About: Differential proteomic analysis of virus-enriched fractions obtained from plasma pools of patients with dengue fever or severe dengue

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About: Differential proteomic analysis of virus-enriched fractions obtained from plasma pools of patients with dengue fever or severe dengue Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Differential proteomic analysis of virus-enriched fractions obtained from plasma pools of patients with dengue fever or severe dengue
Creator	Buchy, Philippe Paranhos-Baccala, Glaucia Bedin, Frederic Duong, Vasna Flamand, Marie Fragnoud, Romain Pachot, Alexandre Reynier, Frederic
Source	Medline; PMC
abstract	BACKGROUND: Dengue is the most widespread mosquito-borne viral disease of public health concern. In some patients, endothelial cell and platelet dysfunction lead to life-threatening hemorrhagic dengue fever or dengue shock syndrome. Prognostication of disease severity is urgently required to improve patient management. The pathogenesis of severe dengue has not been fully elucidated, and the role of host proteins associated with viral particles has received little exploration. METHODS: The proteomes of virion-enriched fractions purified from plasma pools of patients with dengue fever or severe dengue were compared. Virions were purified by ultracentrifugation combined with a water-insoluble polyelectrolyte-based technique. Following in-gel hydrolysis, peptides were analyzed by nano-liquid chromatography coupled to ion trap mass spectrometry and identified using data libraries. RESULTS: Both dengue fever and severe dengue viral-enriched fractions contained identifiable viral envelope proteins and host cellular proteins. Canonical pathway analysis revealed the identified host proteins are mainly involved in the coagulation cascade, complement pathway or acute phase response signaling pathway. Some host proteins were over- or under-represented in plasma from patients with severe dengue compared to patients with dengue fever. ELISAs were used to validate differential expression of a selection of identified host proteins in individual plasma samples of patients with dengue fever compared to patients with severe dengue. Among 22 host proteins tested, two could differentiate between dengue fever and severe dengue in two independent cohorts (olfactomedin-4: area under the curve (AUC), 0.958; and platelet factor-4: AUC, 0.836). CONCLUSION: A novel technique of virion-enrichment from plasma has allowed to identify two host proteins that have prognostic value for classifying patients with acute dengue who are more likely to develop a severe dengue. The impact of these host proteins on pathogenicity and disease outcome are discussed. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12879-015-1271-7) contains supplementary material, which is available to authorized users.
has issue date	2015-11-14(xsd:dateTime)
bibo:doi	10.1186/s12879-015-1271-7
bibo:pmid	26572220
has license	cc-by
sha1sum (hex)	c0bec3dd80af76e0f60020b72ab8f6e1f14354fb
schema:url	https://doi.org/10.1186/s12879-015-1271-7
resource representing a document's title	Differential proteomic analysis of virus-enriched fractions obtained from plasma pools of patients with dengue fever or severe dengue
has PubMed Central identifier	PMC4647599
has PubMed identifier	26572220
schema:publication	BMC Infect Dis
resource representing a document's body	covid:c0bec3dd80af76e0f60020b72ab8f6e1f14354fb#body_text
is schema:about of	named entity 'PATIENTS' named entity 'platelet' named entity 'Virions' named entity 'acute phase response' named entity 'particles' named entity 'dysfunction' named entity 'The' named entity 'host' named entity 'mass spectrometry' named entity 'proteomic' named entity 'plasma' named entity 'VIRAL-' named entity 'PATIENTS' named entity 'ROLE' named entity 'CONCLUSION' named entity 'FRACTIONS' named entity 'WIDESPREAD' named entity 'HYDROLYSIS' named entity 'USED' named entity 'WATER-INSOLUBLE' named entity 'CLASSIFYING' named entity 'MOSQUITO' named entity 'BORNE' covid:arg/c0bec3dd80af76e0f60020b72ab8f6e1f14354fb named entity 'INDIVIDUAL' named entity 'RECEIVED' named entity 'THESE' named entity 'USING' named entity 'BASED' named entity 'PLATELET DYSFUNCTION' named entity 'PATIENT MANAGEMENT' named entity 'CONTAINED' named entity 'ALLOWED' named entity 'FOLLOWING' named entity 'VIRIONS' named entity 'CELLULAR' named entity 'POOLS' named entity 'PLATELET FACTOR-4' named entity 'PLASMA' named entity 'VIRUS' named entity 'DENGUE FEVER' named entity 'DIFFERENTIAL PROTEOMIC ANALYSIS' named entity 'OBTAINED' named entity 'SEVERE DENGUE' named entity 'PURIFIED' named entity 'VIRAL DISEASE' named entity 'LIQUID CHROMATOGRAPHY ' named entity 'PUBLIC HEALTH' named entity 'EXPLORATION' named entity 'PROTEINS' named entity 'COMPARED' named entity 'DENGUE' named entity 'AREA UNDER THE CURVE' named entity 'HOST' named entity 'REQUIRED' named entity 'SEVERE DENGUE' named entity 'ULTRACENTRIFUGATION' named entity 'ACUTE' named entity 'COAGULATION' named entity 'ASSOCIATED WITH' named entity 'NANO-' named entity 'SAMPLES' named entity 'ELISAS' named entity 'LIBRARIES' named entity 'PATHOGENESIS' named entity 'ENRICHED' named entity 'METHODS' named entity 'DATA' named entity 'PROGNOSTIC VALUE' named entity 'DIFFERENTIATE' named entity 'BACKGROUND' named entity 'HAVE' named entity 'CONCERN' named entity 'ENDOTHELIAL CELL'

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