About: Efficient Qualitative and Quantitative Determination of Antigen-induced Immune Responses

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Efficient Qualitative and Quantitative Determination of Antigen-induced Immune Responses
Creator	Frego, Lee Kroe-Barrett, Rachel Lasaro, Marcio Singh, Sanjaya Truncali, Kristopher Yang, Danlin
Source	PMC
abstract	To determine the effectiveness of immunization strategies used in therapeutic antibody or vaccine development, it is critical to assess the quality of immunization-induced polyclonal antibody responses. Here, we developed a workflow that uses sensitive methods to quantitatively and qualitatively assess immune responses against foreign antigens with regard to antibody binding affinity and epitope diversity. The application of such detailed assessments throughout an immunization campaign can significantly reduce the resources required to generate highly specific antibodies. Our workflow consists of the following two steps: 1) the use of surface plasmon resonance to quantify antigen-specific antibodies and evaluate their apparent binding affinities, and 2) the recovery of serum IgGs using an automated small scale purification system, followed by the determination of their epitope diversity using hydrogen deuterium exchange coupled with mass spectrometry. We showed that these methods were sensitive enough to detect antigen-specific IgGs in the nanogram/μl range and that they provided information for differentiating the antibody responses of the various immunized animals that could not be obtained by conventional methods. We also showed that this workflow can guide the selection of an animal that produces high affinity antibodies with a desired epitope coverage profile, resulting in the generation of potential therapeutic monoclonal antibody clones with desirable functional profiles. We postulate that this workflow will be an important tool in the development of effective vaccines to combat the highly sophisticated evasion mechanisms of pathogens.
has issue date	2016-07-29(xsd:dateTime)
bibo:doi	10.1074/jbc.m116.736660
bibo:pmid	27288409
has license	cc-by
sha1sum (hex)	c1cd72dfc8c71714d7751a0b9820f8f8e0ee3756
schema:url	https://doi.org/10.1074/jbc.m116.736660
resource representing a document's title	Efficient Qualitative and Quantitative Determination of Antigen-induced Immune Responses
has PubMed Central identifier	PMC4965583
has PubMed identifier	27288409
schema:publication	J Biol Chem
resource representing a document's body	covid:c1cd72dfc8c71714d7751a0b9820f8f8e0ee3756#body_text
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