About: An indirect enzyme-linked immunosorbent assay for the identification of antibodies to Senecavirus A in swine

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About: An indirect enzyme-linked immunosorbent assay for the identification of antibodies to Senecavirus A in swine Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	An indirect enzyme-linked immunosorbent assay for the identification of antibodies to Senecavirus A in swine
Creator	Murtaugh, Michael Vannucci, Fabio Stone, Suzanne Akkutay-Yoldar, Zeynep Tousignant, Steven Dvorak, Cheryl
Source	Medline; PMC
abstract	BACKGROUND: Senecavirus A (SVA), a member of the family Picornaviridae, genus Senecavirus, is a recently identified single-stranded RNA virus closely related to members of the Cardiovirus genus. SVA was originally identified as a cell culture contaminant and was not associated with disease until 2007 when it was first observed in pigs with Idiopathic Vesicular Disease (IVD). Vesicular disease is sporadically observed in swine, is not debilitating, but is significant due to its resemblance to foreign animal diseases, such as foot-and-mouth disease (FMD), whose presence would be economically devastating to the United States. IVD disrupts swine production until foreign animal diseases can be ruled out. Identification and characterization of SVA as a cause of IVD will help to quickly rule out infection by foreign animal diseases. METHODS: We have developed and characterized an indirect ELISA assay to specifically identify serum antibodies to SVA. Viral protein 1, 2 and 3 (VP1, VP2, VP3) were expressed, isolated, and purified from E. coli and used to coat plates for an indirect ELISA. Sera from pigs with and without IVD symptoms as well as a time course following animals from an infected farm, were analyzed to determine the antibody responses to VP1, VP2, and VP3. RESULTS: Antibody responses to VP2 were higher than VP1 and VP3 and showed high affinity binding on an avidity ELISA. ROC analysis of the SVA VP2 ELISA showed a sensitivity of 94.2% and a specificity of 89.7%. Compared to IFA, the quantitative ELISA showed an 89% agreement in negative samples and positive samples from 4–60 days after appearance of clinical signs. Immune sera positive for FMDV, encephalomyocarditis virus, and porcine epidemic diarrhea virus antibodies did not cross-react. CONCLUSIONS: A simple ELISA based on detection of antibodies to SVA VP2 will help to differentially diagnose IVD due to SVA and rule out the presence of economically devastating foreign animal diseases.
has issue date	2017-02-15(xsd:dateTime)
bibo:doi	10.1186/s12917-017-0967-x
bibo:pmid	28202026
has license	cc-by
sha1sum (hex)	c0de9d0fcd1d7f778c11f42fd399f6c0cc5e7a4b
schema:url	https://doi.org/10.1186/s12917-017-0967-x
resource representing a document's title	An indirect enzyme-linked immunosorbent assay for the identification of antibodies to Senecavirus A in swine
has PubMed Central identifier	PMC5312445
has PubMed identifier	28202026
schema:publication	BMC Vet Res
resource representing a document's body	covid:c0de9d0fcd1d7f778c11f42fd399f6c0cc5e7a4b#body_text
is schema:about of	named entity 'genus' named entity 'Picornaviridae' named entity 'members' named entity 'Antibody' named entity 'samples' named entity 'swine' named entity 'SVA' named entity 'production' named entity 'SVA' named entity 'indirect' covid:arg/c0de9d0fcd1d7f778c11f42fd399f6c0cc5e7a4b named entity 'disease' named entity 'IFA' named entity 'symptoms' named entity 'SVA' named entity 'developed' named entity 'based' named entity 'animal diseases' named entity 'positive' named entity 'positive' named entity 'animal diseases' named entity 'IVD' named entity 'Disease' named entity 'RNA virus' named entity 'observed' named entity 'E. coli' named entity 'observed' named entity 'antibodies' named entity 'diagnose' named entity 'SVA' named entity 'cross-react' named entity 'clinical signs' named entity 'IVD' named entity 'Immune sera' named entity 'IVD' named entity 'VP1' named entity 'FMDV' named entity 'swine production' named entity 'enzyme-linked immunosorbent assay' named entity 'swine production' named entity 'seropositive' named entity 'encephalomyocarditis virus' named entity 'virus' named entity 'seropositive' named entity 'positive control' named entity 'VP3' named entity 'clinical signs' named entity 'infection' named entity 'IFA test' named entity 'antigen' named entity 'FMD' named entity 'FMDV' named entity 'swine' named entity 'VP1' named entity 'total protein' named entity 'absorbance' named entity 'inoculated' named entity 'serotype' named entity 'infection' named entity 'seropositive' named entity 'FMDV' named entity 'EMCV' named entity 'area under the curve' named entity 'VP3' named entity 'infection' named entity '37°C' named entity 'enzyme-linked immunosorbent assay' named entity 'serum samples' named entity 'Bio-Rad Laboratories' named entity 'false positive' named entity 'antibodies' named entity 'animal diseases' named entity 'antibodies' named entity 'IgG' named entity 'PBS' named entity 'cobalt'

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