About: Evaluation of ViroCyt(®) Virus Counter for Rapid Filovirus Quantitation

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About: Evaluation of ViroCyt(®) Virus Counter for Rapid Filovirus Quantitation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluation of ViroCyt(®) Virus Counter for Rapid Filovirus Quantitation
Creator	Norwood, David Heinrich, Megan Ingram, Michael Kearney, Brian Kuhn, Jens Olschner, Scott Robinson, Camenzind Rossi, Cynthia Schoepp, Randal Williams, Priscilla Zovanyi, Ashley
Source	Medline; PMC
abstract	Development and evaluation of medical countermeasures for diagnostics, vaccines, and therapeutics requires production of standardized, reproducible, and well characterized virus preparations. For filoviruses this includes plaque assay for quantitation of infectious virus, transmission electron microscopy (TEM) for morphology and quantitation of virus particles, and real-time reverse transcription PCR for quantitation of viral RNA (qRT-PCR). The ViroCyt(®) Virus Counter (VC) 2100 (ViroCyt, Boulder, CO, USA) is a flow-based instrument capable of quantifying virus particles in solution. Using a proprietary combination of fluorescent dyes that stain both nucleic acid and protein in a single 30 min step, rapid, reproducible, and cost-effective quantification of filovirus particles was demonstrated. Using a seed stock of Ebola virus variant Kikwit, the linear range of the instrument was determined to be 2.8E+06 to 1.0E+09 virus particles per mL with coefficient of variation ranging from 9.4% to 31.5% for samples tested in triplicate. VC particle counts for various filovirus stocks were within one log of TEM particle counts. A linear relationship was established between the plaque assay, qRT-PCR, and the VC. VC results significantly correlated with both plaque assay and qRT-PCR. These results demonstrated that the VC is an easy, fast, and consistent method to quantify filoviruses in stock preparations.
has issue date	2015-02-20(xsd:dateTime)
bibo:doi	10.3390/v7030857
bibo:pmid	25710889
has license	cc-by
sha1sum (hex)	c2b8b47dcfe70db8080da73d7a3941c94b31b025
schema:url	https://doi.org/10.3390/v7030857
resource representing a document's title	Evaluation of ViroCyt(®) Virus Counter for Rapid Filovirus Quantitation
has PubMed Central identifier	PMC4379551
has PubMed identifier	25710889
schema:publication	Viruses
resource representing a document's body	covid:c2b8b47dcfe70db8080da73d7a3941c94b31b025#body_text
is schema:about of	named entity 'RAPID' named entity '30 MIN' named entity 'PROPRIETARY' named entity 'RAPID' named entity '9.4' named entity 'USING' named entity 'PCR' named entity 'STEP' named entity 'VIRUS PARTICLES' named entity 'COMBINATION' named entity 'VACCINES' named entity 'VARIOUS' named entity 'VIRUS' named entity 'STAIN' named entity 'INSTRUMENT' named entity 'FILOVIRUSES' named entity 'TESTED' named entity 'COUNTS' named entity 'FLOW' named entity 'NUCLEIC ACID' named entity 'TRIPLICATE' named entity 'RELATIONSHIP' named entity 'TEM' named entity 'DEMONSTRATED' named entity 'PRODUCTION' named entity 'TRANSMISSION ELECTRON MICROSCOPY' named entity 'PLAQUE ASSAY' named entity 'EVALUATION' named entity 'SINGLE' named entity 'BOULDER' named entity 'VARIANT' named entity 'LINEAR' named entity 'REVERSE TRANSCRIPTION PCR' named entity 'PARTICLES' named entity 'MEDICAL COUNTERMEASURES' named entity 'CAPABLE' named entity 'EVALUATION' named entity 'INFECTIOUS' named entity 'PARTICLE' named entity 'EFFECTIVE' named entity 'STOCK' named entity 'THERAPEUTICS' named entity 'CHARACTERIZED' named entity 'COUNTER' named entity 'VIRUS' named entity 'PER ML' named entity 'VIRAL RNA' named entity 'COEFFICIENT OF VARIATION' named entity 'INCLUDES' named entity 'LOG' named entity 'BASED' named entity 'SEED' named entity 'DETERMINED' named entity 'COUNTER' named entity 'SAMPLES' named entity 'QUANTITATION' named entity 'REAL-TIME' named entity 'QUANTITATION' named entity 'PROTEIN ' named entity 'MORPHOLOGY' named entity 'SOLUTION' named entity 'USA' named entity 'FILOVIRUS' named entity 'DIAGNOSTICS' named entity 'EBOLA VIRUS' named entity 'LINEAR RANGE' named entity 'DEVELOPMENT' named entity 'FILOVIRUS' named entity 'FLUORESCENT DYES' named entity 'IS A' named entity 'COST'

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