About: Recombinant cold shock domain containing protein is a potential antigen to detect specific antibody during early and late infections of Haemonchus contortus in goat

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About: Recombinant cold shock domain containing protein is a potential antigen to detect specific antibody during early and late infections of Haemonchus contortus in goat Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Recombinant cold shock domain containing protein is a potential antigen to detect specific antibody during early and late infections of Haemonchus contortus in goat
Creator	Yan, Song, Xiangrui Hasan, Sana Aimulajiang, Muhammad Chu, Lixin Lakho, Wen Li, Ruofeng Memon, Muhammad, Ali-Ul-Husnain Muhammad, Waqqas Naqvi, Kalibixiati Naqvi, Shakeel Xu, Xiaokai
Source	PMC
abstract	BACKGROUND: Haemonchus contortus (H. contortus) is one of the most important parasites that cause huge economic losses to small ruminant industry worldwide. Effective prognosis and treatment depend upon the early diagnosis of H. contortus infection. To date, no widely-approved methods for the identification of prepatent H. contortus infection are available to identify prepatent H. contortus infection properly. The aim of this study was to evaluate the diagnostic potential of recombinant cold shock H. contortus protein (rHc-CS) during early and late infections of H. contortus in goat. RESULTS: Purified rHc-CS exhibited a clear band, with a molecular weight about 38 kDa. H. contortus eggs were not detected by fecal egg count technique from feces collected at 0 to 14 days post infection (D.P.I). However, eggs were detected at 21, 28 and 35 D.P.I. Hence, results of immunoblotting assay showed specific anti rHc-CS antibody detection in all goat sera collected at early stage (14 D.P.I) and late stage (21–103 D.P.I) of H. contortus infection. Furthermore, no cross reactivity was observed against Trichinella spiralis, Fasciola hepatica and Toxoplasma gondii or uninfected goats. Among several evaluated rHc-CS indirect-ELISA format variables, favorable antigen coating concentration was found 0.28 μg/well at 37 °C 1 h and overnight at 4 °C. Moreover, optimum dilution ratio of serum and rabbit anti-goat IgG was recorded as 1:100 and 1:4000, respectively. The best blocking buffer was 5% Bovine Serum Albumin (BSA) while the best time for blocking, serum incubation and TMB reaction were recorded as 60, 120 and 10 min, respectively. The cut-off value for positive and negative interpretation was determined as 0.352 (OD(450)). The diagnostic specificity and sensitivity of the rHc-CS, both were recorded as 100%. CONCLUSION: These results validated that rHc-CS is a potential immunodiagnostic antigen to detect the specific antibodies during early and late H. contortus infections in goat.
has issue date	2020-02-03(xsd:dateTime)
bibo:doi	10.1186/s12917-020-2261-6
bibo:pmid	32013987
has license	cc-by
sha1sum (hex)	c22900bb9a1c84d2bb1a1dd49af316a3f3030a79
schema:url	https://doi.org/10.1186/s12917-020-2261-6
resource representing a document's title	Recombinant cold shock domain containing protein is a potential antigen to detect specific antibody during early and late infections of Haemonchus contortus in goat
has PubMed Central identifier	PMC6998305
has PubMed identifier	32013987
schema:publication	BMC Vet Res
resource representing a document's body	covid:c22900bb9a1c84d2bb1a1dd49af316a3f3030a79#body_text
is schema:about of	named entity 'feces' named entity 'serum' named entity 'early' named entity 'variables' named entity 'eggs' named entity 'sensitivity' named entity 'Conclusion' named entity 'anti' named entity 'collected' named entity 'infections' named entity 'industry' named entity 'early' named entity 'Haemonchus contortus' named entity 'EARLY' named entity 'INCUBATION' named entity 'D.P.' named entity 'DETECTED' named entity 'TOXOPLASMA GONDII' named entity 'BEST' named entity '28H' named entity 'BUFFER' named entity 'ASSAY' named entity '352' named entity 'IMPORTANT' named entity '100%' named entity 'CUT-OFF VALUE' named entity 'DILUTION RATIO' named entity 'EGG COUNT' named entity 'POTENTIAL' named entity 'SENSITIVITY' named entity 'ANTIGEN' named entity 'DEPEND' named entity 'CONCENTRATION' named entity '0.28' named entity 'TRICHINELLA SPIRALIS' named entity 'ELISA' named entity 'APPROVED' named entity 'METHODS' named entity 'DIAGNOSTIC' named entity 'DIAGNOSTIC SPECIFICITY' named entity 'RESULTS' named entity 'CAUSE' named entity 'HAEMONCHUS CONTORTUS' named entity 'INDIRECT' named entity '450' covid:arg/c22900bb9a1c84d2bb1a1dd49af316a3f3030a79 named entity 'goat' named entity 'kDa' named entity 'time' named entity 'recombinant' named entity 'IgG' named entity 'days' named entity 'parasites' named entity 'protein' named entity 'late' named entity 'antigen' named entity 'band' named entity 'validated' named entity 'antibodies' named entity 'sera' named entity 'infection' named entity 'overnight' named entity 'These' named entity 'blocking' named entity 'infection' named entity 'concentration' named entity 'worldwide' named entity 'positive' named entity 'depend'

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