About: Detection of SARS-CoV-2 in formalin-fixed paraffin-embedded tissue sections using commercially available reagents

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About: Detection of SARS-CoV-2 in formalin-fixed paraffin-embedded tissue sections using commercially available reagents Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection of SARS-CoV-2 in formalin-fixed paraffin-embedded tissue sections using commercially available reagents
Creator	Christopher, • Larsen, P Mukhopadhyay, • Rocha, Alejandro Barton, Lisa Caza, Tiffany Duval, Eric Kenan, Daniel Kuperman, • Sharma, Shree Stroberg, • Wilson, Jon Yarid, Nicole
Source	Medline; PMC
abstract	Coronavirus Disease-19 (COVID-19), caused by the coronavirus SARS-CoV-2, was initially recognized in Wuhan, China and subsequently spread to all continents. The disease primarily affects the lower respiratory system, but may involve other organs and systems. Histopathologic evaluation of tissue from affected patients is crucial for diagnostic purposes, but also for advancing our understanding of the disease. For that reason, we developed immunohistochemical (IHC) and in situ hybridization (ISH) assays for detection of the. virus. A total of eight autopsy lungs, one placenta, and ten kidney biopsies from COVID-19 patients were stained with a panel of commercially available antibodies for IHC and commercially available RNA probes for ISH. Similarly, autopsy lungs, placentas and renal biopsies from non-COVID-19 patients were stained with the same antibodies and probes. All eight lungs and the placenta from COVID-19 patients stained positive by IHC and ISH, while the kidney biopsies stained negative by both methodologies. As expected, all specimens from non-COVID-19 patients were IHC and ISH negative. These two assays represent a sensitive and specific method for detecting the virus in tissue samples. We provide the protocols and the list of commercially available antibodies and probes for these assays, so they can be readily implemented in pathology laboratories and medical examiner offices for diagnostic and research purposes.
has issue date	2020-07-09(xsd:dateTime)
bibo:doi	10.1038/s41374-020-0464-x
bibo:pmid	32647285
has license	no-cc
sha1sum (hex)	c236997ed332f373331a244002290a43760a7b54
schema:url	https://doi.org/10.1038/s41374-020-0464-x
resource representing a document's title	Detection of SARS-CoV-2 in formalin-fixed paraffin-embedded tissue sections using commercially available reagents
has PubMed Central identifier	PMC7344352
has PubMed identifier	32647285
schema:publication	Lab Invest
resource representing a document's body	covid:c236997ed332f373331a244002290a43760a7b54#body_text
is schema:about of	named entity 'Histopathologic' named entity 'antibodies' named entity 'ten' named entity 'ANTIBODIES' named entity 'HISTOPATHOLOGIC' named entity 'commercially' named entity 'initially' named entity 'Coronavirus' named entity 'kidney' named entity 'biopsies' named entity 'laboratories' named entity 'expected' named entity 'immunohistochemical' named entity 'total' named entity 'virus' named entity 'samples' named entity 'patients' named entity 'All' named entity 'IHC' named entity 'detection' named entity 'tissue' named entity 'tissue samples' named entity 'virus' named entity 'antibodies' named entity 'China' named entity 'Coronavirus' named entity 'COVID' named entity 'pathology' named entity 'DAB' named entity 'SARS-CoV-2' named entity 'ISH' named entity 'medical examiner' named entity 'Leica' named entity 'SARS-CoV-2' named entity 'COVID' named entity 'RT-PCR' named entity 'ISH' named entity 'sensitive and specific' named entity 'autopsy' named entity 'genitourinary' named entity 'placenta' named entity 'SARS-CoV-2' named entity 'organ' named entity 'lung' named entity 'kidney' named entity 'glycoproteins' named entity 'signs and symptoms' named entity 'pneumocytes' named entity 'post-mortem' named entity 'Newark' named entity 'gastrointestinal' named entity 'hematoxylin' named entity 'incubation' named entity 'PPIB' named entity 'staining' named entity 'Coronavirus Disease 2019' named entity 'SARS-CoV-2' named entity 'non-specific' named entity 'antibodies' named entity 'COVID' named entity 'SARS-CoV-2' named entity 'endothelial cells' named entity 'Declaration of Helsinki' named entity 'formalin-fixed' named entity 'study protocol' named entity 'COVID' named entity 'nervous systems' named entity 'Wetzlar' named entity 'cardiovascular'

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