About: Multiplex semi-nested RT-PCR with exogenous internal control for simultaneous detection of bovine coronavirus and group A rotavirus

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About: Multiplex semi-nested RT-PCR with exogenous internal control for simultaneous detection of bovine coronavirus and group A rotavirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Multiplex semi-nested RT-PCR with exogenous internal control for simultaneous detection of bovine coronavirus and group A rotavirus
Creator	Asano, Karen Ayres, Giselle Brandão, Paulo Nunes De Barros, Iracema Pinheiro De Souza, Sibele Richtzenhain, Leonardo Oliveira, Sheila Silva, Souza
Source	Elsevier; Medline; PMC
abstract	Neonatal calf diarrhea is a multi-etiology syndrome of cattle and direct detection of the two major agents of the syndrome, group A rotavirus and Bovine coronavirus (BCoV) is hampered by their fastidious growth in cell culture. This study aimed at developing a multiplex semi-nested RT-PCR for simultaneous detection of BCoV (N gene) and group A rotavirus (VP1 gene) with the addition of an internal control (mRNA ND5). The assay was tested in 75 bovine feces samples tested previously for rotavirus using PAGE and for BCoV using nested RT-PCR targeted to RdRp gene. Agreement with reference tests was optimal for BCoV (kappa = 0.833) and substantial for rotavirus detection (kappa = 0.648). the internal control, ND5 mRNA, was detected successfully in all reactions. Results demonstrated that this multiplex semi-nested RT-PCR was effective in the detection of BCoV and rotavirus, with high sensitivity and specificity for simultaneous detection of both viruses at a lower cost, providing an important tool for studies on the etiology of diarrhea in cattle.
has issue date	2010-08-17(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2010.08.008
bibo:pmid	20723564
has license	no-cc
sha1sum (hex)	c21464f238ed092086907bee43f91167c081cfc0
schema:url	https://doi.org/10.1016/j.jviromet.2010.08.008
resource representing a document's title	Multiplex semi-nested RT-PCR with exogenous internal control for simultaneous detection of bovine coronavirus and group A rotavirus
has PubMed Central identifier	PMC7119652
has PubMed identifier	20723564
schema:publication	J Virol Methods
resource representing a document's body	covid:c21464f238ed092086907bee43f91167c081cfc0#body_text
is schema:about of	named entity 'DETECTION' named entity 'RT-PCR' named entity 'samples' named entity 'studies' named entity 'group A' named entity 'RT-PCR' named entity 'tested' named entity 'The' named entity 'effective' named entity 'PAGE' named entity 'etiology' named entity 'MULTIPLEX' named entity 'MRNA' named entity 'ADDITION' named entity 'TESTS' named entity 'DEMONSTRATED' named entity 'ASSAY' named entity 'COST' named entity 'USING' named entity 'THEIR' named entity 'REFERENCE' named entity 'CONTROL' named entity 'GENE' named entity 'STUDIES' named entity '648' named entity 'CATTLE' named entity 'DEVELOPING' named entity 'THE SYNDROME' named entity 'STUDY' named entity 'SIMULTANEOUS' named entity 'GROWTH' named entity 'GROUP A ROTAVIRUS' named entity 'ROTAVIRUS DETECTION' named entity 'BOVINE CORONAVIRUS' named entity 'NESTED' named entity 'CONTROL' named entity 'PREVIOUSLY' named entity 'LOWER' named entity 'EXOGENOUS' named entity 'INTERNAL' named entity 'GROUP A ROTAVIRUS' named entity '833' named entity 'SAMPLES' named entity 'EFFECTIVE' named entity 'DETECTION' named entity 'MAJOR' named entity 'BOVINE CORONAVIRUS' named entity 'DIRECT DETECTION' named entity 'SIMULTANEOUS' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'RT-PCR' named entity 'BOVINE' named entity 'NEONATAL CALF DIARRHEA' named entity 'TARGETED' named entity 'ND5' named entity 'MULTIPLEX' named entity 'AGREEMENT' named entity 'ETIOLOGY' named entity 'IMPORTANT' named entity 'RESULTS' named entity 'RDRP' named entity 'TESTED' named entity 'FECES' named entity 'OPTIMAL' named entity 'DIARRHEA' named entity 'TOOL' named entity 'PROVIDING' named entity 'INTERNAL' named entity 'VIRUSES' named entity 'MULTI' named entity 'ROTAVIRUS' named entity 'NESTED' named entity 'PAGE' named entity 'IS A'

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