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About:
An immunoassay cassette with a handheld reader for HIV urine testing in point-of-care diagnostics
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Academic Article
research paper
schema:ScholarlyArticle
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type
Academic Article
research paper
schema:ScholarlyArticle
isDefinedBy
Covid-on-the-Web dataset
has title
An immunoassay cassette with a handheld reader for HIV urine testing in point-of-care diagnostics
Creator
Wang, Dong
Xia, Ningshao
Zhang, Xiaolei
Ge, Shengxiang
Liu, Luyao
Qiu, Xianbo
Dong, Xiaobing
Duli, &
Gong, Shisong
Yang, Dianlong
Yang, Wenbo
Yu, Shengda
Source
PMC
abstract
Currently, most HIV tests are performed with blood samples, or alternatively saliva samples are used for HIV testing. Simple HIV tests need to be performed in hospitals or other medical agencies instead of more invasive HIV blood tests. To enable point-of-care (POC) HIV diagnostics, based on a recently developed lateral flow strip for HIV urine testing, a microfluidic immunoassay cassette with a handheld optical reader is developed. Based on lateral flow strip with gold colloid reporter, the integrated immunoassay cassette can perform sample introduction, metering, discharging, applying and detection which simplifies HIV testing. An indicator is incorporated into the cassette to guide sample introduction based on color change, and further, the excess test sample is stored inside the sealed cassette to avoid any contamination. The low-cost handheld optical reader can provide a test result within a few seconds, which is useful for simple, sensitive and affordable HIV onsite detection. Instead of using normal white LEDs, a customized back light module embedded with green LEDs is adopted to illuminate the lateral flow strip with an appropriate working current to achieve optimal performance. Compared to the standard lateral flow strips using a benchtop reader, with the disposable immunoassay cassette assisted by the handheld optical reader, more convenient, easier-to-operate, and more affordable HIV urine testing can be achieved in POC diagnostics.
has issue date
2020-05-21
(
xsd:dateTime
)
bibo:doi
10.1007/s10544-020-00494-4
bibo:pmid
32436002
has license
no-cc
sha1sum (hex)
c0197686ca77c46984000c70f8a038df3073d0fa
schema:url
https://doi.org/10.1007/s10544-020-00494-4
resource representing a document's title
An immunoassay cassette with a handheld reader for HIV urine testing in point-of-care diagnostics
has PubMed Central identifier
PMC7239691
has PubMed identifier
32436002
schema:publication
Biomed Microdevices
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covid:c0197686ca77c46984000c70f8a038df3073d0fa#body_text
is
schema:about
of
named entity 'immunoassay'
named entity 'Activities of Daily Living'
named entity 'lateral flow'
named entity 'HIV positive'
named entity 'urine sample'
named entity 'Shenzhen'
named entity 'Biological Pharmacy'
named entity 'urine testing'
named entity 'lateral flow'
named entity 'nucleic acids'
named entity 'microfluidic'
named entity 'immunoassay'
named entity 'SPA'
named entity 'point of care'
named entity 'micro-controller'
named entity 'lateral flow'
named entity 'HIV test'
named entity 'CMOS camera'
named entity 'immunoassay'
named entity 'red blood cell'
named entity 'optical reader'
named entity 'antibodies'
named entity 'Protein'
named entity 'Photoelectricity'
named entity 'Biotech'
named entity 'antibody'
named entity 'immunoassay'
named entity 'PMMA'
named entity 'lateral flow'
named entity 'optical reader'
named entity 'HIV'
named entity 'antibodies'
named entity 'SPA'
named entity 'protein'
named entity 'Shenzhen'
named entity 'clinical trials'
named entity 'lateral flow immunoassay'
named entity 'PTFE'
named entity 'lateral flow'
named entity 'PMMA'
named entity 'optical reader'
named entity 'gold colloid'
named entity 'optical reader'
named entity 'HIV antibody'
named entity 'optical reader'
named entity 'urine sample'
named entity 'China'
named entity 'HIV'
named entity 'urine testing'
named entity 'HIV'
named entity 'urine samples'
named entity 'lateral flow'
named entity 'point-of-care diagnostics'
named entity 'optical reader'
named entity 'lateral flow'
named entity 'immunoassay'
named entity 'microcontroller'
named entity 'immunoassay'
named entity 'Jinan'
named entity 'incubation'
named entity 'Acquired Immune Deficiency Syndrome'
named entity 'BSA'
named entity 'HIV'
named entity 'phosphor'
named entity 'urine sample'
named entity 'urine'
named entity 'gold colloid'
named entity 'urine samples'
named entity 'microfluidic'
named entity 'urine tests'
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