vaccine should be considered in Turkey and development of vaccines containing exotoxin types may be beneficial.
has issue date	2020-05-04(xsd:dateTime)
bibo:doi	10.1007/s00284-020-01994-5
bibo:pmid	32367278
has license	no-cc
sha1sum (hex)	bf881abd5bdf473c548ac22834cab2ac9b3b375b
schema:url	https://doi.org/10.1007/s00284-020-01994-5
resource representing a document's title	Detection of Group A Beta Hemolytic Streptococci Species, emm, and Exotoxin Genes Isolated from Patients with Tonsillopharyngitis
has PubMed Central identifier	PMC7222056
has PubMed identifier	32367278
schema:publication	Curr Microbiol
resource representing a document's body	covid:bf881abd5bdf473c548ac22834cab2ac9b3b375b#body_text
is schema:about of	named entity 'emm' named entity 'strains' named entity 'isolated' named entity 'May' named entity 'coverage' named entity 'S. pyogenes' named entity 'Detection' named entity 'Group A' named entity 'RESEARCH' named entity 'COVERAGE' named entity 'FOLLOWED BY' named entity 'STRAINS' named entity 'LEADS' named entity 'SPEJ' named entity '16S RRNA' named entity 'ADMITTED TO' named entity 'HEALTH' named entity 'SPECIES' named entity 'TYPING' named entity 'COMMON' named entity 'VACCINE' named entity 'SUPERANTIGEN' named entity 'EMM' named entity 'CULTURES' named entity 'STUDY' named entity 'ISOLATES' named entity 'S. pyogenes' named entity 'polymerase' named entity 'human pathogen' named entity 'conventional' named entity 'profiles' named entity 'gene' named entity 'GAS' named entity 'common' named entity 'coverage' named entity 'patients' named entity '2018' named entity 'Canakkale' named entity 'identified' named entity 'Streptococci' named entity 'S. pyogenes' named entity 'S. pyogenes' named entity 'throat cultures' named entity '20%' named entity 'Streptococci' named entity 'speM' named entity 'exotoxin' named entity 'Hemolytic' named entity 'human pathogen' named entity 'exotoxin' named entity 'gene' named entity 'Streptococci' named entity 'Exotoxin' named entity 'Tonsillopharyngitis' named entity 'determine' named entity 'public health' named entity 'Taq polymerase' named entity 'S. pyogenes' named entity 'pathogen' named entity 'Turkey' named entity 'Turkey' named entity 'India' named entity 'gene' named entity 'variable regions' named entity 'exotoxin' named entity 'S. pyogenes' named entity 'acute rheumatic fever' named entity 'multivalent vaccine' named entity 'primers' named entity 'exotoxin' named entity 'S. pyogenes' named entity 'PCR' named entity 'low-income countries' named entity 'S. pyogenes' named entity 'exotoxins' named entity 'throat cultures' named entity 'DNA sequences' named entity 'PCR' named entity '16S rRNA'

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