About: Subcellular location and topology of severe acute respiratory syndrome coronavirus envelope protein

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Subcellular location and topology of severe acute respiratory syndrome coronavirus envelope protein
Creator	Enjuanes, Luis Kremer, Leonor Llorente, Mercedes Shuo, Shen Dediego, Marta Jiménez-Guardeño, Jose Nieto-Torres, Jose Regla-Nava, Jose
Source	Elsevier; Medline; PMC
abstract	Abstract Severe acute respiratory syndrome (SARS) coronavirus (CoV) envelope (E) protein is a transmembrane protein. Several subcellular locations and topological conformations of E protein have been proposed. To identify the correct ones, polyclonal and monoclonal antibodies specific for the amino or the carboxy terminus of E protein, respectively, were generated. E protein was mainly found in the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) of cells transfected with a plasmid encoding E protein or infected with SARS-CoV. No evidence of E protein presence in the plasma membrane was found by using immunofluorescence, immunoelectron microscopy and cell surface protein labeling. In addition, measurement of plasma membrane voltage gated ion channel activity by whole-cell patch clamp suggested that E protein was not present in the plasma membrane. A topological conformation in which SARS-CoV E protein amino terminus is oriented towards the lumen of intracellular membranes and carboxy terminus faces cell cytoplasm is proposed.
has issue date	2011-07-05(xsd:dateTime)
bibo:doi	10.1016/j.virol.2011.03.029
bibo:pmid	21524776
has license	els-covid
sha1sum (hex)	c06616024672d10c2192434d63cfd1895a31aa40
schema:url	https://doi.org/10.1016/j.virol.2011.03.029
resource representing a document's title	Subcellular location and topology of severe acute respiratory syndrome coronavirus envelope protein
has PubMed Central identifier	PMC4726981
has PubMed identifier	21524776
schema:publication	Virology
resource representing a document's body	covid:c06616024672d10c2192434d63cfd1895a31aa40#body_text
is schema:about of	named entity 'envelope' covid:arg/c06616024672d10c2192434d63cfd1895a31aa40 named entity 'severe acute respiratory syndrome coronavirus' named entity 'envelope protein' named entity 'protein' named entity 'Cell Surface Protein' named entity 'Western blot' named entity 'ERGIC' named entity 'hybridomas' named entity 'IgM' named entity 'protein' named entity 'protein translation' named entity 'lysines' named entity 'Western blot' named entity 'Immunofluorescence' named entity 'glutaraldehyde' named entity 'immunogenic' named entity 'cadherin' named entity 'monovalent' named entity 'intracellular' named entity 'carboxy terminus' named entity 'cell surface protein' named entity 'peptide' named entity 'antibodies' named entity 'antibody' named entity 'biotinylation' named entity 'ion channel' named entity 'immunoglobulin' named entity 'SARS-CoV' named entity 'secretion' named entity 'carboxy terminal' named entity 'intracellular' named entity 'intracellular' named entity 'transfection' named entity 'virulence factor' named entity 'ion channels' named entity 'virus' named entity 'protein' named entity 'biotinylation' named entity 'SARS-CoV' named entity 'hybridoma' named entity 'carboxy terminal' named entity 'polyacrylamide gel electrophoresis' named entity 'Vero E6' named entity 'Axon' named entity 'protein' named entity 'plasmid' named entity 'protein' named entity 'luminal' named entity 'oligopeptide' named entity 'viral factories' named entity 'SARS-CoV' named entity 'intrinsic activities' named entity 'mAbs' named entity 'plasma membrane' named entity 'plasma membrane' named entity 'mice' named entity 'protein topology' named entity 'ion channel' named entity 'virus' named entity 'SARS' named entity 'protein' named entity 'protein' named entity 'protein' named entity 'cadherin' named entity 'African green monkey' named entity 'immunofluorescence' named entity 'cation' named entity 'nucleoprotein' named entity '12 nm' named entity 'amino terminal' named entity 'intracellular' named entity 'protein' named entity 'protein'

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