About: The Calcium-Sensing Receptor Regulates Plasma Membrane Calcium Adenosine Triphosphatase Isoform 2 Activity in Mammary Epithelial Cells: A Mechanism for Calcium-Regulated Calcium Transport into Milk

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About: The Calcium-Sensing Receptor Regulates Plasma Membrane Calcium Adenosine Triphosphatase Isoform 2 Activity in Mammary Epithelial Cells: A Mechanism for Calcium-Regulated Calcium Transport into Milk Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The Calcium-Sensing Receptor Regulates Plasma Membrane Calcium Adenosine Triphosphatase Isoform 2 Activity in Mammary Epithelial Cells: A Mechanism for Calcium-Regulated Calcium Transport into Milk
Creator	Neville, Margaret Vanhouten, Joshua Wysolmerski, John
topic	covid:c1537ba0e1eb2b64f85f45367f16dc4e15d566a3#this
Source	PMC
abstract	The calcium-sensing receptor (CaR) regulates transepithelial calcium transport into milk by mammary epithelial cells. Using a genome-wide screening strategy, we identified the plasma membrane calcium ATPase isoform 2 (PMCA2) as a potential downstream target of the CaR. We show that PMCA2 expression in the mouse mammary gland increases during lactation and that PMCA2 is localized solely to the apical plasma membrane of mammary epithelial cells. In milk from deafwaddler mice, which have mutations in the gene encoding PMCA2, calcium concentrations were reduced, confirming its importance in calcium transport into milk. Furthermore, in cultured primary and EpH4 mouse mammary epithelial cells, CaR stimulation up-regulated calcium-dependent ATPase activity in plasma membrane preparations. By small interfering RNA-mediated gene knockdown of PMCA2, we show that PMCA2 accounts for the preponderance of calcium-ATPase activity. We also show that reduction of CaR expression with small interfering RNA eliminates the ability of extracellular calcium to elicit an increase in calcium-dependent ATPase activity in EpH4 cell membranes. These results demonstrate that activation of the CaR increases PMCA2 activity in mouse mammary epithelial cells, providing a mechanism for the regulation of transepithelial calcium transport by calcium in the lactating mouse mammary gland.
has issue date	2007-12-01(xsd:dateTime)
bibo:doi	10.1210/en.2007-0850
bibo:pmid	17823248
has license	no-cc
sha1sum (hex)	c1537ba0e1eb2b64f85f45367f16dc4e15d566a3
schema:url	https://doi.org/10.1210/en.2007-0850
resource representing a document's title	The Calcium-Sensing Receptor Regulates Plasma Membrane Calcium Adenosine Triphosphatase Isoform 2 Activity in Mammary Epithelial Cells: A Mechanism for Calcium-Regulated Calcium Transport into Milk
has PubMed Central identifier	PMC7108505
has PubMed identifier	17823248
schema:publication	Endocrinology
resource representing a document's body	covid:c1537ba0e1eb2b64f85f45367f16dc4e15d566a3#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/c1537ba0e1eb2b64f85f45367f16dc4e15d566a3
is schema:about of	named entity 'MECHANISM' named entity 'The' named entity 'epithelial cells' named entity 'milk' named entity 'calcium' named entity 'calcium' named entity 'plasma membrane' named entity 'gene' named entity 'small interfering RNA' named entity 'milk' named entity 'apical' named entity 'mouse' named entity 'mammary gland' named entity 'Adenosine' named entity 'ATPASE ACTIVITY' named entity 'WIDE' named entity 'GENOME' named entity 'RESULTS' named entity 'ELICIT' named entity 'EXTRACELLULAR CALCIUM' named entity 'HAVE' named entity 'ATPASE' named entity 'REDUCTION' named entity 'APICAL PLASMA MEMBRANE' named entity 'REGULATION' named entity 'LACTATING' named entity 'GENE' named entity 'EXPRESSION' named entity 'PROVIDING' named entity 'SMALL INTERFERING RNA' named entity '5954' named entity 'REDUCED' named entity 'ACTIVITY' named entity 'EPITHELIAL CELLS' named entity 'MOUSE MAMMARY GLAND' named entity 'DOWNSTREAM' named entity 'MECHANISM' named entity 'CONFIRMING' named entity 'CALCIUM-DEPENDENT ATPASE ACTIVITY' covid:arg/c1537ba0e1eb2b64f85f45367f16dc4e15d566a3 named entity 'CALCIUM' named entity 'REGULATED' named entity 'IDENTIFIED' named entity 'ADENOSINE TRIPHOSPHATASE' named entity 'ISOFORM' named entity 'MAMMARY' named entity 'PLASMA MEMBRANE' named entity 'CALCIUM-SENSING RECEPTOR' named entity 'CALCIUM-SENSING RECEPTOR' named entity 'STRATEGY' named entity 'THESE' named entity 'DEPENDENT' named entity 'UP-REGULATED' named entity 'PRIMARY' named entity 'POTENTIAL' named entity '148' named entity 'MAMMARY' named entity 'LACTATION' named entity 'CELL MEMBRANES' named entity 'MOUSE' named entity 'CULTURED' named entity 'PLASMA MEMBRANE CALCIUM ATPASE ISOFORM 2' named entity 'ENCODING' named entity 'STIMULATION' named entity 'PMCA2' named entity 'TARGET' named entity 'USING' named entity 'ACTIVITY' named entity 'ABILITY' named entity 'ACTIVATION' named entity 'LOCALIZED' named entity 'SCREENING' named entity 'INCREASE' named entity 'GENE KNOCKDOWN' named entity 'MUTATIONS' named entity 'ITS' named entity 'MILK'

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