About: The cellular protein hnRNP A2/B1 enhances HIV-1 transcription by unfolding LTR promoter G-quadruplexes

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About: The cellular protein hnRNP A2/B1 enhances HIV-1 transcription by unfolding LTR promoter G-quadruplexes Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The cellular protein hnRNP A2/B1 enhances HIV-1 transcription by unfolding LTR promoter G-quadruplexes
Creator	Palù, Giorgio Ruggiero, Emanuela Frasson, Ilaria Lago, Sara Perrone, Rosalba Scalabrin, Matteo Tosoni, Elena Tassinari, Martina Richter, Sara
Source	Medline; PMC
abstract	G-quadruplexes are four-stranded conformations of nucleic acids that act as cellular epigenetic regulators. A dynamic G-quadruplex forming region in the HIV-1 LTR promoter represses HIV-1 transcription when in the folded conformation. This activity is enhanced by nucleolin, which induces and stabilizes the HIV-1 LTR G-quadruplexes. In this work by a combined pull-down/mass spectrometry approach, we consistently found hnRNP A2/B1 as an additional LTR-G-quadruplex interacting protein. Surface plasmon resonance confirmed G-quadruplex specificity over linear sequences and fluorescence resonance energy transfer analysis indicated that hnRNP A2/B1 is able to efficiently unfold the LTR G-quadruplexes. Evaluation of the thermal stability of the LTR G-quadruplexes in different-length oligonucleotides showed that the protein is fit to be most active in the LTR full-length environment. When hnRNP A2/B1 was silenced in cells, LTR activity decreased, indicating that the protein acts as a HIV-1 transcription activator. Our data highlight a tightly regulated control of transcription based on G-quadruplex folding/unfolding, which depends on interacting cellular proteins. These findings provide a deeper understanding of the viral transcription mechanism and may pave the way to the development of drugs effective against the integrated HIV-1, present both in actively and latently infected cells.
has issue date	2017-03-24(xsd:dateTime)
bibo:doi	10.1038/srep45244
bibo:pmid	28338097
has license	cc-by
sha1sum (hex)	c0fcd483132e1e59571bae716303dc7d3977b7d6
schema:url	https://doi.org/10.1038/srep45244
resource representing a document's title	The cellular protein hnRNP A2/B1 enhances HIV-1 transcription by unfolding LTR promoter G-quadruplexes
has PubMed Central identifier	PMC5364415
has PubMed identifier	28338097
schema:publication	Sci Rep
resource representing a document's body	covid:c0fcd483132e1e59571bae716303dc7d3977b7d6#body_text
is schema:about of	named entity 'ACTIVE' named entity 'VIRAL TRANSCRIPTION' named entity 'PROTEINS' named entity 'HIV-1' named entity 'EFFECTIVE' named entity 'TRANSCRIPTION' named entity 'PROVIDE' named entity 'INTERACTING' named entity 'OUR' named entity 'CELLS' named entity 'THESE' named entity 'DRUGS' named entity 'EVALUATION' named entity 'FOLDING' named entity 'fit' named entity 'data' named entity 'Evaluation' named entity 'resonance' named entity 'G-quadruplex' named entity 'transcription activator' named entity 'transcription' named entity 'OPEN' named entity 'BASED' named entity 'FINDINGS' named entity 'LTR' named entity 'DEVELOPMENT' named entity 'DIFFERENT' named entity 'INFECTED' named entity 'OLIGONUCLEOTIDES' named entity 'ENVIRONMENT' named entity 'G-QUADRUPLEX' named entity 'G-QUADRUPLEXES' named entity 'MECHANISM' named entity 'FLUORESCENCE RESONANCE ENERGY TRANSFER ' named entity 'CONFIRMED' named entity 'PROTEIN ' named entity 'CONTROL' named entity 'TRANSCRIPTION ACTIVATOR' named entity 'HIV-1' named entity 'PROMOTER' covid:arg/c0fcd483132e1e59571bae716303dc7d3977b7d6 named entity 'CELLULAR' named entity 'PROTEIN ' named entity 'TRANSCRIPTION' named entity 'LENGTH' named entity 'CELLULAR' named entity 'INDICATING' named entity 'FIT' named entity 'SURFACE PLASMON RESONANCE' named entity 'ANALYSIS' named entity 'ACTIVITY' named entity 'SPECIFICITY' named entity 'HNRNP A2' named entity 'DECREASED' named entity 'WAY' named entity 'PAVE' named entity 'HNRNP A2' named entity 'G-QUADRUPLEXES' named entity 'UNFOLDING' named entity 'LTR' named entity 'UNDERSTANDING' named entity 'REGULATED' named entity 'LINEAR' named entity 'DATA' named entity 'INTEGRATED' named entity 'ACTS' named entity 'THERMAL STABILITY' named entity 'SEQUENCES' named entity 'SILENCED' named entity 'PRESENT' named entity 'INDICATED' named entity 'deeper understanding' named entity 'interacting'

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